【摘要】目的觀察供肝轉(zhuǎn)染IL-10后基因表達(dá)情況。
方法應(yīng)用改良“二袖套法”行Lewis到BN大鼠肝移植11例，然后分為對照組3例； 空載體轉(zhuǎn)染組4例，術(shù)中供肝冷保存期門靜脈注射Lipofectamine 2000-pCR3.1空載體質(zhì)粒復(fù)合物，保存45 min后行肝移植； 重組IL-10(rIL-10)轉(zhuǎn)染組4例，術(shù)中供肝冷保存期門靜脈注射Lipofectamine 2000-pCR3.1 rIL-10復(fù)合物，保存45 min后行肝移植。術(shù)后第6天處死全部大鼠，取血清檢測IL-10水平，另取肝組織行免疫組化染色和RT-PCR檢測肝細(xì)胞IL-10表達(dá)水平。
結(jié)果rIL-10轉(zhuǎn)染組血清IL-10水平明顯升高，肝上下腔靜脈IL-10水平可達(dá)（639.27±67.11） pg/ml，是肝下下腔靜脈IL-10水平的近1.4倍（P=0.024）； 免疫組化染色結(jié)果示肝細(xì)胞胞槳呈棕黃色或深棕色，而其他兩組肝細(xì)胞著色輕微或不明顯。肝組織RT-PCR顯示，IL-10 mRNA的表達(dá)在rIL-10轉(zhuǎn)染組明顯處于高水平（P=0.000）。
結(jié)論脂質(zhì)體介導(dǎo)，體外冷保存期經(jīng)門靜脈途徑進(jìn)行供肝轉(zhuǎn)染IL-10，可以使IL-10在肝臟獲得較高水平的表達(dá)。
引用本文： 張棟,高東宸,張忠濤,王宇. 大鼠肝移植中脂質(zhì)體介導(dǎo)IL-10基因轉(zhuǎn)染供肝的實(shí)驗(yàn)研究. 中國普外基礎(chǔ)與臨床雜志, 2005, 12(2): 123-126. doi: 復(fù)制
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