• 1.華中科技大學(xué)附屬同濟(jì)醫(yī)院器官移植研究所（武漢430030）;;
  • 2.中南大學(xué)附屬湘雅三醫(yī)院移植醫(yī)學(xué)研究院（長沙410013）;

【摘要】目的探討三七總皂苷（PNS）預(yù)處理對大鼠供肝缺血-再灌注損傷的保護(hù)作用及其對供肝細(xì)胞凋亡和Bcl-2及Caspase-3 mRNA表達(dá)的影響。
方法雄性SD大鼠分別用作供、受體，采用Kamada’s袖套法建立原位肝移植模型，根據(jù)供肝切取前1 h是否靜脈注射PNS（50 mg/kg）將大鼠隨機(jī)分為PNS預(yù)處理組（PNS組）和生理鹽水對照組（NS組）； 另設(shè)假手術(shù)作對照組（SO組）。分別于供肝再灌注后 2 h、6 h及24 h處死各組動物，檢測血清ALT及AST，HE切片作病理組織學(xué)檢查，TUNEL法檢測肝細(xì)胞凋亡，RT-PCR法檢測Bcl-2及Caspase-3 mRNA的表達(dá)。
結(jié)果供肝再灌注后2 h、6 h及24 h各時點，PNS組大鼠血清ALT和AST水平及肝細(xì)胞凋亡指數(shù)（AI）均明顯低于NS組（P＜0.05，P＜0.01）； 6 h及24 h，PNS組大鼠肝組織Bcl-2 mRNA的表達(dá)水平明顯高于NS組（P＜0.05）； 2 h及6 h，PNS組大鼠肝組織Caspase-3 mRNA的表達(dá)水平明顯低于NS組（P＜0.05）。
結(jié)論P(yáng)NS預(yù)處理大鼠供肝，可以有效地減輕移植肝的缺血/再灌注損傷和細(xì)胞凋亡，影響細(xì)胞凋亡調(diào)控基因Bcl-2和 Caspase-3的表達(dá)。這可能為PNS抗細(xì)胞凋亡的機(jī)理之一。

引用本文： 張毅,葉啟發(fā),周進(jìn)學(xué),明英姿,許賢林,肖建生. 三七總皂苷對大鼠肝移植物細(xì)胞凋亡及Bcl-2和Caspase-3 表達(dá)的影響. 中國普外基礎(chǔ)與臨床雜志, 2005, 12(2): 113-116下轉(zhuǎn)126. doi: 復(fù)制

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  2. 2. Kamada N, Calne RY. Orthotopic liver transplantation in the rat. Technique using cuff for portal vein anastomosis and biliary drainage [Ｊ]. Transplantation, 1979; 28(1)47.
  3. 3. Kohli V, Selzner M, Madden JF, et al. Endothelial cell and hepatocyte deaths occur by apoptosis after ischemiareperfusion injury in the rat liver [Ｊ]. Transplantation, 1999; 67(8)1099.
  4. 4. 朱陵群，范吉平，黃啟福， 等. 三七總皂苷抗缺氧缺糖再給氧誘導(dǎo)大鼠海馬神經(jīng)細(xì)胞凋亡的研究 [Ｊ]. 中國中藥雜志, 2003； 28（1）52.
  5. 5. Gross A, McDonnell JM, Korsmeyer SJ. BCL2 family members and the mitochondria in apoptosis [Ｊ]. Genes Dev, 1999; 13(15)1899.
  6. 6. Bilbao G, Contreras JL, Eckhoff DE, et al. Reduction of ischemiareperfusion injury of the liver by in vivo adenovirusmediated gene transfer of the antiapoptotic Bcl2 gene [Ｊ]. Ann Surg, 1999; 230(2)185.
  7. 7. SS〗（下轉(zhuǎn)第126頁） （上承第116頁）.
  8. 8. Oshiro T, Shiraishi M, Muto Y. Adenovirus mediated gene transfer of antiapoptotic protein in hepatic ischemiareperfusion injury: the paradoxical effect of Bcl2 expression in the reperfused liver [Ｊ]. J Surg Res, 2002; 103(1)30.
  9. 9. Fumarola C, Guidotti GG. Stressinduced apoptosis: toward a symmetry with receptormediated cell death [Ｊ]. Apoptosis, 2004; 9(1)77.
  10. 10. Cursio R, Gugenheim J, Ricci JE, et al. Caspase inhibition protects from liver injury following ischemia and reperfusion in rats [Ｊ]. Transpl Int, 2000; 13(Suppl 1)S568.
  11. 11. 李巾偉，朱培純，司銀楚,等. 三七總皂甙對腦出血大鼠前腦促凋亡基因Caspase3影響的研究 [Ｊ]. 北京中醫(yī)藥大學(xué)學(xué)報, 2003； 26（2）22.