• 哈爾濱醫(yī)科大學附屬腫瘤醫(yī)院普外科(哈爾濱150040);

目的　觀察一氧化氮合酶（NOS）抑制劑N-硝基精氨酸甲酯(L-NAME)抑制大腸癌生長的體內(nèi)效應，及其對大腸癌移植瘤相關血管形成的影響。方法　將20只BALB/c（nu/nu）裸鼠按隨機數(shù)字表法均分為對照組和實驗組,大腸癌細胞系SL174T種植入裸鼠皮下，形成移植瘤。對照組0.2 ml生理鹽水灌胃,實驗組經(jīng)口灌入L-NAME,4 mg/次，3次/周，共4周。然后記錄腫瘤大小，采用免疫組化法檢測微血管密度。結果　L-NAME對裸鼠大腸癌移植瘤的腫瘤重量抑瘤率為41.36%； 腫瘤體積抑瘤率為43.48%。實驗組大腸癌組織微血管密度（14.83±2.10）個，明顯低于對照組的（21.04±3.11）個，兩組間差異有統(tǒng)計學意義（P＜0.05）。結論　L-NAME具有抑制裸鼠大腸癌腫瘤生長和對抗腫瘤新生血管生成的作用。

引用本文： 于麗波,孫文洲,董新舒. N-硝基精氨酸甲酯對大腸癌裸鼠移植瘤生長的抑制作用△. 中國普外基礎與臨床雜志, 2006, 13(5): 542-544. doi: 復制

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  3. 3. 　Kojima M, Morisaki T, Tsukahara Y, et al. Nitric oxide synthase expression and nitric oxide production in human colon carcinoma tissue [Ｊ]. J Surg Oncol, 1999; 70(4)∶ 222.
  4. 4. 　李學農(nóng)， 周　軍， 丁彥青. 一氧化氮合酶在人大腸癌細胞中的表迭及意義 [Ｊ]. 中國腫瘤臨床， 2001； 28（2）∶ 94.
  5. 5. 　Lagares-Garcia JA, Moore RA, Collier B, et al. Nitric oxide synthase as a marker in colorectal carcinoma [Ｊ].Am Surg, 2001; 67(7)∶ 709.
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  8. 8. 　Orucevic A, Lala PK. Effects of N(G)-Nitro-L-arginine methyl ester, an inhibitor of nitric oxide synthesis, on IL-2-induced LAK cell generation in vivo and in vitro in healthy and tumor-bearing mice [Ｊ]. Cell Immunol, 1996; 169(1)∶ 125.
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