• 1.中國(guó)醫(yī)科大學(xué)附屬第一醫(yī)院普外科（沈陽110001）;;
  • 2.沈陽醫(yī)學(xué)院奉天醫(yī)院普外科（沈陽110024）;;
  • 3.中國(guó)醫(yī)科大學(xué)附屬第二醫(yī)院普外科（沈陽110004）;;
  • 通訊作者： 李航宇;

目的探討肝細(xì)胞膽管膜上轉(zhuǎn)運(yùn)子BSEP、MRP2和MDR3與膽囊膽固醇結(jié)石形成的關(guān)系。
方法收集膽囊膽固醇結(jié)石患者的肝組織標(biāo)本20例，正常肝組織標(biāo)本10例。應(yīng)用逆轉(zhuǎn)錄聚合酶鏈反應(yīng)（RTPCR）技術(shù)和Western blot技術(shù)檢測(cè)BSEP、MRP2及MDR3 mRNA和蛋白的表達(dá)。
結(jié)果膽囊膽固醇結(jié)石患者肝組織中BSEP、MRP2和MDR3 的mRNA及蛋白表達(dá)均顯著低于正常肝組織（P＜0.01）。
結(jié)論 BSEP、MRP2和MDR3的表達(dá)降低可能與膽囊膽固醇結(jié)石的形成有關(guān)。

引用本文： 孔凡民,隋春陽,李航宇,李昱驥,孫宏治,郭仁宣,郭克建,田雨霖. 膽囊膽固醇結(jié)石與肝細(xì)胞膽管膜上轉(zhuǎn)運(yùn)子BSEP、MRP2和MDR3關(guān)系的研究. 中國(guó)普外基礎(chǔ)與臨床雜志, 2006, 13(3): 314-316. doi: 復(fù)制

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7. Plass JR, Mol O, Heegsma J, et al. A progressive familial intrahepatic cholestasis type 2 mutation causes an unstable, temperaturesensitive bile salt export pump [Ｊ]. J Hepatol， 2004; 40(1)∶24.
8. Fickert P, Fuchsbichler A, Wagner M, et al. Regurgitation of bile acids from leaky bile ducts causes sclerosing cholangitis in Mdr2 (Abcb4) knockout mice [Ｊ]. Gastroenterology, 2004; 127(1)∶261.
9. Shoda J, Oda K, Suzuki H, et al. Etiologic significance of defects in cholesterol, phospholipid, and bile acid metabolism in the liver of patients with intrahepatic calculi [Ｊ]. Hepatology, 2001; 33(5)∶1194.
10. Smit JJ, Schinkel AH, Oude Elferink RP, et al. Homozygous disruption of the murine mdr2 Pglycoprotein gene leads to a complete absence of phospholipid from bile and to liver disease [Ｊ]. Cell, 1993; 75(3)∶451.
11. 劉君， 龍厚勇， 明晗昕， 等. 肝細(xì)胞MDR1基因表達(dá)與膽囊膽固醇結(jié)石相關(guān)性研究 [Ｊ]. 中國(guó)普外基礎(chǔ)與臨床雜志， 2005； 12（4）∶346.
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  2. 2. van BergeHenegouwen GP, Venneman NG, Portincasa P, et al. Relevance of hereditary defects in lipid transport proteins for the pathogenesis of cholesterol gallstone disease.
  3. 3. Trauner M, Boyer JL. Bile salt transporters: molecular characterization, function, and regulation [Ｊ]. Physiol Rev, 2003; 83(2)∶633.
  4. 4. Hoda F, Green RM. Hepatic canalicular membrane transport of bile salt in C57L/J and AKR/J mice: implications for cholesterol gallstone formation [Ｊ]. J Membr Biol， 2003; 196(1)∶9.
  5. 5. Huang L, Zhao A, Lew JL, et al. Farnesoid X receptor activates transcription of the phospholipid pump MDR3 [Ｊ]. J Biol Chem, 2003; 278(51)∶51085.
  6. 6. Meier PJ, Stieger B. Bile salt transporters [Ｊ]. Annu Rev Physiol， 2002; 64(2)∶635.
  7. 7. Plass JR, Mol O, Heegsma J, et al. A progressive familial intrahepatic cholestasis type 2 mutation causes an unstable, temperaturesensitive bile salt export pump [Ｊ]. J Hepatol， 2004; 40(1)∶24.
  8. 8. Fickert P, Fuchsbichler A, Wagner M, et al. Regurgitation of bile acids from leaky bile ducts causes sclerosing cholangitis in Mdr2 (Abcb4) knockout mice [Ｊ]. Gastroenterology, 2004; 127(1)∶261.
  9. 9. Shoda J, Oda K, Suzuki H, et al. Etiologic significance of defects in cholesterol, phospholipid, and bile acid metabolism in the liver of patients with intrahepatic calculi [Ｊ]. Hepatology, 2001; 33(5)∶1194.
  10. 10. Smit JJ, Schinkel AH, Oude Elferink RP, et al. Homozygous disruption of the murine mdr2 Pglycoprotein gene leads to a complete absence of phospholipid from bile and to liver disease [Ｊ]. Cell, 1993; 75(3)∶451.
  11. 11. 劉君， 龍厚勇， 明晗昕， 等. 肝細(xì)胞MDR1基因表達(dá)與膽囊膽固醇結(jié)石相關(guān)性研究 [Ｊ]. 中國(guó)普外基礎(chǔ)與臨床雜志， 2005； 12（4）∶346.