• 1.中國(guó)醫(yī)科大學(xué)盛京醫(yī)院肝膽外科（沈陽(yáng)110004）;;
  • 2.中國(guó)醫(yī)科大學(xué)第一臨床學(xué)院附院核醫(yī)學(xué)科（沈陽(yáng)110001）;;
  • 3.中國(guó)原子能研究院同位素研究所（北京100243）;;
  • 4.中國(guó)醫(yī)科大學(xué)盛京醫(yī)院中心實(shí)驗(yàn)室;;
  • 5.中國(guó)醫(yī)科大學(xué)盛京醫(yī)院病理科;

目的 　探討103鈀（103Pd）放射性支架釋放的γ射線對(duì)Fas表達(dá)的影響以及與膽管癌細(xì)胞凋亡的關(guān)系及意義。
方法 　培養(yǎng)瓶中膽管癌細(xì)胞消化后形成細(xì)胞懸液，采用血細(xì)胞計(jì)數(shù)法計(jì)數(shù)細(xì)胞，按1×105/ml的濃度分裝到2 ml塑料凍存管中。分別設(shè)置普通支架組及103Pd支架組，應(yīng)用TUNEL法和免疫組化染色方法檢測(cè)γ射線誘導(dǎo)膽管癌細(xì)胞凋亡的情況和Fas表達(dá)的情況。
結(jié)果 　103Pd支架組膽管癌細(xì)胞的Fas表達(dá)較普通支架組明顯增高（P＜0.05），且膽管癌細(xì)胞出現(xiàn)明顯細(xì)胞凋亡，凋亡細(xì)胞數(shù)明顯高于普通支架組（P＜0.01）。
結(jié)論 　Fas表達(dá)水平與膽管癌細(xì)胞凋亡的發(fā)生有關(guān)，103Pd放射性支架可能通過(guò)增強(qiáng)Fas表達(dá)，促進(jìn)膽管癌細(xì)胞凋亡，從而為臨床應(yīng)用103Pd放射性支架治療膽管癌提供理論依據(jù)。

引用本文： 何貴金,于發(fā)強(qiáng),高沁怡,許書(shū)河,高紅,姜維國(guó),蔣濤,戴顯偉,戴朝六. 103鈀放射性支架對(duì)Fas基因表達(dá)影響及與膽管癌細(xì)胞凋亡關(guān)系研究. 中國(guó)普外基礎(chǔ)與臨床雜志, 2007, 14(3): 284-286. doi: 復(fù)制

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2. Peschel RE, Colberg JW, Chen Z, et al. Iodine 125 versus palladium 103 implants for prostate cancer: clinical outcomes and complications [J]. Cancer J， 2004; 10(3)∶170.
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6. Bittner RM, Crowe SF. The relationship between naming difficulty and FAS performance following traumatic brain injury [J]. Brain Inj， 2006; 20(9)∶971.
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9. McVicker BL, Tuma DJ, Kubik JL, et al. Ethanol-induced apoptosis in polarized hepatic cells possibly through regulation of the Fas pathway [J]. Alcohol Clin Exp Res， 2006; 30(11)∶1906.
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11. Oshimi Y, Miyazaki S. Fas antigen-mediated DNA fragmentation and apoptotic morphologic changes are regulated by elevated cytosolic Ca2+ level [J]. J Immunol， 1995; 154(2)∶599.
12. Venet F, Pachot A, Debard AL, et al. Human CD4+CD25+ regulatory T lymphocytes inhibit lipopolysaccharide-induced monocyte survival through a Fas/Fas ligand-dependent mechanism [J]. J Immunol， 2006; 177(9)∶6540.
13. Chmura SJ, Nodzenski E, Beckett MA, et al. Loss of ceramide production confers resistance to radiation-induced apoptosis [J]. Cancer Res， 1997; 57(7)∶1270.
14. Gadbois DM, Crissman HA, Nastasi A, et al. Alterations in the progression of cells through the cell cycle after exposure to alpha particles or gamma rays [J]. Radiat Res， 1996; 146(4)∶414.
15. Petri S, Kiaei M, Wille E, et al. Loss of Fas ligand-function improves survival in G93A-transgenic ALS mice [J]. J Neurol Sci， 2006; 251(1-2)∶44.
  1. 1. Itamochi H, Yamasaki F, Sudo T, et al. Reduction of radiation-induced apoptosis by specific expression of Bcl-2 in normal cells [J]. Cancer Gene Ther， 2006; 13(5)∶451.
  2. 2. Peschel RE, Colberg JW, Chen Z, et al. Iodine 125 versus palladium 103 implants for prostate cancer: clinical outcomes and complications [J]. Cancer J， 2004; 10(3)∶170.
  3. 3. Grigoriu M, Palade R, Vasile D, et al. Technic, indications and contribution of choledochoscopy in the diagnosis and treatment of biliary diseases [J]. Chirurgia (Bucur)， 2003; 98(2)∶167.
  4. 4. Wiedermann JG, Marboe C, Amols H, et al. Intracoronary irradiation markedly reduces restenosis after balloon angioplasty in a porcine model [J]. J Am Coll Cardiol， 1994; 23(6)∶1491.
  5. 5. 李海民， 竇科峰， 李韌, 等. 肝癌組織中Fas和FasL mRNA表達(dá)水平的變化及其意義 [J]. 中國(guó)普外基礎(chǔ)與臨床雜志, 2005; 12（3）∶284.
  6. 6. Bittner RM, Crowe SF. The relationship between naming difficulty and FAS performance following traumatic brain injury [J]. Brain Inj， 2006; 20(9)∶971.
  7. 7. Drewa T, Wolski Z, Skok Z, et al. The FAS-related apoptosis signaling pathway in the prostate intraepithelial neoplasia and cancer lesions [J]. Acta Pol Pharm， 2006; 63(4)∶311.
  8. 8. Protonotariou E, Rizos D, Malamitsi-Puchner A, et al. Tissue polypeptide specific antigen and soluble Fas during normal pregnancy and early life [J]. In Vivo， 2006; 20(6B)∶901.
  9. 9. McVicker BL, Tuma DJ, Kubik JL, et al. Ethanol-induced apoptosis in polarized hepatic cells possibly through regulation of the Fas pathway [J]. Alcohol Clin Exp Res， 2006; 30(11)∶1906.
  10. 10. Weissman JL. Old problems, new techniques: the interventional radiologist and the lacrimal apparatus [J]. Radiology， 1999; 212(2)∶305.
  11. 11. Oshimi Y, Miyazaki S. Fas antigen-mediated DNA fragmentation and apoptotic morphologic changes are regulated by elevated cytosolic Ca2+ level [J]. J Immunol， 1995; 154(2)∶599.
  12. 12. Venet F, Pachot A, Debard AL, et al. Human CD4+CD25+ regulatory T lymphocytes inhibit lipopolysaccharide-induced monocyte survival through a Fas/Fas ligand-dependent mechanism [J]. J Immunol， 2006; 177(9)∶6540.
  13. 13. Chmura SJ, Nodzenski E, Beckett MA, et al. Loss of ceramide production confers resistance to radiation-induced apoptosis [J]. Cancer Res， 1997; 57(7)∶1270.
  14. 14. Gadbois DM, Crissman HA, Nastasi A, et al. Alterations in the progression of cells through the cell cycle after exposure to alpha particles or gamma rays [J]. Radiat Res， 1996; 146(4)∶414.
  15. 15. Petri S, Kiaei M, Wille E, et al. Loss of Fas ligand-function improves survival in G93A-transgenic ALS mice [J]. J Neurol Sci， 2006; 251(1-2)∶44.