• 大連醫(yī)科大學第一附屬醫(yī)院普通外科(大連 116011);

目的  探討谷氨酰胺對醋酸損傷的大鼠畸形腺窩灶的影響及其機理。
方法  Wistar大鼠30只均分成對照組、醋酸組和谷氨酰胺組3組。 用1%醋酸灌注大鼠結腸制成大鼠結腸損傷模型,谷氨酰胺組在制模后第2 d 開始給予谷氨酰胺灌胃。 各組于制模14 d后處死動物,取損傷結腸組織進行HE染色,觀察病理組織學變化; 對所取的結腸隱窩用鹽酸浸潤消化法分離,于掃描電鏡下觀察隱窩情況; 用免疫組織化學方法檢測結腸黏膜上皮細胞中CD44和細胞間黏附分子-1(intercellular adhesion molecule-1, ICAM-1)的表達情況。
結果  制模后14 d, 醋酸組畸形腺窩灶數(shù)目較谷氨酰胺組和對照組多,且呈分叉狀。 谷氨酰胺組結腸黏膜上皮細胞中ICAM-1和CD44表達陽性細胞數(shù)(302.1±30.1、 298.6±28.3)均明顯高于對照組(223.6±23.5、 221.5±28.6)和醋酸組(198.5±19.5、 215.3±17.8),P<0.05; 而后2組間差異無統(tǒng)計學意義(P>0.05)。
結論  谷氨酰胺能減少醋酸損傷大鼠畸形腺窩灶的形成; 增加CD44和ICAM-1的表達,可能是谷氨酰胺能夠減少其形成的一個重要因素。

引用本文: 程雷,譚廣,董擂,李克軍,王忠裕. 谷氨酰胺對實驗性大鼠畸形腺窩灶的影響及其機理研究. 中國普外基礎與臨床雜志, 2008, 15(8): 600-602. doi: 復制

版權信息: ?四川大學華西醫(yī)院華西期刊社《中國普外基礎與臨床雜志》版權所有,未經授權不得轉載、改編

1.  劉斌, 郝志英. 谷氨酰胺在腫瘤治療中的應用進展[J]. 腫瘤研究與臨床, 2006; 18(3)∶ 207.
2.  程蕾, 來茂德. 一種新的結直腸癌癌前病變—畸形腺窩灶[J]. 臨床與實驗病理學雜志, 2001; 17(3)∶ 253.
3.  Araki K, Kobayashi M, Ogata T. A scanning electron microscopic study of the three-dimensional configuration of isolated crypts from human colon adenomas [J]. Med Electron Microsc, 1994; 27(1)∶ 55.
4.  Inoué T, Osatake H. A new drying method of biological specimens for scanning electron microscopy: the t-butyl alcohol freeze-drying method [J]. Arch Histol Cytol, 1988; 51(1)∶ 53.
5.  Moon C, Jeong CW, Kim H, et al. Expression of CD44 adhesion molecule in rat testis with ischemia/reperfusion injury [J]. J Vet Med Sci, 2006; 68(7)∶ 761.
6.  程湘, 程天民. CD44分子的結構特征[J]. 國外醫(yī)學·免疫學分冊, 1998; 21 (4)∶ 185.
7.  Alho AM, Underhill CB. The hyaluronate receptor is preferentially expressed on proliferating epithelial cells [J]. J Cell Biol, 1989; 108(4)∶ 1557.
8.  夏國偉, 史海安, 周亞魁. 表皮生長因子、谷氨酰胺強化的全胃腸外營養(yǎng)對腸屏障功能和腸細菌移位的影響 [J]. 上海醫(yī)科大學學報, 2000; 27(6)∶ 443.
9.  劉宏, 吳雄飛, 李斂. 大鼠缺血再灌注損傷后腎組織ICAM-1表達及炎細胞浸潤[J]. 第三軍醫(yī)大學學報, 2005; 27(15)∶ 1618.
10.  劉敏豐, 高志光, 秦環(huán)龍. 生長激素和谷氨酰胺對膿毒癥大鼠細胞因子和氨基酸代謝的影響 [J]. 中國普外基礎與臨床雜志, 2006; 13(5)∶ 535.
11.  宮路路, 韓喜春, 姜偉棟. 谷氨酰胺對梗阻性黃疸大鼠可溶性白細胞介素2受體的影響 [J]. 中國普外基礎與臨床雜志, 2006; 13(4)∶ 445.
12.  程雷, 譚廣, 王忠裕, 等. 谷氨酰胺對阻塞性黃疸大鼠免疫功能的影響 [J]. 中國普外基礎與臨床雜志, 2006; 13(1)∶ 71.
13.  Afify A, Pang L, Howell L. Diagnostic utility of CD44 standard, CD44v6, and CD44v3-10 expression in adenocarcinomas presenting in serous fluids[J]. Appl Immunohistochem Mol Morphol, 2007; 15(4)∶ 446.
14.  Cenesiz S, Devrim AK, Kamber U, et al. The effect of kefir on glutathione (GSH), malondialdehyde (MDA) and nitric oxide (NO) levels in mice with colonic abnormal crypt formation (ACF) induced by azoxymethane (AOM) [J]. Dtsch Tierarztl Wochenschr, 2008; 115(1)∶15.
15.  Ito T, Ohi S, Tachibana T, et al. Development of the mucosal vascular system in the distal colon of the fetal mouse [J]. Anat Rec (Hoboken), 2008; 291(1)∶65.
16.  Guebel DV, Torres NV. A computer model of oxygen dynamics in human colon mucosa: implications in normal physiology and early tumor development [J]. J Theor Biol, 2008; 250(3)∶389.
17.  Cheung DY, Kim JI, Park YB, et al. Prolapsing mucosal polyps in the sigmoid colon: presenting with chronic abdominal cramping pain and colonic obstruction [J]. Intern Med, 2007; 46(20)∶1701.
18.  Wang TD, Friedland S, Sahbaie P, et al. Functional imaging of colonic mucosa with a fibered confocal microscope for real-time in vivo pathology [J]. Clin Gastroenterol Hepatol, 2007; 5(11)∶1300.
19.  Spek CA, ten Kate FJ, te Velde AA. Factor V Leiden and the etiology of inflammatory bowel disease [J]. Thromb Haemost, 2007; 98(3)∶670.
20.  Matusiak D, Benya RV. CYP27A1 and CYP24 expression as a function of malignant transformation in the colon [J]. J Histochem Cytochem, 2007; 55(12)∶1257.
21.  Zhaorigetu S, Sasaki M, Kato N. Consumption of sericin suppresses colon oxidative stress and aberrant crypt foci in 1, 2-dimethylhydrazine-treated rats by colon undigested sericin [J]. J Nutr Sci Vitaminol (Tokyo), 2007; 53(3)∶297.
  1. 1.  劉斌, 郝志英. 谷氨酰胺在腫瘤治療中的應用進展[J]. 腫瘤研究與臨床, 2006; 18(3)∶ 207.
  2. 2.  程蕾, 來茂德. 一種新的結直腸癌癌前病變—畸形腺窩灶[J]. 臨床與實驗病理學雜志, 2001; 17(3)∶ 253.
  3. 3.  Araki K, Kobayashi M, Ogata T. A scanning electron microscopic study of the three-dimensional configuration of isolated crypts from human colon adenomas [J]. Med Electron Microsc, 1994; 27(1)∶ 55.
  4. 4.  Inoué T, Osatake H. A new drying method of biological specimens for scanning electron microscopy: the t-butyl alcohol freeze-drying method [J]. Arch Histol Cytol, 1988; 51(1)∶ 53.
  5. 5.  Moon C, Jeong CW, Kim H, et al. Expression of CD44 adhesion molecule in rat testis with ischemia/reperfusion injury [J]. J Vet Med Sci, 2006; 68(7)∶ 761.
  6. 6.  程湘, 程天民. CD44分子的結構特征[J]. 國外醫(yī)學·免疫學分冊, 1998; 21 (4)∶ 185.
  7. 7.  Alho AM, Underhill CB. The hyaluronate receptor is preferentially expressed on proliferating epithelial cells [J]. J Cell Biol, 1989; 108(4)∶ 1557.
  8. 8.  夏國偉, 史海安, 周亞魁. 表皮生長因子、谷氨酰胺強化的全胃腸外營養(yǎng)對腸屏障功能和腸細菌移位的影響 [J]. 上海醫(yī)科大學學報, 2000; 27(6)∶ 443.
  9. 9.  劉宏, 吳雄飛, 李斂. 大鼠缺血再灌注損傷后腎組織ICAM-1表達及炎細胞浸潤[J]. 第三軍醫(yī)大學學報, 2005; 27(15)∶ 1618.
  10. 10.  劉敏豐, 高志光, 秦環(huán)龍. 生長激素和谷氨酰胺對膿毒癥大鼠細胞因子和氨基酸代謝的影響 [J]. 中國普外基礎與臨床雜志, 2006; 13(5)∶ 535.
  11. 11.  宮路路, 韓喜春, 姜偉棟. 谷氨酰胺對梗阻性黃疸大鼠可溶性白細胞介素2受體的影響 [J]. 中國普外基礎與臨床雜志, 2006; 13(4)∶ 445.
  12. 12.  程雷, 譚廣, 王忠裕, 等. 谷氨酰胺對阻塞性黃疸大鼠免疫功能的影響 [J]. 中國普外基礎與臨床雜志, 2006; 13(1)∶ 71.
  13. 13.  Afify A, Pang L, Howell L. Diagnostic utility of CD44 standard, CD44v6, and CD44v3-10 expression in adenocarcinomas presenting in serous fluids[J]. Appl Immunohistochem Mol Morphol, 2007; 15(4)∶ 446.
  14. 14.  Cenesiz S, Devrim AK, Kamber U, et al. The effect of kefir on glutathione (GSH), malondialdehyde (MDA) and nitric oxide (NO) levels in mice with colonic abnormal crypt formation (ACF) induced by azoxymethane (AOM) [J]. Dtsch Tierarztl Wochenschr, 2008; 115(1)∶15.
  15. 15.  Ito T, Ohi S, Tachibana T, et al. Development of the mucosal vascular system in the distal colon of the fetal mouse [J]. Anat Rec (Hoboken), 2008; 291(1)∶65.
  16. 16.  Guebel DV, Torres NV. A computer model of oxygen dynamics in human colon mucosa: implications in normal physiology and early tumor development [J]. J Theor Biol, 2008; 250(3)∶389.
  17. 17.  Cheung DY, Kim JI, Park YB, et al. Prolapsing mucosal polyps in the sigmoid colon: presenting with chronic abdominal cramping pain and colonic obstruction [J]. Intern Med, 2007; 46(20)∶1701.
  18. 18.  Wang TD, Friedland S, Sahbaie P, et al. Functional imaging of colonic mucosa with a fibered confocal microscope for real-time in vivo pathology [J]. Clin Gastroenterol Hepatol, 2007; 5(11)∶1300.
  19. 19.  Spek CA, ten Kate FJ, te Velde AA. Factor V Leiden and the etiology of inflammatory bowel disease [J]. Thromb Haemost, 2007; 98(3)∶670.
  20. 20.  Matusiak D, Benya RV. CYP27A1 and CYP24 expression as a function of malignant transformation in the colon [J]. J Histochem Cytochem, 2007; 55(12)∶1257.
  21. 21.  Zhaorigetu S, Sasaki M, Kato N. Consumption of sericin suppresses colon oxidative stress and aberrant crypt foci in 1, 2-dimethylhydrazine-treated rats by colon undigested sericin [J]. J Nutr Sci Vitaminol (Tokyo), 2007; 53(3)∶297.