目的 通過WST-8和膠原凝膠微滴腫瘤藥物敏感性檢測(CD-DST) 2種藥敏檢測方法的比較，對CD-DST法進(jìn)行評價(jià)。方法 以胰腺癌細(xì)胞系SW1990、PCT-3和ASPC-1為對象，分別采用WST-8和CD-DST法測定其對5-氟尿嘧啶（5-FU）、健擇（GEM）和奧沙利鉑（OXA）3種藥物的敏感性。結(jié)果 在一定的活細(xì)胞數(shù)目范圍內(nèi)（500~10 000個(gè)），CD-DST法所測得的細(xì)胞積分光密度與細(xì)胞數(shù)目成線性正相關(guān)（r=0.991 1, P＜0.05）； 3種腫瘤細(xì)胞的生長抑制率CD-DST法均高于WST-8法(P＜0.05)，2種方法測定的5-FU、GEM和OXA藥物敏感性結(jié)果一致。結(jié)論 CD-DST法可用于胰腺癌的體外藥敏檢測，具有一定的臨床應(yīng)用價(jià)值。
引用本文： 周濤,胡亞,廖泉,趙玉沛. 2種體外腫瘤細(xì)胞藥敏檢測方法的比較研究. 中國普外基礎(chǔ)與臨床雜志, 2008, 15(11): 825-828. doi: 復(fù)制
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- 3. Kobayashi H, Higashiyama M, Minamigawa K, et al. Examination of in vitro chemosensitivity test using collagen gel droplet culture method with colorimetric endpoint quantification [J]. Jpn J Cancer Res, 2001; 92(2)∶203.
- 4. Alley MC, Scudiero DA, Monks A, et al. Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay [J]. Cancer Res, 1988; 48(3)∶589.
- 5. Yang J, Richards J, Bowman P, et al. Sustained growth and three-dimensional organization of primary mammary tumor epithelial cells embedded in collagen gels [J]. Proc Natl Acad Sci USA, 1979; 76(7)∶3401.
- 6. Richards J, Larson L, Yang J, et al. Method for culturing mammary epithelial cells in a rat tail collagen gel matrix [J]. Methods Cell Sci, 1983; 8(1)∶ 31.
- 7. Kawamura M, Gika M, Abiko T, et al. Clinical evaluation of chemosensitivity testing for patients with unresectable non-small cell lung cancer (NSCLC) using collagen gel droplet embedded culture drug sensitivity test (CD-DST) [J]. Cancer Chemother Pharmacol, 2007; 59(4)∶507.
- 8. Vaccarello L, Wang YL, Whiteside TL. Sustained outgrowth of autotumor-reactive T lymphocytes from human ovarian carcinomas in the presence of tumor necrosis factor alpha and interleukin 2 [J]. Hum Immunol, 1990; 28(2)∶216.
- 9. Sieuwerts AM, Klijn JG, Peters HA, et al. The MTT tetrazolium salt assay scrutinized: how to use this assay reliably to measure metabolic activity of cell cultures in vitro for the assessment of growth characteristics, IC50-values and cell survival [J]. Eur J Clin Chem Clin Biochem, 1995; 33(11)∶813.
- 10. Fricker SP, Buckley RG. Comparison of two colorimetric assays as cytotoxicity endpoints for an in vitro screen for antitumour agents [J]. Anticancer Res, 1996; 16(6B)∶3755.
- 11. Chen CF, Hwang JM, Wu CH, et al. Evaluation of a rapid tetrazolium-based colorimetric assay for selecting anticancer drugs [J]. Zhonghua Yi Xue Za Zhi (Taipei), 1990; 46(1)∶7.