• 1成都市第三人民醫(yī)院眼科,四川成都 610031;2四川大學(xué)華西醫(yī)院眼科, 四川成都 610041;

目的:探討重組人IGF-1和生長抑素-14單獨(dú)及聯(lián)合應(yīng)用對甲狀腺相關(guān)眼病患者眼眶成纖維細(xì)胞增殖的影響。方法:體外培養(yǎng)甲狀腺相關(guān)眼病患者眼眶成纖維細(xì)胞,采用四甲基偶氮唑鹽(MTT)比色法檢測不同濃度及作用時間的重組人IGF-1和生長抑素-14對細(xì)胞增殖的作用。結(jié)果:50μg/L以上濃度IGF-1對眼眶成纖維細(xì)胞有促增殖作用,5nmol/L以上濃度的SST對眼眶成纖維細(xì)胞的生長有抑制作用,均呈量效和時效關(guān)系。IGF-1和SST聯(lián)合應(yīng)用時, 50nmol/L以上的SST能阻斷100μg/L的IGF-1促細(xì)胞增殖的作用。結(jié)論:IGF-1可刺激眼眶成纖維細(xì)胞增殖。SST能抑制眼眶成纖維細(xì)胞增殖,并能阻斷IGF-1促進(jìn)細(xì)胞增殖的作用,此抑制作用與濃度有關(guān),提示SST能直接通過受體后機(jī)制抑制IGF-1誘導(dǎo)的眼眶成纖維細(xì)胞增殖。

引用本文： 楊俊峰,羅清禮,張學(xué)進(jìn),何為民,郭波. IGF-1和生長抑素-14對甲狀腺相關(guān)眼病患者眼眶成纖維細(xì)胞增殖的影響. 華西醫(yī)學(xué), 2009, 24(8): 1931-1934. doi: 復(fù)制

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  1. 1. 李志杰.Graves眼病的免疫學(xué)[J].眼科新進(jìn)展,2001,21(5):309-312.
  2. 2. KAZIM M, GOLDBERG RA, SMITH TJ. Insights into the pathogenesis of thyroidassociated orbitopathy[J].Arch Ophthalmol,2002,120(3):380-386.
  3. 3. 陳灝珠.實(shí)用內(nèi)科學(xué)[M].上冊.第10版.北京:人民衛(wèi)生出版社,1998:1024-1026.
  4. 4. 王俊宏.胰島素樣生長因子Ⅰ受體與細(xì)胞凋亡信號轉(zhuǎn)導(dǎo)[J].國外醫(yī)學(xué)·生理、病理科學(xué)與臨床分冊,2002,22(5):445-447.
  5. 5. 王宇泓.IGF-I的免疫調(diào)節(jié)作用[J].國外醫(yī)學(xué)·免疫學(xué)分冊,2001,24(4):187-190.
  6. 6. 顧明君,劉志民,朱莉,等.奧曲肽和潑尼松治療甲狀腺相關(guān)性眼病的隨機(jī)對照研究[J].第二軍醫(yī)大學(xué)學(xué)報,2002,23(5):540-543.
  7. 7. 黃勤,張家慶.Octreotid對人眼球后和皮膚成纖維細(xì)胞DNA合成的影響[J].第二軍醫(yī)大學(xué)學(xué)報,1995,16(6):572-574.
  8. 8. PASQUALI D, VASSALLO P, ESPOSITO D,et al. Somatostatin recptors gene expression and inhibitory effects of octreotide on primary cultures of orbital fibroblasts from Graves’ophthalmopathy[J].J mol Endocrinol,2000,25: 63-71.
  9. 9. 安振梅,魏松全,許淑惠,等.生長抑素對人眼球后成纖維細(xì)胞增殖的影響[J].華西醫(yī)科大學(xué)學(xué)報,2001,32(3):399-401.
  10. 10. KRASSAS GE,PONTIKIDES N,KALTSAS,et al. Free and total insulinlike growth factor(IGF)I,II,and IGF binding protein1,2,and 3 serum levels in patients with active thyroid eye disease[J].J Clin Endocrinol Metab,2003,88(1):132-135.
  11. 11. 何鳳屏,秦雪,李山,等.健康人群的TH與IGF1、DHEA關(guān)系的研究[J].廣西醫(yī)科大學(xué)學(xué)報,2002,19(4):520-522.
  12. 12. JONES JI, CLEMMONS DR. Insulinlike growth factors and their binding proteins: biological actions[J]. Endocr Rev,1995,16(1):3-34.
  13. 13. KLISOVIC DD, SALL JW, O’DORISIO MS,et al.Somatostatin (SST) and octreotide (OCT) inhibit IGF1 mediated tyrosine autophosphorylation of IGF1Rbeta and induction of VEGF gene expression in human RPE cells[J].Invest Ophthalmol Vis Sci,2001,42:S244.