• 四川大學(xué)1.華西醫(yī)院腹部腫瘤科;2.國家重點(diǎn)腫瘤生物治療實(shí)驗(yàn)室,四川成都 610041;

目的:探討人凋亡相關(guān)新基因PNAS-4(hPNAS-4)基因通過脂質(zhì)體轉(zhuǎn)染至Lewis肺癌LL2細(xì)胞后對(duì)放射治療的增敏作用?！糎TH〗方法〖HTSS〗:用脂質(zhì)體介導(dǎo)的轉(zhuǎn)染技術(shù),將hPNAS-4基因?qū)隠ewis肺癌LL2細(xì)胞中,Western blot鑒定其過表達(dá)后,觀察X射線照射對(duì)其集落形成的影響;流式細(xì)胞儀檢測(cè)hPNAS-4基因或／和放療(0,1,2,4,6 Gy)對(duì)LL2細(xì)胞生長抑制及凋亡的影響?！糎TH〗結(jié)果〖HTSS〗:通過Western blot證實(shí)了hPNAS-4基因在LL2細(xì)胞中的過表達(dá)。Lip-hPNAS-4加放射治療處理組細(xì)胞的生存能力明顯降低,腫瘤細(xì)胞的克隆形成明顯減少,流式細(xì)胞術(shù)檢測(cè)體外培養(yǎng)的腫瘤細(xì)胞凋亡明顯增加?！糎TH〗結(jié)論〖HTSS〗:hPNAS-4基因聯(lián)合放射治療能產(chǎn)生協(xié)同抗腫瘤效應(yīng)。

引用本文： 曾輝,石華山,李志平. 人凋亡相關(guān)新基因PNAS-4與放射聯(lián)合治療Lewis肺癌的實(shí)驗(yàn)研究. 華西醫(yī)學(xué), 2009, 24(4): 926-929. doi: 復(fù)制

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17. WEI M C, ZONG W X, CHNEG E H, et al. Proapoptotic BAX and BAK: a requisite gateway to mitochondrial dysfunction and death[J]. Science,2001,292:727-730..
18. HAIMOVITZ-FRIEDMAN A, KAN C C, EHLEITER D. Ionizing radiation acts on cellular membranes to generate ceramide and initiate apoptosis[J]. J Exp Med,1994,180:525-535..
19. HAIMOVITZ-FRIEDMAN A, KAN C C, EHLEITER D. Ionizing radiation acts on cellular membranes to generate ceramide and initiate apoptosis[J]. J Exp Med,1994,180:525-535..
20. ZHIVOTOVSKY B, JOSEPH B, ORRENIUS S. Tumor radiosensitivity and apoptosis[J]. Exp Cell Res,1999,248:10-17..
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22. LI S, YU B, AN P, et al. Combined liposome-mediated cytosine deaminase gene therapy with radiation in killing rectal cancer cells and xenografts in athymic mice[J]. Clin Cancer Res,2005,11(9):3574-3578.[3]LI J H, LAX S A, KIM J, et al. The effects of ionizing radiation and adenoviral p53 therapy in nasopharyngeal carcinoma[J]. Int J Radiat Oncol Biol Phys,1999,43:607-616..
23. LI S, YU B, AN P, et al. Combined liposome-mediated cytosine deaminase gene therapy with radiation in killing rectal cancer cells and xenografts in athymic mice[J]. Clin Cancer Res,2005,11(9):3574-3578.
  1. 1. HUH J J, WOLF J K, FIGHTMASTER D L, et al. Transduction of adenovirus-mediated wild-type p53 after radiotherapy in human cervical cancer cells[J]. Gynecol Oncol,2003,89:243-250..
  2. 2. SAH N K, MUNSHI A, NISHIKAWA T, et al. Adenovirus-mediated wild-type p53 radiasensitizes human tumor cells by suppressing DNA repair capacity[J]. Mol Cancer Ther,2003,2:1223-1231.
  3. 3. LI J H, LAX S A, KIM J, et al. The effects of ionizing radiation and adenoviral p53 therapy in nasopharyngeal carcinoma[J]. Int J Radiat Oncol Biol Phys,1999,43:607-616..
  4. 4. STRAUBERG R L, FEINGOLD E A, GROUSE L H, et al. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences[J]. Proc Natl Acad Sci USA,2002,99(26):16899-16903..
  5. 5. STRAUBERG R L, FEINGOLD E A, GROUSE L H, et al. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences[J]. Proc Natl Acad Sci USA,2002,99(26):16899-16903..
  6. 6. FILIPPOV V, FILIPPOVA M, SINHA D, et al. PNAS-4: a novel pro-apoptotic gene activated during the early response to DNA damage[J]. Proc Am Assoc Cancer Res,2005,46:717(Abstr.3050)..
  7. 7. FILIPPOV V, FILIPPOVA M, SINHA D, et al. PNAS-4: a novel pro-apoptotic gene activated during the early response to DNA damage[J]. Proc Am Assoc Cancer Res,2005,46:717(Abstr.3050)..
  8. 8. YANG F(楊帆), LI Z Y, DENG H X, et al. Efficient inhibition of ovarian cancer growth and prolonged survival by transfection with a novel pro-apoptotic gene, hPNAS-4, in a mouse model. I(xiàn)n vivo and in vitro results[J]. Oncology,2008,75(3-4):137-144..
  9. 9. YANG F(楊帆), LI Z Y, DENG H X, et al. Efficient inhibition of ovarian cancer growth and prolonged survival by transfection with a novel pro-apoptotic gene, hPNAS-4, in a mouse model. I(xiàn)n vivo and in vitro results[J]. Oncology,2008,75(3-4):137-144..
  10. 10. WEI Y Q, ZHAO X, KARIYA Y, et al. Induction of apoptosis by quercetin: involvement of heat shock protein[J]. Cancer Res,1994,54:4952-4957..
  11. 11. WEI Y Q, ZHAO X, KARIYA Y, et al. Induction of apoptosis by quercetin: involvement of heat shock protein[J]. Cancer Res,1994,54:4952-4957..
  12. 12. MAUCERI H J, SEUNG L P, GRDINA W L, et al. Increased injection number enhances adenoviral genetic radiotherapy[J]. Radiat Oncol Invest,1997,5(5):220-226..
  13. 13. MAUCERI H J, SEUNG L P, GRDINA W L, et al. Increased injection number enhances adenoviral genetic radiotherapy[J]. Radiat Oncol Invest,1997,5(5):220-226..
  14. 14. NAKANO K, VOUSDEN K H. PUMA, a novel proapoptotic gene, is induced by P53[J]. Mol Cell,2001,7:683-694.
  15. 15. NAKANO K, VOUSDEN K H. PUMA, a novel proapoptotic gene, is induced by P53[J]. Mol Cell,2001,7:683-694..
  16. 16. WEI M C, ZONG W X, CHNEG E H, et al. Proapoptotic BAX and BAK: a requisite gateway to mitochondrial dysfunction and death[J]. Science,2001,292:727-730..
  17. 17. WEI M C, ZONG W X, CHNEG E H, et al. Proapoptotic BAX and BAK: a requisite gateway to mitochondrial dysfunction and death[J]. Science,2001,292:727-730..
  18. 18. HAIMOVITZ-FRIEDMAN A, KAN C C, EHLEITER D. Ionizing radiation acts on cellular membranes to generate ceramide and initiate apoptosis[J]. J Exp Med,1994,180:525-535..
  19. 19. HAIMOVITZ-FRIEDMAN A, KAN C C, EHLEITER D. Ionizing radiation acts on cellular membranes to generate ceramide and initiate apoptosis[J]. J Exp Med,1994,180:525-535..
  20. 20. ZHIVOTOVSKY B, JOSEPH B, ORRENIUS S. Tumor radiosensitivity and apoptosis[J]. Exp Cell Res,1999,248:10-17..
  21. 21. ZHIVOTOVSKY B, JOSEPH B, ORRENIUS S. Tumor radiosensitivity and apoptosis[J]. Exp Cell Res,1999,248:10-17..
  22. 22. LI S, YU B, AN P, et al. Combined liposome-mediated cytosine deaminase gene therapy with radiation in killing rectal cancer cells and xenografts in athymic mice[J]. Clin Cancer Res,2005,11(9):3574-3578.[3]LI J H, LAX S A, KIM J, et al. The effects of ionizing radiation and adenoviral p53 therapy in nasopharyngeal carcinoma[J]. Int J Radiat Oncol Biol Phys,1999,43:607-616..
  23. 23. LI S, YU B, AN P, et al. Combined liposome-mediated cytosine deaminase gene therapy with radiation in killing rectal cancer cells and xenografts in athymic mice[J]. Clin Cancer Res,2005,11(9):3574-3578.