• 1 西安交通大學(xué)醫(yī)學(xué)院第一附屬醫(yī)院耳鼻喉科（西安，710061）;2 解放軍451醫(yī)院耳鼻喉科;

【摘要】　目的　 探討喉癌組織中 EGFL7基因 mRNA表達(dá)與喉癌分化、轉(zhuǎn)移及臨床預(yù)后的關(guān)系。　方法　 收集2008年5—11月共42例行喉癌手術(shù)患者的切除標(biāo)本。用免疫組織化學(xué)法檢測(cè)EGFL7蛋白在42例喉癌組織和配對(duì)癌旁組織中的表達(dá)情況，提取腫瘤及癌旁組織配對(duì)標(biāo)本的總 RNA，用逆轉(zhuǎn)錄（RT）-PCR方法測(cè)定EGFL7基因表達(dá)，蛋白質(zhì)印跡法測(cè)定EGFL7蛋白的表達(dá)，并結(jié)合臨床資料，對(duì) EGFL7基因差異表達(dá)與喉癌患者臨床表現(xiàn)的相關(guān)性進(jìn)行分析研究。　結(jié)果　 對(duì)42例配對(duì)標(biāo)本分別進(jìn)行 EGFL7 mRNA熒光檢測(cè)比較，30例標(biāo)本的腫瘤組織中EGFL7基因mRNA表達(dá)明顯高于癌旁正常喉組織。22例標(biāo)本的腫瘤組織中EGFL7蛋白表達(dá)明顯高于癌旁正常喉組織。EGFL7 mRNA的表達(dá)與喉癌淋巴結(jié)轉(zhuǎn)移和浸潤(rùn)深度密切相關(guān)（P lt;0.05），而與患者的性別、年齡、吸煙、腫瘤分化程度等無關(guān)（P gt;0.05）。　結(jié)論　 EGFL7基因在喉癌組織中表達(dá)狀態(tài)與喉癌的生長(zhǎng)和浸潤(rùn)轉(zhuǎn)移關(guān)系密切，EGFL7基因可望作為喉癌病情發(fā)展及指導(dǎo)臨床治療的標(biāo)記物之一。
【Abstract】　Objective　 To investigate the association of EGF-like-domain, multiple 7 (EGFL7) mRNA expression level with the differentiation, metastasis and prognosis of laryngocarcinoma. 　Methods　 Tissue specimens were obtained from 42 patients undergoing surgery for laryngocarcinoma between May and November, 2008. Immunohistochemistry was used to detect the level of EGFL7 in the 42 tumor tissues and matched adjacent normal tissues. Reverse transcriptional PCR (RT-PCR) was performed for amplification of EGFL7 mRNA from the 42 tumor tissues and matched adjacent normal tissues, and westerblot was adopted to determine EGFL7 protein expression. The differential EGFL7 mRNA expression was analyzed for its association with the clinical manifestations of the patients. 　Results　 EGFL7 mRNA expression was detected in all the laryngocarcinoma tissues and normal tissues adjacent to the carcinoma using fluorescence method. EGFL7 mRNA expression was significantly higher in the tumor tissues than in the adjacent tissues in 30 cases, and EGFL7 protein expression was also significantly higher in the tumor tissues than in the adjacent normal laryngeal tissues in 22 cases. Expression of EGFL7 mRNA was highly correlated with lymph node metastasis and T classification (P lt;0.05), but was not correlated with the patients’ gender, age, or tumor differentiation (P gt;0.05). 　Conclusions　 EGFL7 mRNA expression is correlated closely with the differentiation and metastasis of laryngocarcinoma. EGFL7 may serve as a marker for assessing the progression of laryngocarcinoma and provide assistance for clinical therapeutic decisions.

引用本文： 王曉俠,姚小寶,嵇憲生,陳菁華,李蕾,朱宏亮. EGFL7基因過度表達(dá)與喉癌分化、轉(zhuǎn)移及預(yù)后的關(guān)系. 華西醫(yī)學(xué), 2011, 26(5): 710-713. doi: 復(fù)制

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1. 　Chambers AF, Groom AC, MacDonald JC. Dissemination and growth of cancer cells in metastasis sites[J]. Nat Rev Cancer. 2002, 2(8): 563-572.
2. 　Bernards R. Cancer: cue for migration[J]. Nature, 2003, 425(6955): 247-248.
3. 　Campag L, Leahy A, Chitnis S, et al. EGFL7 is a chemo attractant for endothelial cells and is up-regulated in angiogenesis and arterial injury[J]. Am J Pathol, 2005, 167(1): 275-284.
4. 　Park LH, Schmidt M, Jin SW, et al. The endothelial-cell-derived secreted factor EGFL7 regulates vescular tube formation [J]. Nature, 2004, 428: 754-758.
5. 　Parker LH, Schmidt M, Jin SW, et al. The endothelial cell derived secreted factor EGFL7 regulates vascular tube formation[J]. Nature, 2004, 428: 754-758.
6. 　劉志勇, 歐陽忠, 鄒小明. EGFL7基因沉默對(duì)裸鼠胃癌血管生成的影響[J]. 貴陽醫(yī)學(xué)院學(xué)報(bào), 2010, 35(3): 259-263.
7. 　Greene FL, Page DL, Fleming ID, et al. AJCC cancer staging handbook[M]. 6th ed. New York: Springer-Verlag, 2002:61-71.
8. 　韓冰, 趙潔, 周娟. RB基因失活與腫瘤發(fā)生[J]. 菏澤醫(yī)學(xué)?？茖W(xué)校學(xué)報(bào), 2009, 21(2): 67-68.
9. 　Breitman ML, Rossant J. flk-1, an flt-related receptor tyrosine kinase is early marker for endothelial cell precursors[J]. Development, 1993, 118: 489-498.
10. 　薛倩, 周光耀, 鄒劍, 等. 血管內(nèi)皮生長(zhǎng)因子在喉癌組織中的表達(dá)及其意義[J]. 華西醫(yī)學(xué), 2004, 3(19): 366-367.
11. 　Fitch MJ, Campagnolo L, Kuhnert F, et al. EGFl7, a novel epidermal growth factor-domain gene expressed in endothelial cells[J]. Dev Dyn, 2004, 230(2): 316-324.
12. 　李曉晗, 王小松, 韓琤波, 等. 血管生成素與受體和缺氧誘導(dǎo)因子在膠質(zhì)瘤血管新生中的意義[J]. 中華病理學(xué)雜志, 2008, 37(3): 188-189.
13. 　Chatni SS, Thankappan K. Lymph node status cannot be ignored in survival analysis of laryngeal cancer[J]. Arch Otolaryngol Head Neck Surg, 2008, 134(7): 786-787.
14. 　Woodhouse EC, Chuaqui RF, Liotta LA. General mechanisms of metastasis [J]. Cancer, 1997, 80(8): 1529-1537.
15. 　鄭亞民, 達(dá)明緒, 趙紫罡, 等. 胃癌癌周微淋巴管密度與腫瘤侵襲轉(zhuǎn)移的相關(guān)性研究[J]. 華西醫(yī)學(xué), 2006, 21 (2):269-271.
  1. 1. 　Chambers AF, Groom AC, MacDonald JC. Dissemination and growth of cancer cells in metastasis sites[J]. Nat Rev Cancer. 2002, 2(8): 563-572.
  2. 2. 　Bernards R. Cancer: cue for migration[J]. Nature, 2003, 425(6955): 247-248.
  3. 3. 　Campag L, Leahy A, Chitnis S, et al. EGFL7 is a chemo attractant for endothelial cells and is up-regulated in angiogenesis and arterial injury[J]. Am J Pathol, 2005, 167(1): 275-284.
  4. 4. 　Park LH, Schmidt M, Jin SW, et al. The endothelial-cell-derived secreted factor EGFL7 regulates vescular tube formation [J]. Nature, 2004, 428: 754-758.
  5. 5. 　Parker LH, Schmidt M, Jin SW, et al. The endothelial cell derived secreted factor EGFL7 regulates vascular tube formation[J]. Nature, 2004, 428: 754-758.
  6. 6. 　劉志勇, 歐陽忠, 鄒小明. EGFL7基因沉默對(duì)裸鼠胃癌血管生成的影響[J]. 貴陽醫(yī)學(xué)院學(xué)報(bào), 2010, 35(3): 259-263.
  7. 7. 　Greene FL, Page DL, Fleming ID, et al. AJCC cancer staging handbook[M]. 6th ed. New York: Springer-Verlag, 2002:61-71.
  8. 8. 　韓冰, 趙潔, 周娟. RB基因失活與腫瘤發(fā)生[J]. 菏澤醫(yī)學(xué)?？茖W(xué)校學(xué)報(bào), 2009, 21(2): 67-68.
  9. 9. 　Breitman ML, Rossant J. flk-1, an flt-related receptor tyrosine kinase is early marker for endothelial cell precursors[J]. Development, 1993, 118: 489-498.
  10. 10. 　薛倩, 周光耀, 鄒劍, 等. 血管內(nèi)皮生長(zhǎng)因子在喉癌組織中的表達(dá)及其意義[J]. 華西醫(yī)學(xué), 2004, 3(19): 366-367.
  11. 11. 　Fitch MJ, Campagnolo L, Kuhnert F, et al. EGFl7, a novel epidermal growth factor-domain gene expressed in endothelial cells[J]. Dev Dyn, 2004, 230(2): 316-324.
  12. 12. 　李曉晗, 王小松, 韓琤波, 等. 血管生成素與受體和缺氧誘導(dǎo)因子在膠質(zhì)瘤血管新生中的意義[J]. 中華病理學(xué)雜志, 2008, 37(3): 188-189.
  13. 13. 　Chatni SS, Thankappan K. Lymph node status cannot be ignored in survival analysis of laryngeal cancer[J]. Arch Otolaryngol Head Neck Surg, 2008, 134(7): 786-787.
  14. 14. 　Woodhouse EC, Chuaqui RF, Liotta LA. General mechanisms of metastasis [J]. Cancer, 1997, 80(8): 1529-1537.
  15. 15. 　鄭亞民, 達(dá)明緒, 趙紫罡, 等. 胃癌癌周微淋巴管密度與腫瘤侵襲轉(zhuǎn)移的相關(guān)性研究[J]. 華西醫(yī)學(xué), 2006, 21 (2):269-271.