• 1 西安交通大學(xué)醫(yī)學(xué)院第二附屬醫(yī)院骨一科（西安，710004）;;
  • 2 西安交通大學(xué)醫(yī)學(xué)院第三附屬醫(yī)院（陜西省人民醫(yī)院）神經(jīng)外科;

目的 探討脂肪干細(xì)胞（adipose-derived stem cells，ADSCs）及血管束植入法聯(lián)合應(yīng)用對組織工程支
架體內(nèi)血管化的影響，為臨床應(yīng)用組織工程支架復(fù)合物治療股骨頭缺血性壞死提供理論依據(jù)。 方法 取4 月齡SD 大
鼠，分離培養(yǎng)ADSCs，并行成骨誘導(dǎo)鑒定。取第3 代ADSCs 接種于納米羥基磷灰石/ 聚酰胺66（nano-hydroxyapatide/
polyamide-66，nHA/PA66）支架上制備復(fù)合支架，掃描電鏡觀察細(xì)胞與支架復(fù)合情況。取4 月齡SD 大鼠24 只，體重
350 ～ 400 g，隨機(jī)分為3 組，每組8 只。A、B 組分離大鼠雙側(cè)腹壁下動、靜脈，分別將血管束植入培養(yǎng)10 d 的復(fù)合支架及
單純nHA/PA66 支架；C 組將培養(yǎng)10 d 的復(fù)合支架包埋入雙側(cè)股四頭肌中。術(shù)后2、4 周每組取4 只大鼠支架標(biāo)本行HE
染色及CD34 免疫組織化學(xué)染色觀察，檢測其血管生成情況。 結(jié)果 所分離細(xì)胞經(jīng)鑒定為ADSCs。掃描電鏡觀察示復(fù)
合培養(yǎng)10 d，細(xì)胞數(shù)目增多，形態(tài)完全伸展，呈長梭形。術(shù)后2、4 周，HE 染色及免疫組織化學(xué)染色均顯示A 組支架植入動、
靜脈周圍有大量血管生成，血管數(shù)量及管壁成熟度均優(yōu)于同一時間點(diǎn)的B、C 組。術(shù)后2、4 周，A 組血管密度和血管直徑
均顯著大于B、C 組，B 組大于C 組，差異均有統(tǒng)計(jì)學(xué)意義（P  lt; 0.05）。 結(jié)論 ADSCs 和血管束植入法聯(lián)合應(yīng)用可以促
進(jìn)組織工程支架體內(nèi)血管化程度。

引用本文： 姬文晨,楊佩,張?jiān)搅?王春生,倪建龍,張永濤,王坤正. 脂肪干細(xì)胞及血管束植入法聯(lián)合應(yīng)用對組織工程支架體內(nèi)血管化的影響. 中國修復(fù)重建外科雜志, 2012, 26(2): 129-134. doi: 復(fù)制

1. Mont MA, Jones LC, Hungerford DS, et al. Nontraumatic osteonecrosis of the femoral head: ten years later. J Bone Joint Surg (Am), 2006, 88(5): 1117-1132.
2. Wen Q, Ma L, Chen YP, et al. Treatment of avascular necrosis of the femoral head by hepatocyte growth factor-transgenic bone marrow stromal stem cells. Gene Ther, 2008, 15(23): 1523-1535.
3. Picinich SC, Mishra PJ, Mishra PJ, et al. The therapeutic potential of mesenchymal stem cell. Cell-tissue-based therapy. Expert Opin Biol Ther, 2007, 7(7): 965-973.
4. Kalita SJ, Bhardwaj A, Bhatt HA. Nanocrystalline calcium phosphate ceramics in biomeical engineering. Materials Science and Engineering, 2007, 27(3): 441-449.
5. 趙文, 江昭林, 魏朝陽, 等. 人工椎板(納米羥基磷灰石/聚酰胺66)的臨床應(yīng)用. 臨床骨科雜志, 2009, 12(4): 398-399.
6. Cai X, Tong H, Shen X, et al. Preparation and characterization of homogeneous chitosan-polylactic acid/hydroxyapatite nanocomposite for bone tissue engineering and evaluation of its mechanical properties. Acta Biomater, 2009, 5(7): 2693-2703.
7. 戚孟春, 鄧久鵬, 董偉, 等. 骨髓間充質(zhì)干細(xì)胞與支架材料nHA/PA66復(fù)合培養(yǎng)的生物活性研究. 口腔醫(yī)學(xué)研究, 2010, 26(6): 805-808.
8. Doldercr JH, Abberton KM, Thompson EW, et al. Spontaneous large volume adipose tissue generation from a vascularized pedicled fat flap inside a chamber space. Tissue Eng, 2007, 13(4): 673-681.
9. Pelissier P, Villars F, Mathoulin-Pelissier S, et al. Influences of vascularization and osteogenic cells on heterotopic bone formation within a madreporic ceramic in rats. Plast Reconstr Surg, 2003, 111(6): 1932-1941.
10. 王簕, 裴國獻(xiàn), 高梁斌, 等. 血管化組織工程骨修復(fù)兔股骨干骨缺損. 中華醫(yī)學(xué)雜志, 2010, 90(23): 1637-1641.
11. 王簕, 覃俊君, 裴國獻(xiàn), 等. 血管束植入組織工程骨修復(fù)兔股骨缺損對血管內(nèi)皮生長因子表達(dá)的影響. 中華創(chuàng)傷骨科雜志, 2009, 11(6): 540-545.
12. Li B, Zeng Q, Wang H, et al. Effect of cytokines secreted by human adipose stromal cells on endothelial cells. J Huazhong Univ Sci Technolog Med Sci, 2006, 26(4): 396-398.
13. Sadat S, Gehmert S, Song YH, et al. The cardioprotective effect of stem cells is mediated by IGF-I and VEGF. Biochem Biophys Res Commun, 2007, 363(3): 674-679.
14. Shigemura N, Ukumura M, Mizuno S, et al. Autologous transplantation of adipose tissue-derived stromal cells ameliorates pulmonary emphysema. Am J Transplant, 2006, 6(11): 2592-2600.
15. Lu F, Mizuno H, Uysal CA, et al. Improved viability of random pattern skin flaps through the use of adipose-derived stem cells. Plast Reconstr Surg, 2008, 121(1): 50-58.
16. Suga H, Shigeura T, Matsumoto D, et al. Rapid expansion of human adipose-derived stromal cells preserving multipotency. Cytotherapy, 2007, 9(8): 738-745.
17. De Francesco F, Tirino V, Desiderio V, et al. Human CD34+/CD90+ ASCs are capable of growing as sphere clusters, producing high levels of VEGF and forming capillaries. PLoS One, 2009, 4(8): e6537.
18. Rehman J, Traktuev D, Li J, et al. Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells. Circulation, 2004, 109(10): 1292-1298.
19. Sanz-Ruiz R, Santos ME, Muñoa MD, et al. Adipose tissue-derived stem cells: the friendly side of a classic cardiovascular foe. J Cardiovasc Transl Res, 2008, 1(1): 55-63.
20. Zuk PA, Zhu M, Mizuno H, et al. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng, 2001, 7(2): 211-228.
21. Jurgens WJ, Oedayrajsingh-Varma MJ, Helder MN, et al. Effect of tissue-harvesting site on yield of stem cells derived from adipose tissue: implications for cell-based therapies. Cell Tissue Res, 2008, 332(3): 415-426.
  1. 1. Mont MA, Jones LC, Hungerford DS, et al. Nontraumatic osteonecrosis of the femoral head: ten years later. J Bone Joint Surg (Am), 2006, 88(5): 1117-1132.
  2. 2. Wen Q, Ma L, Chen YP, et al. Treatment of avascular necrosis of the femoral head by hepatocyte growth factor-transgenic bone marrow stromal stem cells. Gene Ther, 2008, 15(23): 1523-1535.
  3. 3. Picinich SC, Mishra PJ, Mishra PJ, et al. The therapeutic potential of mesenchymal stem cell. Cell-tissue-based therapy. Expert Opin Biol Ther, 2007, 7(7): 965-973.
  4. 4. Kalita SJ, Bhardwaj A, Bhatt HA. Nanocrystalline calcium phosphate ceramics in biomeical engineering. Materials Science and Engineering, 2007, 27(3): 441-449.
  5. 5. 趙文, 江昭林, 魏朝陽, 等. 人工椎板(納米羥基磷灰石/聚酰胺66)的臨床應(yīng)用. 臨床骨科雜志, 2009, 12(4): 398-399.
  6. 6. Cai X, Tong H, Shen X, et al. Preparation and characterization of homogeneous chitosan-polylactic acid/hydroxyapatite nanocomposite for bone tissue engineering and evaluation of its mechanical properties. Acta Biomater, 2009, 5(7): 2693-2703.
  7. 7. 戚孟春, 鄧久鵬, 董偉, 等. 骨髓間充質(zhì)干細(xì)胞與支架材料nHA/PA66復(fù)合培養(yǎng)的生物活性研究. 口腔醫(yī)學(xué)研究, 2010, 26(6): 805-808.
  8. 8. Doldercr JH, Abberton KM, Thompson EW, et al. Spontaneous large volume adipose tissue generation from a vascularized pedicled fat flap inside a chamber space. Tissue Eng, 2007, 13(4): 673-681.
  9. 9. Pelissier P, Villars F, Mathoulin-Pelissier S, et al. Influences of vascularization and osteogenic cells on heterotopic bone formation within a madreporic ceramic in rats. Plast Reconstr Surg, 2003, 111(6): 1932-1941.
  10. 10. 王簕, 裴國獻(xiàn), 高梁斌, 等. 血管化組織工程骨修復(fù)兔股骨干骨缺損. 中華醫(yī)學(xué)雜志, 2010, 90(23): 1637-1641.
  11. 11. 王簕, 覃俊君, 裴國獻(xiàn), 等. 血管束植入組織工程骨修復(fù)兔股骨缺損對血管內(nèi)皮生長因子表達(dá)的影響. 中華創(chuàng)傷骨科雜志, 2009, 11(6): 540-545.
  12. 12. Li B, Zeng Q, Wang H, et al. Effect of cytokines secreted by human adipose stromal cells on endothelial cells. J Huazhong Univ Sci Technolog Med Sci, 2006, 26(4): 396-398.
  13. 13. Sadat S, Gehmert S, Song YH, et al. The cardioprotective effect of stem cells is mediated by IGF-I and VEGF. Biochem Biophys Res Commun, 2007, 363(3): 674-679.
  14. 14. Shigemura N, Ukumura M, Mizuno S, et al. Autologous transplantation of adipose tissue-derived stromal cells ameliorates pulmonary emphysema. Am J Transplant, 2006, 6(11): 2592-2600.
  15. 15. Lu F, Mizuno H, Uysal CA, et al. Improved viability of random pattern skin flaps through the use of adipose-derived stem cells. Plast Reconstr Surg, 2008, 121(1): 50-58.
  16. 16. Suga H, Shigeura T, Matsumoto D, et al. Rapid expansion of human adipose-derived stromal cells preserving multipotency. Cytotherapy, 2007, 9(8): 738-745.
  17. 17. De Francesco F, Tirino V, Desiderio V, et al. Human CD34+/CD90+ ASCs are capable of growing as sphere clusters, producing high levels of VEGF and forming capillaries. PLoS One, 2009, 4(8): e6537.
  18. 18. Rehman J, Traktuev D, Li J, et al. Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells. Circulation, 2004, 109(10): 1292-1298.
  19. 19. Sanz-Ruiz R, Santos ME, Muñoa MD, et al. Adipose tissue-derived stem cells: the friendly side of a classic cardiovascular foe. J Cardiovasc Transl Res, 2008, 1(1): 55-63.
  20. 20. Zuk PA, Zhu M, Mizuno H, et al. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng, 2001, 7(2): 211-228.
  21. 21. Jurgens WJ, Oedayrajsingh-Varma MJ, Helder MN, et al. Effect of tissue-harvesting site on yield of stem cells derived from adipose tissue: implications for cell-based therapies. Cell Tissue Res, 2008, 332(3): 415-426.