目的 通過研究不同吸煙量對大鼠血清瘦素、脂聯(lián)素、IL-6 及C 反應(yīng)蛋白( CRP) 水平的影響, 探討瘦素和脂聯(lián)素等炎癥因子在吸煙所致COPD 全身炎癥反應(yīng)中的作用。方法 雄性Wistar 大鼠30 只, 隨機(jī)分為大量吸煙組, 小量吸煙組和對照組, 每組各10 只。大量吸煙組每次吸煙10 支, 每天吸煙2 次, 每周吸煙6 d, 共吸煙20 周; 小量吸煙組每次吸煙5 支, 吸煙時(shí)間同前。采用雙抗體夾心酶聯(lián)免疫吸附法( ABC-ELISA) 分別檢測血清瘦素、脂聯(lián)素、IL-6 及CRP 水平。結(jié)果 大量吸煙組和小量吸煙組血清瘦素和脂聯(lián)素水平均較對照組降低( P 均 lt;0. 05) , 大量吸煙組與小量吸煙組差異無統(tǒng)計(jì)學(xué)意義( P gt;0. 05) 。大量吸煙組和小量吸煙組血清IL-6 和CRP 水平均較對照組升高( P 均 lt;0. 05) , 大量吸煙組較小量吸煙組水平高( P 均 lt;0. 05) 。結(jié)論 吸煙可使大鼠血清瘦素與脂聯(lián)素水平下降, IL-6 與CRP水平升高, 提示瘦素、脂聯(lián)素等炎癥因子可能參與吸煙所致COPD 的全身炎癥反應(yīng)。
引用本文： 白學(xué)禮,許建英. 吸煙對大鼠血清瘦素、脂聯(lián)素、IL-6 和C 反應(yīng)蛋白的影響. 中國呼吸與危重監(jiān)護(hù)雜志, 2010, 9(3): 298-300. doi: 復(fù)制
| 1. | van Weel C, Schellevis FG. Comorbidity and guidelines: conflicting interests. Lancet, 2006 , 367: 550-551. |
| 2. | Sin DD, Anthonisen NR, Soriano JB, et al. Mortality in COPD: Role of comorbidities. Eur Respir J, 2006, 28: 1245-1257. |
| 3. | Schols AM, Creutzerg EC, Buurman WA, et al. Plasma leptin is related to proinflammatory status and dietary intake in patients with chronic obstructive pulmonary disease. Am J Respir Crit Care Med,1999, 160: 1220-1226. |
| 4. | Kirdar S, Serter M, Ceylan E, et al. Adiponectin as a biomarker of systemic inflammatory response in smoker patients with stable and exacerbation phases of chronic obstructive pulmonary disease. Scand.J Clin Lab Invest, 2009, 69: 219 -224. |
| 5. | 許三林, 吳人亮, 陳春蓮, 等. 上皮鈣黏附素在吸煙小鼠呼吸道上皮損傷修復(fù)中表達(dá)的研究. 中華結(jié)核和呼吸雜志,1999, 22:417-419. |
| 6. | Chen H, Hansen MJ, Jones JE, et al. Long-term cigarette smoke exposure increases uncoupling protein expression but reduces energy intake. Brain Res, 2008, 1228: 81-88. |
| 7. | Hotta Y, Yatsuya H, Toyoshima H, et al. Low leptin but high insulin resistance of smokers in Japanese men. Diabetes Res Clin Pract,2008, 81: 358-364 . |
| 8. | Sull JW, Kim HJ, Yun JE, et al. Serumadiponectin is associated with smoking status in healthy Korean men. Endocr J, 2009, 56: 73-78. |
| 9. | Kirchgessner TG, Uysal KT, Wiesbrock SM, et al. Tumor necrosis factor-alpha contributes to obesity-related hyperleptinemia by regulating leptin release from adipocytes. J Clin Invest, 1997, 100 :2777-2782. |
| 10. | Trujillo ME, Sullivan S, Harten I, et al. Interleukin-6 regulates human adipose tissue lipid metabolism and leptin production in vitro. J Clin Endocrinol Metab, 2004, 89 : 5577 -5582 . |
| 11. | Fantuzzi G. Adipose tissue, adipokines, and inflammation. J Allergy Clin Immunol, 2005, 115: 911 -919. |
| 12. | Gettys TW, Harkness PJ, Waston PM. The β3-aderenergic receptor inhibits insulin-stinulated leptin secretion from isolated rat adipocytes. Endocrinology, 1996, 137: 4054-4057. |
| 13. | Bruun JM, Lihn AS, Verdich C, et al. Regulation of adiponectin by adipose tissue-derived cytokines: in vivo and in vitro investigations in humans. Am J Physiol Endocrinol Metab, 2003, 285 : E527-533. |
| 14. | Iwashima Y, Katauya T, Ishikawa K, et al. Association of hypoadiponectinemia with smoking habit in men. Hypertension,2005, 45: 1094-1100 . |
| 15. | Pepys MB, Hirschfield GM. C-reactive protein: a critical update. J Clin Invest, 2003 , 111: 1805-1812. |
- 1. van Weel C, Schellevis FG. Comorbidity and guidelines: conflicting interests. Lancet, 2006 , 367: 550-551.
- 2. Sin DD, Anthonisen NR, Soriano JB, et al. Mortality in COPD: Role of comorbidities. Eur Respir J, 2006, 28: 1245-1257.
- 3. Schols AM, Creutzerg EC, Buurman WA, et al. Plasma leptin is related to proinflammatory status and dietary intake in patients with chronic obstructive pulmonary disease. Am J Respir Crit Care Med,1999, 160: 1220-1226.
- 4. Kirdar S, Serter M, Ceylan E, et al. Adiponectin as a biomarker of systemic inflammatory response in smoker patients with stable and exacerbation phases of chronic obstructive pulmonary disease. Scand.J Clin Lab Invest, 2009, 69: 219 -224.
- 5. 許三林, 吳人亮, 陳春蓮, 等. 上皮鈣黏附素在吸煙小鼠呼吸道上皮損傷修復(fù)中表達(dá)的研究. 中華結(jié)核和呼吸雜志,1999, 22:417-419.
- 6. Chen H, Hansen MJ, Jones JE, et al. Long-term cigarette smoke exposure increases uncoupling protein expression but reduces energy intake. Brain Res, 2008, 1228: 81-88.
- 7. Hotta Y, Yatsuya H, Toyoshima H, et al. Low leptin but high insulin resistance of smokers in Japanese men. Diabetes Res Clin Pract,2008, 81: 358-364 .
- 8. Sull JW, Kim HJ, Yun JE, et al. Serumadiponectin is associated with smoking status in healthy Korean men. Endocr J, 2009, 56: 73-78.
- 9. Kirchgessner TG, Uysal KT, Wiesbrock SM, et al. Tumor necrosis factor-alpha contributes to obesity-related hyperleptinemia by regulating leptin release from adipocytes. J Clin Invest, 1997, 100 :2777-2782.
- 10. Trujillo ME, Sullivan S, Harten I, et al. Interleukin-6 regulates human adipose tissue lipid metabolism and leptin production in vitro. J Clin Endocrinol Metab, 2004, 89 : 5577 -5582 .
- 11. Fantuzzi G. Adipose tissue, adipokines, and inflammation. J Allergy Clin Immunol, 2005, 115: 911 -919.
- 12. Gettys TW, Harkness PJ, Waston PM. The β3-aderenergic receptor inhibits insulin-stinulated leptin secretion from isolated rat adipocytes. Endocrinology, 1996, 137: 4054-4057.
- 13. Bruun JM, Lihn AS, Verdich C, et al. Regulation of adiponectin by adipose tissue-derived cytokines: in vivo and in vitro investigations in humans. Am J Physiol Endocrinol Metab, 2003, 285 : E527-533.
- 14. Iwashima Y, Katauya T, Ishikawa K, et al. Association of hypoadiponectinemia with smoking habit in men. Hypertension,2005, 45: 1094-1100 .
- 15. Pepys MB, Hirschfield GM. C-reactive protein: a critical update. J Clin Invest, 2003 , 111: 1805-1812.