目的 探討哮喘大鼠肺臟、鼻腔、腸道黏膜免疫的相互關(guān)聯(lián)和變化規(guī)律。方法 將20只Wistar 大鼠分為對(duì)照組和哮喘組, 各10 只。采用卵白蛋白致敏和激發(fā)的方法制作哮喘模型。應(yīng)用免疫組化和原位雜交技術(shù)檢測(cè)大鼠肺組織、鼻黏膜、腸黏膜中的CD4 + 、CD8 + 淋巴細(xì)胞數(shù)量, eotaxin蛋白及其mRNA 表達(dá)。取大鼠支氣管肺泡灌洗液( BALF) 、鼻咽沖洗液、腸黏液上清液, 通過酶聯(lián)免疫吸附試驗(yàn)檢測(cè)sIgA 含量。結(jié)果 哮喘組肺組織、鼻黏膜、腸黏膜中CD4 + 和CD8 + 表達(dá)較對(duì)照組增高( P lt;0. 05) , 肺組織中eotaxin 蛋白及mRNA、鼻咽沖洗液中sIgA、腸黏膜eotaxin 蛋白表達(dá)較對(duì)照組增高( P lt;0. 05) 。其余指標(biāo)兩組間水平無顯著差異。對(duì)照組大鼠eotaxin 蛋白表達(dá)在肺組織和鼻黏膜呈負(fù)相關(guān)( r= - 0. 572, P =0. 008) , 在腸黏膜和鼻黏膜呈正相關(guān)( r =0. 638, P =0. 002) ; eotaxin mRNA表達(dá)在肺組織和鼻黏膜呈正相關(guān)( r =0. 502, P = 0. 024) , 在腸黏膜和鼻黏膜呈正相關(guān)( r =0. 594, P =0. 006) 。在哮喘組, 除了eotaxin 蛋白表達(dá)在肺組織和腸黏膜呈負(fù)相關(guān)( r = - 0. 448, P = 0. 048) , 其余指標(biāo)間無顯著相關(guān)性。結(jié)論 在正常生理狀態(tài)下, 肺、鼻、大腸之間的黏膜免疫功能可能保持密切的關(guān)系或動(dòng)態(tài)平衡。但是在哮喘發(fā)病過程中, 這種平衡被破壞。
引用本文： 李風(fēng)森,秦慧娟,哈木拉提吾甫爾. 哮喘大鼠肺臟、鼻腔、腸道黏膜免疫相互關(guān)聯(lián)的研究. 中國(guó)呼吸與危重監(jiān)護(hù)雜志, 2010, 9(3): 273-275. doi: 復(fù)制
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| 1. | 中華醫(yī)學(xué)會(huì)呼吸病學(xué)分會(huì)哮喘學(xué)組. 支氣管哮喘防治指南( 支氣管哮喘的定義、診斷、治療及教育和管理方案) . 中華內(nèi)科學(xué)雜志, 2003 , 42: 817 -822. |
| 2. | Umetsu DT, DeKruyff RH. The regulation of allergy and asthma.Immunol Rev, 2006 , 212: 238-255 . |
| 3. | Wilson JW, Kotsimbos T. Airway vascular remodeling in asthma.Curr Allergy Asthma Rep, 2003, 3: 153 -158. |
| 4. | 崔龍?zhí)O, 楊永清, 陳漢平, 等. 過敏性哮喘大鼠模型的制備. 上海實(shí)驗(yàn)動(dòng)物科學(xué), 2000, 20: 69 -71 . |
| 5. | Deng YM, Xie QM, Chen JQ, et al. Coincidential increase of leukotriene B4 between cerebral cortex and lung tissue of seneitized rats. Acta Pharmacol Sin, 2003 , 24 : 1039-1044. |
| 6. | Bousquet J, Khaltaev N, Cruz AA, et al. Allergic Rhinitis and its Impact on Asthma( ARIA) 2008 update ( in collaboration with the World Health Organization, GA( 2 ) LEN and AllerGen) . Allergy,2008, 63 Suppl 86: 8-160 . |
| 7. | 高杰英. 黏膜免疫向免疫學(xué)提出了新問題. 上海免疫學(xué)雜志,2000, 20: 257-259 . |
| 8. | Mayer L. Mucosal immunity. Pediatrics, 2003, 111: 1595-1600. |
| 9. | Hideki I, Ichiro T, Hiroshi K. Mucosal immune network in the gut for the control of infectious diseases. Rev Med Virol, 2001, 11: 117 -133. |
| 10. | Makala LHC, Suzuki N, Nagasawa H. Peyer’s patches: organized lymphoid structures for the induction of mucosal immune responsesin the intestine. Pathobiology, 2002, 70 : 55 -68. |
- 1. 中華醫(yī)學(xué)會(huì)呼吸病學(xué)分會(huì)哮喘學(xué)組. 支氣管哮喘防治指南( 支氣管哮喘的定義、診斷、治療及教育和管理方案) . 中華內(nèi)科學(xué)雜志, 2003 , 42: 817 -822.
- 2. Umetsu DT, DeKruyff RH. The regulation of allergy and asthma.Immunol Rev, 2006 , 212: 238-255 .
- 3. Wilson JW, Kotsimbos T. Airway vascular remodeling in asthma.Curr Allergy Asthma Rep, 2003, 3: 153 -158.
- 4. 崔龍?zhí)O, 楊永清, 陳漢平, 等. 過敏性哮喘大鼠模型的制備. 上海實(shí)驗(yàn)動(dòng)物科學(xué), 2000, 20: 69 -71 .
- 5. Deng YM, Xie QM, Chen JQ, et al. Coincidential increase of leukotriene B4 between cerebral cortex and lung tissue of seneitized rats. Acta Pharmacol Sin, 2003 , 24 : 1039-1044.
- 6. Bousquet J, Khaltaev N, Cruz AA, et al. Allergic Rhinitis and its Impact on Asthma( ARIA) 2008 update ( in collaboration with the World Health Organization, GA( 2 ) LEN and AllerGen) . Allergy,2008, 63 Suppl 86: 8-160 .
- 7. 高杰英. 黏膜免疫向免疫學(xué)提出了新問題. 上海免疫學(xué)雜志,2000, 20: 257-259 .
- 8. Mayer L. Mucosal immunity. Pediatrics, 2003, 111: 1595-1600.
- 9. Hideki I, Ichiro T, Hiroshi K. Mucosal immune network in the gut for the control of infectious diseases. Rev Med Virol, 2001, 11: 117 -133.
- 10. Makala LHC, Suzuki N, Nagasawa H. Peyer’s patches: organized lymphoid structures for the induction of mucosal immune responsesin the intestine. Pathobiology, 2002, 70 : 55 -68.