目的 探討自由基清除劑依達(dá)拉奉( ED) 對膿毒癥致ALI 的保護(hù)作用。方法 24 只雄性Wistar 大鼠隨機(jī)分成3 組: 對照組( NS組) 、模型組( LPS 組) 及依達(dá)拉奉治療組( ED 組) 。LPS 組和ED 組采用尾靜脈注射LPS( 10 mg/ kg) 建立ALI 模型, ED 組隨后立即尾靜脈注射依達(dá)拉奉( 3 mg/kg) 。LPS 注射6 h后留取肺標(biāo)本, 分別測定肺組織濕/干重比值( W/D) 和肺組織勻漿中髓過氧化物酶( MPO) 、丙二醛( MDA) 和超氧化物歧化酶( SOD) 含量, 觀察肺組織病理改變及肺組織核因子κB( NF-κB) 表達(dá)情況。結(jié)果 LPS 組肺組織MPO、MDA 含量、W/D、肺損傷評分及肺組織NF-κB 表達(dá)均高于NS組( P 均 lt;0. 01) , 肺組織SOD 含量低于NS 組( P lt;0. 01) 。ED 組肺組織MPO、MDA 含量、W/D、肺損傷評分及肺組織NF-κB 表達(dá)均低于LPS組( P 均 lt;0. 01) , 仍高于NS 組( P 均 lt;0. 01) ;ED 組肺組織SOD 含量高于LPS 組( P lt;0. 01) , 仍低于NS 組( P lt;0. 01) 。結(jié)論 依達(dá)拉奉可以減輕LPS 誘導(dǎo)的大鼠ALI, 其機(jī)制可能與清除ROS, 降低了NF-кB 信號轉(zhuǎn)導(dǎo)通路的活化, 進(jìn)而減輕炎癥級聯(lián)放大效應(yīng)有關(guān)。
引用本文: 邢海波,李剛,徐亞平,高福云,劉國玲. 依達(dá)拉奉對膿毒癥大鼠肺損傷的保護(hù)作用. 中國呼吸與危重監(jiān)護(hù)雜志, 2009, 09(2): 176-180. doi: 復(fù)制
1. | 錢桂生. 在實踐中提高對急性肺損傷和急性呼吸窘迫綜合征新概念的認(rèn)識. 中國呼吸與危重監(jiān)護(hù)雜志, 2002, 1: 199-201. |
2. | Marshall JC. Inflammation, coagulopathy, and the pathogenesis of multiple organ dysfunction syndrome. Crit Care Med, 2001, 29: S99 -S106. |
3. | Amemiya S, Kamiya T, Nito C, et al. Anti-apoptotic and neuroprotective effects of edaravone following transient focal ischemia in rats. Eur J Pharmacol, 2005, 516: 125-130. |
4. | Eiichi O. Effect of a novel free radical scavenger, Edaravone ( MCI-186) , on acute brain infarction. Cerebrovasc Dis, 2003, 15: 222-229. |
5. | Fein AM, Calalang-Colucci MG. Acute lung injury and acute respiratory distress syndrome in sepsis and septic shock. Crit Care Clin, 2000, 16: 289-317. |
6. | Reyes YA, Shimoyama T, Akamatsu H, et al. MCI-186 ( edarvone) , a free radical scavenger, attenuates ischemia-reperfusion injury and activation of phospholipase A( 2) in an isolated rat lung model after 18h of cold preservation. Eur J Cardiothorac Surg, 2006 , 29: 304 -311. |
7. | :Acute lung injury and the acute respiratory distress syndrome:definitions and epidemiology. Thorax, 2002, 57: 452-458. |
8. | Mikawa K, Nishina K, Takao Y, et al. ONO-1714, a nitric oxide synthase-inhibitor, attenuates endotoxin-induced acute lung in rabbits. Anesth Analg, 2003, 97: 1751-1755. |
9. | 林孟相, 魏宏建, 付春來, 等. 膿毒癥患者血小板膜糖蛋白CD62p 和CD63 的表達(dá)及其臨床意義. 中國呼吸與危重監(jiān)護(hù)雜志, 2007, 6: 205-207, 229. |
10. | Sener G, Toklu H, Kapucu C, et al. Melatonin protects aganist oxidative organ injury in a rat model of sepsis. Surg Today, 2005 ,35: 52-59. |
11. | Atabai K, Matthay MA. The pulmonary physician in critical care. |
12. | Sessler CN, Bloomfield GL, Fowler AA 3rd. Current concepts of sepsis and acute lung injury. Clin Chest Med, 1996, 17: 213-235. |
13. | Cadenas S, Cadenas AM. Fighting the stranger-antioxidant protection against endotoxin toxicity. Toxicology, 2002, 180: 45-63. |
14. | Victor VM, Rocha M, De-la-Fuente M. Immune cells: free radicals and antioxidants in sepsis. Int Immunopharmacol, 2004 , 4: 327-347 . |
15. | Bhatia M, Moochhala S. Role of inflammatory mediators in the pathophysiology of acute respirstory distress syndrome. J Pathol,2004, 202: 145-156. |
16. | Di Filippo A, Ciapetti M, Prencipe D, et al. Experimentally-induced acute lung injury: the protective effect of hydroxyethyl starch. Ann Clin Lab Sci, 2006, 36: 345-352. |
17. | Nelson JL, Demichele SJ, Pacht ER, et al. Effect of enteral feeding with eicosapentaenoic acid, gamma-linolenic acid, and antioxidants on antioxidant status in patients with acute respiratory distress syndrome. JPEN J Parenter Enteral Nutr, 2003, 27: 98-104. |
18. | Chow CW, Herrera-Abreu MT, Suzuki T, et al. Oxidative stress and acute lung injury. Am J Respir Cell Mol Biol, 2003, 29: 427-431. |
19. | Victor VM, Rocha M, De-la-Fuente M. Immune cells: free radicals and antioxidants in sepsis. Int Immunopharmacol, 2004 , 4: 327-347 . |
20. | Altavilla D, Saitta A, Squadrito G, et al. Evidence for a role of nuclear factor-kappaB in acute hypovolemic hemorrhagic shock.Surgery, 2002, 131: 50-58. |
21. | Minhaz U, Tanaka M, Tsukamoto H, et al. Effect of MCI-186 on postischemic reperfusion injury in isolated rat heart. Free Radic Res, 1996, 24: 361-367. |
- 1. 錢桂生. 在實踐中提高對急性肺損傷和急性呼吸窘迫綜合征新概念的認(rèn)識. 中國呼吸與危重監(jiān)護(hù)雜志, 2002, 1: 199-201.
- 2. Marshall JC. Inflammation, coagulopathy, and the pathogenesis of multiple organ dysfunction syndrome. Crit Care Med, 2001, 29: S99 -S106.
- 3. Amemiya S, Kamiya T, Nito C, et al. Anti-apoptotic and neuroprotective effects of edaravone following transient focal ischemia in rats. Eur J Pharmacol, 2005, 516: 125-130.
- 4. Eiichi O. Effect of a novel free radical scavenger, Edaravone ( MCI-186) , on acute brain infarction. Cerebrovasc Dis, 2003, 15: 222-229.
- 5. Fein AM, Calalang-Colucci MG. Acute lung injury and acute respiratory distress syndrome in sepsis and septic shock. Crit Care Clin, 2000, 16: 289-317.
- 6. Reyes YA, Shimoyama T, Akamatsu H, et al. MCI-186 ( edarvone) , a free radical scavenger, attenuates ischemia-reperfusion injury and activation of phospholipase A( 2) in an isolated rat lung model after 18h of cold preservation. Eur J Cardiothorac Surg, 2006 , 29: 304 -311.
- 7. :Acute lung injury and the acute respiratory distress syndrome:definitions and epidemiology. Thorax, 2002, 57: 452-458.
- 8. Mikawa K, Nishina K, Takao Y, et al. ONO-1714, a nitric oxide synthase-inhibitor, attenuates endotoxin-induced acute lung in rabbits. Anesth Analg, 2003, 97: 1751-1755.
- 9. 林孟相, 魏宏建, 付春來, 等. 膿毒癥患者血小板膜糖蛋白CD62p 和CD63 的表達(dá)及其臨床意義. 中國呼吸與危重監(jiān)護(hù)雜志, 2007, 6: 205-207, 229.
- 10. Sener G, Toklu H, Kapucu C, et al. Melatonin protects aganist oxidative organ injury in a rat model of sepsis. Surg Today, 2005 ,35: 52-59.
- 11. Atabai K, Matthay MA. The pulmonary physician in critical care.
- 12. Sessler CN, Bloomfield GL, Fowler AA 3rd. Current concepts of sepsis and acute lung injury. Clin Chest Med, 1996, 17: 213-235.
- 13. Cadenas S, Cadenas AM. Fighting the stranger-antioxidant protection against endotoxin toxicity. Toxicology, 2002, 180: 45-63.
- 14. Victor VM, Rocha M, De-la-Fuente M. Immune cells: free radicals and antioxidants in sepsis. Int Immunopharmacol, 2004 , 4: 327-347 .
- 15. Bhatia M, Moochhala S. Role of inflammatory mediators in the pathophysiology of acute respirstory distress syndrome. J Pathol,2004, 202: 145-156.
- 16. Di Filippo A, Ciapetti M, Prencipe D, et al. Experimentally-induced acute lung injury: the protective effect of hydroxyethyl starch. Ann Clin Lab Sci, 2006, 36: 345-352.
- 17. Nelson JL, Demichele SJ, Pacht ER, et al. Effect of enteral feeding with eicosapentaenoic acid, gamma-linolenic acid, and antioxidants on antioxidant status in patients with acute respiratory distress syndrome. JPEN J Parenter Enteral Nutr, 2003, 27: 98-104.
- 18. Chow CW, Herrera-Abreu MT, Suzuki T, et al. Oxidative stress and acute lung injury. Am J Respir Cell Mol Biol, 2003, 29: 427-431.
- 19. Victor VM, Rocha M, De-la-Fuente M. Immune cells: free radicals and antioxidants in sepsis. Int Immunopharmacol, 2004 , 4: 327-347 .
- 20. Altavilla D, Saitta A, Squadrito G, et al. Evidence for a role of nuclear factor-kappaB in acute hypovolemic hemorrhagic shock.Surgery, 2002, 131: 50-58.
- 21. Minhaz U, Tanaka M, Tsukamoto H, et al. Effect of MCI-186 on postischemic reperfusion injury in isolated rat heart. Free Radic Res, 1996, 24: 361-367.