• 1 紹興市人民醫(yī)院ICU（浙江紹興 312000）;;
  • 2 衛(wèi)生部中日友好醫(yī)院ICU（北京 100029）;;
  • 3 衛(wèi)生部中日友好醫(yī)院臨床醫(yī)學(xué)研究所（北京 100029）;

目的  探討自由基清除劑依達(dá)拉奉( ED) 對膿毒癥致ALI 的保護(hù)作用。方法  24 只雄性Wistar 大鼠隨機(jī)分成3 組: 對照組( NS組) 、模型組( LPS 組) 及依達(dá)拉奉治療組( ED 組) 。LPS 組和ED 組采用尾靜脈注射LPS( 10 mg/ kg) 建立ALI 模型, ED 組隨后立即尾靜脈注射依達(dá)拉奉( 3 mg/kg) 。LPS 注射6 h后留取肺標(biāo)本, 分別測定肺組織濕/干重比值( W/D) 和肺組織勻漿中髓過氧化物酶( MPO) 、丙二醛( MDA) 和超氧化物歧化酶( SOD) 含量, 觀察肺組織病理改變及肺組織核因子κB( NF-κB) 表達(dá)情況。結(jié)果  LPS 組肺組織MPO、MDA 含量、W/D、肺損傷評分及肺組織NF-κB 表達(dá)均高于NS組( P 均 lt;0. 01) , 肺組織SOD 含量低于NS 組( P  lt;0. 01) 。ED 組肺組織MPO、MDA 含量、W/D、肺損傷評分及肺組織NF-κB 表達(dá)均低于LPS組( P 均 lt;0. 01) , 仍高于NS 組( P 均 lt;0. 01) ;ED 組肺組織SOD 含量高于LPS 組( P  lt;0. 01) , 仍低于NS 組( P  lt;0. 01) 。結(jié)論  依達(dá)拉奉可以減輕LPS 誘導(dǎo)的大鼠ALI, 其機(jī)制可能與清除ROS, 降低了NF-кB 信號轉(zhuǎn)導(dǎo)通路的活化, 進(jìn)而減輕炎癥級聯(lián)放大效應(yīng)有關(guān)。

引用本文： 邢海波,李剛,徐亞平,高福云,劉國玲. 依達(dá)拉奉對膿毒癥大鼠肺損傷的保護(hù)作用. 中國呼吸與危重監(jiān)護(hù)雜志, 2009, 09(2): 176-180. doi: 復(fù)制

1. 錢桂生. 在實踐中提高對急性肺損傷和急性呼吸窘迫綜合征新概念的認(rèn)識. 中國呼吸與危重監(jiān)護(hù)雜志, 2002, 1: 199-201.
2. Marshall JC. Inflammation, coagulopathy, and the pathogenesis of multiple organ dysfunction syndrome. Crit Care Med, 2001, 29: S99 -S106.
3. Amemiya S, Kamiya T, Nito C, et al. Anti-apoptotic and neuroprotective effects of edaravone following transient focal ischemia in rats. Eur J Pharmacol, 2005, 516: 125-130.
4. Eiichi O. Effect of a novel free radical scavenger, Edaravone ( MCI-186) , on acute brain infarction. Cerebrovasc Dis, 2003, 15: 222-229.
5. Fein AM, Calalang-Colucci MG. Acute lung injury and acute respiratory distress syndrome in sepsis and septic shock. Crit Care Clin, 2000, 16: 289-317.
6. Reyes YA, Shimoyama T, Akamatsu H, et al. MCI-186 ( edarvone) , a free radical scavenger, attenuates ischemia-reperfusion injury and activation of phospholipase A( 2) in an isolated rat lung model after 18h of cold preservation. Eur J Cardiothorac Surg, 2006 , 29: 304 -311.
7. :Acute lung injury and the acute respiratory distress syndrome:definitions and epidemiology. Thorax, 2002, 57: 452-458.
8. Mikawa K, Nishina K, Takao Y, et al. ONO-1714, a nitric oxide synthase-inhibitor, attenuates endotoxin-induced acute lung in rabbits. Anesth Analg, 2003, 97: 1751-1755.
9. 林孟相, 魏宏建, 付春來, 等. 膿毒癥患者血小板膜糖蛋白CD62p 和CD63 的表達(dá)及其臨床意義. 中國呼吸與危重監(jiān)護(hù)雜志, 2007, 6: 205-207, 229.
10. Sener G, Toklu H, Kapucu C, et al. Melatonin protects aganist oxidative organ injury in a rat model of sepsis. Surg Today, 2005 ,35: 52-59.
11. Atabai K, Matthay MA. The pulmonary physician in critical care.
12. Sessler CN, Bloomfield GL, Fowler AA 3rd. Current concepts of sepsis and acute lung injury. Clin Chest Med, 1996, 17: 213-235.
13. Cadenas S, Cadenas AM. Fighting the stranger-antioxidant protection against endotoxin toxicity. Toxicology, 2002, 180: 45-63.
14. Victor VM, Rocha M, De-la-Fuente M. Immune cells: free radicals and antioxidants in sepsis. Int Immunopharmacol, 2004 , 4: 327-347 .
15. Bhatia M, Moochhala S. Role of inflammatory mediators in the pathophysiology of acute respirstory distress syndrome. J Pathol,2004, 202: 145-156.
16. Di Filippo A, Ciapetti M, Prencipe D, et al. Experimentally-induced acute lung injury: the protective effect of hydroxyethyl starch. Ann Clin Lab Sci, 2006, 36: 345-352.
17. Nelson JL, Demichele SJ, Pacht ER, et al. Effect of enteral feeding with eicosapentaenoic acid, gamma-linolenic acid, and antioxidants on antioxidant status in patients with acute respiratory distress syndrome. JPEN J Parenter Enteral Nutr, 2003, 27: 98-104.
18. Chow CW, Herrera-Abreu MT, Suzuki T, et al. Oxidative stress and acute lung injury. Am J Respir Cell Mol Biol, 2003, 29: 427-431.
19. Victor VM, Rocha M, De-la-Fuente M. Immune cells: free radicals and antioxidants in sepsis. Int Immunopharmacol, 2004 , 4: 327-347 .
20. Altavilla D, Saitta A, Squadrito G, et al. Evidence for a role of nuclear factor-kappaB in acute hypovolemic hemorrhagic shock.Surgery, 2002, 131: 50-58.
21. Minhaz U, Tanaka M, Tsukamoto H, et al. Effect of MCI-186 on postischemic reperfusion injury in isolated rat heart. Free Radic Res, 1996, 24: 361-367.
  1. 1. 錢桂生. 在實踐中提高對急性肺損傷和急性呼吸窘迫綜合征新概念的認(rèn)識. 中國呼吸與危重監(jiān)護(hù)雜志, 2002, 1: 199-201.
  2. 2. Marshall JC. Inflammation, coagulopathy, and the pathogenesis of multiple organ dysfunction syndrome. Crit Care Med, 2001, 29: S99 -S106.
  3. 3. Amemiya S, Kamiya T, Nito C, et al. Anti-apoptotic and neuroprotective effects of edaravone following transient focal ischemia in rats. Eur J Pharmacol, 2005, 516: 125-130.
  4. 4. Eiichi O. Effect of a novel free radical scavenger, Edaravone ( MCI-186) , on acute brain infarction. Cerebrovasc Dis, 2003, 15: 222-229.
  5. 5. Fein AM, Calalang-Colucci MG. Acute lung injury and acute respiratory distress syndrome in sepsis and septic shock. Crit Care Clin, 2000, 16: 289-317.
  6. 6. Reyes YA, Shimoyama T, Akamatsu H, et al. MCI-186 ( edarvone) , a free radical scavenger, attenuates ischemia-reperfusion injury and activation of phospholipase A( 2) in an isolated rat lung model after 18h of cold preservation. Eur J Cardiothorac Surg, 2006 , 29: 304 -311.
  7. 7. :Acute lung injury and the acute respiratory distress syndrome:definitions and epidemiology. Thorax, 2002, 57: 452-458.
  8. 8. Mikawa K, Nishina K, Takao Y, et al. ONO-1714, a nitric oxide synthase-inhibitor, attenuates endotoxin-induced acute lung in rabbits. Anesth Analg, 2003, 97: 1751-1755.
  9. 9. 林孟相, 魏宏建, 付春來, 等. 膿毒癥患者血小板膜糖蛋白CD62p 和CD63 的表達(dá)及其臨床意義. 中國呼吸與危重監(jiān)護(hù)雜志, 2007, 6: 205-207, 229.
  10. 10. Sener G, Toklu H, Kapucu C, et al. Melatonin protects aganist oxidative organ injury in a rat model of sepsis. Surg Today, 2005 ,35: 52-59.
  11. 11. Atabai K, Matthay MA. The pulmonary physician in critical care.
  12. 12. Sessler CN, Bloomfield GL, Fowler AA 3rd. Current concepts of sepsis and acute lung injury. Clin Chest Med, 1996, 17: 213-235.
  13. 13. Cadenas S, Cadenas AM. Fighting the stranger-antioxidant protection against endotoxin toxicity. Toxicology, 2002, 180: 45-63.
  14. 14. Victor VM, Rocha M, De-la-Fuente M. Immune cells: free radicals and antioxidants in sepsis. Int Immunopharmacol, 2004 , 4: 327-347 .
  15. 15. Bhatia M, Moochhala S. Role of inflammatory mediators in the pathophysiology of acute respirstory distress syndrome. J Pathol,2004, 202: 145-156.
  16. 16. Di Filippo A, Ciapetti M, Prencipe D, et al. Experimentally-induced acute lung injury: the protective effect of hydroxyethyl starch. Ann Clin Lab Sci, 2006, 36: 345-352.
  17. 17. Nelson JL, Demichele SJ, Pacht ER, et al. Effect of enteral feeding with eicosapentaenoic acid, gamma-linolenic acid, and antioxidants on antioxidant status in patients with acute respiratory distress syndrome. JPEN J Parenter Enteral Nutr, 2003, 27: 98-104.
  18. 18. Chow CW, Herrera-Abreu MT, Suzuki T, et al. Oxidative stress and acute lung injury. Am J Respir Cell Mol Biol, 2003, 29: 427-431.
  19. 19. Victor VM, Rocha M, De-la-Fuente M. Immune cells: free radicals and antioxidants in sepsis. Int Immunopharmacol, 2004 , 4: 327-347 .
  20. 20. Altavilla D, Saitta A, Squadrito G, et al. Evidence for a role of nuclear factor-kappaB in acute hypovolemic hemorrhagic shock.Surgery, 2002, 131: 50-58.
  21. 21. Minhaz U, Tanaka M, Tsukamoto H, et al. Effect of MCI-186 on postischemic reperfusion injury in isolated rat heart. Free Radic Res, 1996, 24: 361-367.

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