• 中山大學(xué)附屬第一醫(yī)院呼吸內(nèi)科（廣東廣州 510080）;

目的 探討水通道蛋白1( AQP1 ) 在胸腔積液發(fā)生機制中的作用。方法 SD 大鼠按實驗設(shè)計分為5 組( n =24) : 地塞米松組、內(nèi)毒素組、紅霉素組、高滲鹽水組及對照組, 通過免疫組織化學(xué)染色測定AQP1 在胸膜上的表達和定位; 采用Real-time RT-PCR 方法對不同刺激因子作用下不同時間胸膜組織AQP1 的基因表達進行定量分析。結(jié)果 大鼠臟、壁層胸膜間皮細胞及壁層胸膜下毛細血管內(nèi)皮上均存在AQP1 的表達, 臟層胸膜間皮細胞上AQP1 的表達較壁層胸膜明顯[ ( 7. 43 ±2. 02) ×104 copy / μg 比( 4. 14 ±1. 12) ×104 copy/ μg, P  lt;0. 05] 。地塞米松、高滲鹽水上調(diào)大鼠胸膜組織AQP1 的表達( P  lt;0. 05) ; 紅霉素、內(nèi)毒素下調(diào)胸膜組織上AQP1 的表達( P  lt;0. 05) 。結(jié)論 地塞米松、內(nèi)毒素、紅霉素和高滲鹽水刺激下胸膜組織AQP1 表達的改變, 提示存在炎癥因子和滲透梯度下調(diào)控AQP1 的途徑, 胸膜組織上AQP1 可能參與了炎癥性胸腔積液的分泌和吸收。

引用本文： 廖槐,謝燦茂. 水通道蛋白1 與胸腔積液發(fā)生機制的體內(nèi)研究. 中國呼吸與危重監(jiān)護雜志, 2009, 09(2): 151-155. doi: 復(fù)制

1. Preston GM, Agre P. Isolation of the cDNA for erythrocytes integral membrane protein of 28 kD:Member of an ancient channel family.Proc Natl Acad Sci USA, 1991, 88: 11110-11114.
2. Verkman AS. Physiological importance of aquaporin water channels.Ann Med, 2002, 34: 192-200.
3. Agre P. The aquaporin water channels. Proc Am Soc, 2006, 3: 5-13.
4. King LS, Nielsen S, Agre P. Respiratory Aquaporins in Lung Inflammation. Am J Respir Cell Mol Biol, 2000 , 22: 8 -10 .
5. Towne EE, Harrod KS. Decreased Expression of Aquaporin( AQP) 1 and AQP5 in Mouse Lung after Acute Viral Infection. Am J Respir Cell Mol Biol, 2000, 22: 34-44.
6. 張偉, 謝燦茂, 黃建強, 等. 高滲液對大鼠胸膜間皮細胞水通道蛋白1 表達的影響. 中華醫(yī)學(xué)雜志, 2007, 87: 2508-2511.
7. 張偉, 謝燦茂. 角叉菜膠致炎癥性胸腔積液大鼠胸膜上水通道蛋白1 的表達. 中國呼吸與危重監(jiān)護雜志, 2008, 7: 190-194.
8. Yang B, Folkesson HG, Yang J, et al. Reduced osmotic water permeability of the peritoneal barrier in aquaporin-1 knockout mice.Am J Physiol Cell Physiol, 1999, 276: c76-c81.
9. Moon C, King LS, Agre P. AQP1 expression in erythroleukemia cells: genetic regulation of glucocorticoid and chemical induction.Am J Physiol Cell Physiol, 1997, 273: c1562-c1570.
10. Jiao G, Li E, Yu R. Decreased expression of AQP1 and AQP5 in acute injured lungs in rats. Chin Med J, 2002, 115: 963-967.
11. Song Y, Fukuda N, Bai C, et al. Role of aquaporins in alveolar fluid clearance in neonatal and adult lung, and in oedema formation following acute lung injury: studies in transgenic aquaporin null mice. J Physiol, 2000, 525: 771-779.
12. 黃建強, 謝燦茂. 紅霉素對人胸膜間皮細胞生長及腫瘤壞死因子-α、轉(zhuǎn)化生長因子-β1 分泌和縫隙連接蛋白43 表達的影響.中華結(jié)核和呼吸雜志, 2003, 26: 206-209.
13. Miserocchi G. Physiology and pathophysiology of pleural fluid turnover. Eur Respir J, 1997, 10: 219-225.
14. Miserocchi G, Venturoli D, Negrini D, et al.Model of pleural fluid turnover. J Appl Physiol, 1993, 75: 1798-1806.
15. Wiener-Kronish JP, Albertine KH, Lick V, et al. Protein egress and entry rates in pleural fluid and plasma in sheep. J Appl Physiol, 1984, 56: 459-463.
16. Lee CH, Wang WJ, Lan RS, et al. Corticosteroids in the treatment of tuberculous pleurisy. A double-blind, placebo-controlled,randomized study. Chest, 1988, 94: 256-1259.
17. Xie CM, Teixeira LR, McGovern JP, et al. Systemic corticosteroids decrease the effectiveness of talc pleurodesis. AmJ Respir Crit Care Med, 1998, 157: 1441-1444.
18. Gopi A, Madhavan SM, Sharma SK, et al. Diagnosis and treatment of tuberculous pleural effusion in 2006. Chest, 2007, 131: 880-889.
19. Sartori C, Matthay MA. Alveolar epithelial fluid transport in acute lung injury: new insights. Eur Respir J, 2002, 20: 1299-1313.
20. Leitch V, Agre P, King LS. Altered ubiquitination and stability of aquaporin-1 in hypertonic stress. PNAS, 2001, 98: 2894-2898.
21. Hoffert JD, Agre P, King LS. Hypertonic Induction of Aquaporin-5 Expression through an ERK-dependent Pathway. J Biol Chem,2000, 275: 9070-9077.
22. Song Y, Matthay MA, Verkman AS. Role of Aquaporin water channels in Pleural fluid dynamics. Am J Physiol Cell Physiol,2000, 279: 1744-1750.
23. Fang W, Qian J, Yu Z, et al. Expression of aquaporin-1 in the human peritoneum and the effect of peritoneal dialysis on its expression. Chin Med J, 2003, 116: 1370-1373.
24. 謝艷萍, 陳才平, 王建春, 等. 脂多糖、腫瘤壞死因子-α、白細胞介素-1β鼠肺微血管內(nèi)皮細胞水通道蛋白-1 表達的影響. 中國呼吸與危重監(jiān)護雜志, 2005, 4: 142-145.
  1. 1. Preston GM, Agre P. Isolation of the cDNA for erythrocytes integral membrane protein of 28 kD:Member of an ancient channel family.Proc Natl Acad Sci USA, 1991, 88: 11110-11114.
  2. 2. Verkman AS. Physiological importance of aquaporin water channels.Ann Med, 2002, 34: 192-200.
  3. 3. Agre P. The aquaporin water channels. Proc Am Soc, 2006, 3: 5-13.
  4. 4. King LS, Nielsen S, Agre P. Respiratory Aquaporins in Lung Inflammation. Am J Respir Cell Mol Biol, 2000 , 22: 8 -10 .
  5. 5. Towne EE, Harrod KS. Decreased Expression of Aquaporin( AQP) 1 and AQP5 in Mouse Lung after Acute Viral Infection. Am J Respir Cell Mol Biol, 2000, 22: 34-44.
  6. 6. 張偉, 謝燦茂, 黃建強, 等. 高滲液對大鼠胸膜間皮細胞水通道蛋白1 表達的影響. 中華醫(yī)學(xué)雜志, 2007, 87: 2508-2511.
  7. 7. 張偉, 謝燦茂. 角叉菜膠致炎癥性胸腔積液大鼠胸膜上水通道蛋白1 的表達. 中國呼吸與危重監(jiān)護雜志, 2008, 7: 190-194.
  8. 8. Yang B, Folkesson HG, Yang J, et al. Reduced osmotic water permeability of the peritoneal barrier in aquaporin-1 knockout mice.Am J Physiol Cell Physiol, 1999, 276: c76-c81.
  9. 9. Moon C, King LS, Agre P. AQP1 expression in erythroleukemia cells: genetic regulation of glucocorticoid and chemical induction.Am J Physiol Cell Physiol, 1997, 273: c1562-c1570.
  10. 10. Jiao G, Li E, Yu R. Decreased expression of AQP1 and AQP5 in acute injured lungs in rats. Chin Med J, 2002, 115: 963-967.
  11. 11. Song Y, Fukuda N, Bai C, et al. Role of aquaporins in alveolar fluid clearance in neonatal and adult lung, and in oedema formation following acute lung injury: studies in transgenic aquaporin null mice. J Physiol, 2000, 525: 771-779.
  12. 12. 黃建強, 謝燦茂. 紅霉素對人胸膜間皮細胞生長及腫瘤壞死因子-α、轉(zhuǎn)化生長因子-β1 分泌和縫隙連接蛋白43 表達的影響.中華結(jié)核和呼吸雜志, 2003, 26: 206-209.
  13. 13. Miserocchi G. Physiology and pathophysiology of pleural fluid turnover. Eur Respir J, 1997, 10: 219-225.
  14. 14. Miserocchi G, Venturoli D, Negrini D, et al.Model of pleural fluid turnover. J Appl Physiol, 1993, 75: 1798-1806.
  15. 15. Wiener-Kronish JP, Albertine KH, Lick V, et al. Protein egress and entry rates in pleural fluid and plasma in sheep. J Appl Physiol, 1984, 56: 459-463.
  16. 16. Lee CH, Wang WJ, Lan RS, et al. Corticosteroids in the treatment of tuberculous pleurisy. A double-blind, placebo-controlled,randomized study. Chest, 1988, 94: 256-1259.
  17. 17. Xie CM, Teixeira LR, McGovern JP, et al. Systemic corticosteroids decrease the effectiveness of talc pleurodesis. AmJ Respir Crit Care Med, 1998, 157: 1441-1444.
  18. 18. Gopi A, Madhavan SM, Sharma SK, et al. Diagnosis and treatment of tuberculous pleural effusion in 2006. Chest, 2007, 131: 880-889.
  19. 19. Sartori C, Matthay MA. Alveolar epithelial fluid transport in acute lung injury: new insights. Eur Respir J, 2002, 20: 1299-1313.
  20. 20. Leitch V, Agre P, King LS. Altered ubiquitination and stability of aquaporin-1 in hypertonic stress. PNAS, 2001, 98: 2894-2898.
  21. 21. Hoffert JD, Agre P, King LS. Hypertonic Induction of Aquaporin-5 Expression through an ERK-dependent Pathway. J Biol Chem,2000, 275: 9070-9077.
  22. 22. Song Y, Matthay MA, Verkman AS. Role of Aquaporin water channels in Pleural fluid dynamics. Am J Physiol Cell Physiol,2000, 279: 1744-1750.
  23. 23. Fang W, Qian J, Yu Z, et al. Expression of aquaporin-1 in the human peritoneum and the effect of peritoneal dialysis on its expression. Chin Med J, 2003, 116: 1370-1373.
  24. 24. 謝艷萍, 陳才平, 王建春, 等. 脂多糖、腫瘤壞死因子-α、白細胞介素-1β鼠肺微血管內(nèi)皮細胞水通道蛋白-1 表達的影響. 中國呼吸與危重監(jiān)護雜志, 2005, 4: 142-145.

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