目的 探討不可分型流感嗜血桿菌（NTHi）作用于呼吸道上皮細胞株（A549），表達前炎癥細胞因子的變化及其干預措施。方法 用NTHi、NTHi+紅霉素（10 mg/L）、NTHi+慶大霉素（100 mg/L）、NTHi+地塞米松（100 μmol/L）分別感染A549細胞，用核因子κB（NF-κB）抑制劑二硫氨基甲酸吡啶（PDTC，200 μmol/L）預處理NTHi感染的A549細胞，24 h后觀察細胞的形態(tài)學改變，并檢測上清液中白細胞介素8（IL-8）、腫瘤壞死因子α（TNF-α）的水平和細胞間黏附分子1（ICAM-1）的表達。結(jié)果 NTHi作用A549細胞24 h后，引起部分上皮細胞變形、死亡。NTHi能顯著誘導A549細胞株分泌IL-8、表達ICAM-1，紅霉素能夠抑制其誘導作用；慶大霉素具有殺滅NTHi作用，但不能抑制IL-8分泌和ICAM-1表達；地塞米松能抑制A549分泌IL-8，降低ICAM-1的表達，但不能殺滅NTHi。PDTC（200 μmol/L）能阻斷A549細胞在NTHi作用后IL-8的高表達。TNF-α在各組培養(yǎng)上清中均不能檢測到。結(jié)論 NTHi顯著誘導人肺上皮細胞系表達前炎癥因子，該作用可能通過NF-κB通路。紅霉素在殺滅NTHi的同時還具有抗炎作用。
引用本文： 修清玉,錢建美,王桂芳,黃海,方正. 不可分型流感嗜血桿菌誘導A549細胞分泌和表達前炎癥細胞因子及機制研究. 中國呼吸與危重監(jiān)護雜志, 2008, 08(3): 210-213. doi: 復制
| 1. | 1 方正，修清玉，羅文侗. 慢性阻塞性肺疾病中的流感嗜血桿菌感染. 第二軍醫(yī)大學學報 1999, 20:624-626. |
| 2. | 4 錢建美，修清玉，王桂芳,等.不可分型流感嗜血桿菌對氣管黏膜上皮細胞的作用.第二軍醫(yī)大學學報,2004，25：769-772. |
| 3. | Sethi S, Evans N, Grant BJ, et al. New strains of bacteria and exacerbations of chronic obstructive pulmonary disease. N Engl J Med, 2002, 347:465-471. |
| 4. | Murphy TF, Brauer AL, Schiffmacher AT,et al.Persistent colonization by Haemophilus influenzae in chronic obstructive pulmonary disease. Am J Respir Crit Care Med, 2004,170:266–272. |
| 5. | Khair OA, Devalia JL, Abdelaziz MM, et al. Effect of Haemophilus influenzae endotoxin on the synthesis of IL-6,IL-8,TNF-α and expression of ICAM-1 in cultured human bronchial epithelial cells. Eur Respir J,1994,7:2109-2116. |
| 6. | Marcet B, Libert F, Boeynaems JM, et al. Extracellular nucleotides induce COX-2 up-regulation and prostaglandin E (2) production in human A549 alveolar type II epithelial cells. Eur J Pharmacol, 2007, 566: 167-171. |
| 7. | Carterson AJ, Höner zu Bentrup K, Ott CM, et al .A549 lung epithelial cells grown as three-dimensional aggregates: alternative tissue culture model for Pseudomonas aeruginosa pathogenesis. Infect. Immun, 2005, 73:1129-1140. |
| 8. | Clemans DL, Bauer RJ, Hanson JA, et al. Induction of proinflammatory cytokines from human respiratory epithelial cells after stimulation by nontypeable Haemophilus influenzae. Infect Immun, 2000, 68:4430-4440. |
| 9. | Frick AG, Joseph TD, Pang LY, et al. Haemophilus influenzae stimulates ICAM-1 expression on respiratory epithelial cells. J Immunol, 2000, 164:4185-4196. |
- 1. 1 方正，修清玉，羅文侗. 慢性阻塞性肺疾病中的流感嗜血桿菌感染. 第二軍醫(yī)大學學報 1999, 20:624-626.
- 2. 4 錢建美，修清玉，王桂芳,等.不可分型流感嗜血桿菌對氣管黏膜上皮細胞的作用.第二軍醫(yī)大學學報,2004，25：769-772.
- 3. Sethi S, Evans N, Grant BJ, et al. New strains of bacteria and exacerbations of chronic obstructive pulmonary disease. N Engl J Med, 2002, 347:465-471.
- 4. Murphy TF, Brauer AL, Schiffmacher AT,et al.Persistent colonization by Haemophilus influenzae in chronic obstructive pulmonary disease. Am J Respir Crit Care Med, 2004,170:266–272.
- 5. Khair OA, Devalia JL, Abdelaziz MM, et al. Effect of Haemophilus influenzae endotoxin on the synthesis of IL-6,IL-8,TNF-α and expression of ICAM-1 in cultured human bronchial epithelial cells. Eur Respir J,1994,7:2109-2116.
- 6. Marcet B, Libert F, Boeynaems JM, et al. Extracellular nucleotides induce COX-2 up-regulation and prostaglandin E (2) production in human A549 alveolar type II epithelial cells. Eur J Pharmacol, 2007, 566: 167-171.
- 7. Carterson AJ, Höner zu Bentrup K, Ott CM, et al .A549 lung epithelial cells grown as three-dimensional aggregates: alternative tissue culture model for Pseudomonas aeruginosa pathogenesis. Infect. Immun, 2005, 73:1129-1140.
- 8. Clemans DL, Bauer RJ, Hanson JA, et al. Induction of proinflammatory cytokines from human respiratory epithelial cells after stimulation by nontypeable Haemophilus influenzae. Infect Immun, 2000, 68:4430-4440.
- 9. Frick AG, Joseph TD, Pang LY, et al. Haemophilus influenzae stimulates ICAM-1 expression on respiratory epithelial cells. J Immunol, 2000, 164:4185-4196.