目的 通過建立大鼠肺癌模型，研究山葵提取液對肺癌早期分子標志物hnRNP A2/B1的調(diào)節(jié)作用，進一步闡明山葵抗肺癌作用的分子機制。方法 將36只Wistar大鼠隨機分為肺癌模型組（對照組）和山葵治療組（實驗組）。按1 mL碘油內(nèi)含3-甲基膽蒽（MCA）50 mg配制致癌質(zhì)碘油。每只大鼠均用MCA碘油溶液0.1 mL在左肺葉支氣管內(nèi)灌注誘癌。實驗組于誘癌前1周開始胃飼山葵提取液至動物被處死，對照組于同一時間胃飼等量生理鹽水。造模后第30、60及90 d分批處死大鼠，每次6只。免疫組織化學法和RT-PCR法檢測肺癌組織hnRNP A2/B1的蛋白和基因表達。結(jié)果 山葵提取液能降低肺癌組織hnRNP A2/B1的蛋白表達，在第30，60及90 d的抑制率分別為51.0%，51.0%和45.1%。山葵提取液同時降低了hnRNP A2/B1 mRNA的表達，在第60和90 d的抑制率分別為79.5%和58.0%。結(jié)論 山葵提取液能降低肺癌早期分子標志物hnRNP A2/B1的表達，可能是其抗肺癌的分子機制之一。
引用本文： 陶然,李為民,陳文彬. 山葵對肺癌組織hnRNP A2/B1表達的影響. 中國呼吸與危重監(jiān)護雜志, 2008, 08(2): 107-109. doi: 復制
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- 1. 楊艷,曹箭.肺癌早期診斷相關(guān)標記物的研究進展.診斷病理學雜志,2007,14:229-231.
- 2. Genoveva Murillo,Rajjendra G Mehta.Cruciferous Vegetables and Cancer Prevention.Nutrition Cancer,2001,41:17-28.
- 3. Cohen JH,Kristal AR,Stanford JL.Fruit and vegetable intakes and prostate cancer risk.J Natl Cancer Inst,2000,92:61-68.
- 4. Watanabe M,Ohata M,Hayakawa S,et al.Identification of 6-methylsulfinylhexyl isothiocyanate as an apoptosis-inducing component in wasabi.Phytochemistry,2003,62:733-739.
- 5. Morimitsu Y,Nakagawa Y,Hayashi K,et al.A sulforaphane analogue that potently activates the Nrf2-dependent detoxification pathway.J Biol Chem,2002,277:3456-3463.
- 6. 田鴻生,劉銘球,高文琴,等.建立動物肺癌模型的方法學研究——Ⅱ.肺葉支氣管內(nèi)灌注致癌質(zhì)碘油溶液誘發(fā)大白鼠肺癌的新方法.腫瘤防治研究,1982,9:2-4.
- 7. 孫敬方.動物實驗方法學.北京:人民衛(wèi)生出版社,2004,308-309.
- 8. 黃明深.山葵調(diào)味品原料中異硫氰酸酯的含量及影響因素研究.中國調(diào)味品,2005,6:21-23.
- 9. Yano T,Yajima S,Virgona N,et al.The effect of 6-methylthiohexyl isothiocyanate isolated from Wasabia japonica (wasabi) on 4-(methylnitrosamino)-1-(3-pyridyl)-1-buatnone-induced lung tumorigenesis in mice.Cancer Lett,2000,155:115-120.
- 10. Tockman MS,Mulshine JL,Piantadosi S,et al.Prospective detection of preclinical lung cancer:results from two studies of heterogeneous nuclear ribonucleoprotein A2/B1 overexpression.Clin Cancer Res,1997,3:2237-2246.
- 11. Fujimoto N,Sueoka N,Sueoka E,et al.Lung cancer prevention with(-)-epigallocatechin gallate using monitoring by heterogeneous nuclear ribonucleoprotein B1.Int J Oncol,2002,20:1233-1239.
- 12. Garayoa M,Man YG,Martinez A,et al.Downregulation of hnRNP A2/B1 expression in tumor cells under prolonged hypoxia.Am J Respir Cell Mol Biol,2003,28:80-85.