• 1 重慶第三軍醫(yī)大學(xué)新橋醫(yī)院全軍呼吸疾病研究所（重慶 400037）;;
  • 2 全軍腫瘤中心;

目的 研究FasL基因轉(zhuǎn)染樹突狀細胞（DC）對屋塵螨致敏/激發(fā)小鼠氣道炎癥的影響。方法 將FasL基因轉(zhuǎn)染的DC和對照DC（未轉(zhuǎn)染DC）分別經(jīng)氣管注入屋塵螨致敏/激發(fā)小鼠體內(nèi)，病理切片觀察小鼠肺部炎癥，取支氣管肺泡灌洗液（BALF），行BALF細胞總數(shù)及分類計數(shù)，ELISA檢測BALF的白細胞介素-4（IL-4）、IL-5和g干擾素（IFN-g）水平。結(jié)果 FasL基因轉(zhuǎn)染的DC過繼處理屋塵螨致敏/激發(fā)小鼠，能顯著地減輕致敏/激發(fā)小鼠氣道變應(yīng)性炎癥，減少BALF中細胞總數(shù)和嗜酸粒細胞比例，降低BALF中IL-4和IL-5的水平，增高IFN-?水平。結(jié)論 FasL基因轉(zhuǎn)染的DC過繼處理屋塵螨致敏/激發(fā)小鼠能抑制Th2細胞活化，抑制變應(yīng)性氣道炎癥。

引用本文： 王彥,卓文磊,畢玉田,吳奎,王長征,宋云熙,夏俊波. FasL基因轉(zhuǎn)染樹突狀細胞對屋塵螨致敏/激發(fā)小鼠氣道炎癥影響的研究. 中國呼吸與危重監(jiān)護雜志, 2007, 6(1): 15-18. doi: 復(fù)制

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2. Bohana-Kashtan O，Civin CI.Fas ligand as a tool for immunosuppression and generation of immune tolerance.Stem Cells，2004，22：908-924.
3. Griffith TS，Brunner T，F(xiàn)letcher SM，et al.Fas ligand-induced apoptosis as a mechanism of immune privilege.Science，1995，270：1189-1192.
4. Askenasy N，Yolcu ES，Yaniv I，et al.Induction of tolerance using Fas ligand：a double-edged immunomodulator.Blood，2005，105：1396-1404.
5. Quah BJ，O’Neill HC.Maturation of function in dendritic cells for tolerance and immunity.J Cell Mol Med，2005，9：643-654.
6. Zhang HG，Mountz JD，F(xiàn)leck M，et al.Specific deletion of autoreactive T cells by adenovirus-transfected，F(xiàn)as ligand-producing antigen-presenting cells.Immunol Res，2002，26：235-246.
7. 夏俊波，吳奎，孫鯤，等.小鼠骨髓來源的樹突狀細胞體外擴增與鑒定.第三軍醫(yī)大學(xué)學(xué)報，2005，27：942-944.
8. Hansen G，Berry G，DeKruyff RH，et al.Allergen-specific Th1 cells fail to counterbalance Th2 cell-induced airway hyperreactivity but cause severe airway inflammation.J Clin Invest，1999，103：175-183.
9. Dahl ME，Dabbagh K，Liggitt D，et al.Viral-induced T helper type 1 responses enhance allergic disease by effects on lung dendritic cells.Nat Immunol，2004，5：337-343.
10. Chuang YH，F(xiàn)u CL，Lo YC，et al.Adenovirus expressing Fas ligand gene decreases airway hyper-responsiveness and eosinophilia in a murine model of asthma.Gene Ther，2004，11：1497-1505.
11. Min WP，Gorczynski R，Huang XY，et al.Dendritic cells genetically engineered to express Fas ligand induce donor-specific hyporesponsiveness and prolong allograft survival.J Immunol，2000；164：161-167.
12. Zhang HG，Su X，Liu D，et al.Induction of specific T cell tolerance by Fas ligand-expressing antigen presenting cells.J Immunol，1999，162：1423-1430.
13. Zhan HG，Mountz JD，F(xiàn)leck M，et al.Specific deletion of autoreactive T cells by adenovirus-transfected，F(xiàn)as ligand-producing antigen-presenting cells.Immunol Res，2002，26：235-246.
14. Zhang HG，Liu D，Heike Y，et al.Induction of specific T-cell tolerance by adenovirus-transfected，F(xiàn)as ligand-producing antigen presenting cells.Nat Biotechnol，1998，16：1045-1049.
15. Matsue H，Matsue K，Walters M，et al.Induction of antigen-specific immunosuppression by CD95L cDNA-transfected ’killer’ dendritic cells.Nat Med，1999，5：930-937.
16. Kusuhara M，Matsue K，Edelbaum D，et al.Killing of naïve T cells by CD95L-transfected dendritic cells(DC)：in vivo study using killer DC-DC hybrids and CD4(+) T cells from DO11.10 mice.Eur J Immunol，2002，32：1035-1043.
  1. 1. Suda T，Takahashi T，Golstein P，et al.Molecular cloning and expression of the Fas ligand，a novel member of the tumor necrosis factor family.Cell，1993，75：1169-1178.
  2. 2. Bohana-Kashtan O，Civin CI.Fas ligand as a tool for immunosuppression and generation of immune tolerance.Stem Cells，2004，22：908-924.
  3. 3. Griffith TS，Brunner T，F(xiàn)letcher SM，et al.Fas ligand-induced apoptosis as a mechanism of immune privilege.Science，1995，270：1189-1192.
  4. 4. Askenasy N，Yolcu ES，Yaniv I，et al.Induction of tolerance using Fas ligand：a double-edged immunomodulator.Blood，2005，105：1396-1404.
  5. 5. Quah BJ，O’Neill HC.Maturation of function in dendritic cells for tolerance and immunity.J Cell Mol Med，2005，9：643-654.
  6. 6. Zhang HG，Mountz JD，F(xiàn)leck M，et al.Specific deletion of autoreactive T cells by adenovirus-transfected，F(xiàn)as ligand-producing antigen-presenting cells.Immunol Res，2002，26：235-246.
  7. 7. 夏俊波，吳奎，孫鯤，等.小鼠骨髓來源的樹突狀細胞體外擴增與鑒定.第三軍醫(yī)大學(xué)學(xué)報，2005，27：942-944.
  8. 8. Hansen G，Berry G，DeKruyff RH，et al.Allergen-specific Th1 cells fail to counterbalance Th2 cell-induced airway hyperreactivity but cause severe airway inflammation.J Clin Invest，1999，103：175-183.
  9. 9. Dahl ME，Dabbagh K，Liggitt D，et al.Viral-induced T helper type 1 responses enhance allergic disease by effects on lung dendritic cells.Nat Immunol，2004，5：337-343.
  10. 10. Chuang YH，F(xiàn)u CL，Lo YC，et al.Adenovirus expressing Fas ligand gene decreases airway hyper-responsiveness and eosinophilia in a murine model of asthma.Gene Ther，2004，11：1497-1505.
  11. 11. Min WP，Gorczynski R，Huang XY，et al.Dendritic cells genetically engineered to express Fas ligand induce donor-specific hyporesponsiveness and prolong allograft survival.J Immunol，2000；164：161-167.
  12. 12. Zhang HG，Su X，Liu D，et al.Induction of specific T cell tolerance by Fas ligand-expressing antigen presenting cells.J Immunol，1999，162：1423-1430.
  13. 13. Zhan HG，Mountz JD，F(xiàn)leck M，et al.Specific deletion of autoreactive T cells by adenovirus-transfected，F(xiàn)as ligand-producing antigen-presenting cells.Immunol Res，2002，26：235-246.
  14. 14. Zhang HG，Liu D，Heike Y，et al.Induction of specific T-cell tolerance by adenovirus-transfected，F(xiàn)as ligand-producing antigen presenting cells.Nat Biotechnol，1998，16：1045-1049.
  15. 15. Matsue H，Matsue K，Walters M，et al.Induction of antigen-specific immunosuppression by CD95L cDNA-transfected ’killer’ dendritic cells.Nat Med，1999，5：930-937.
  16. 16. Kusuhara M，Matsue K，Edelbaum D，et al.Killing of naïve T cells by CD95L-transfected dendritic cells(DC)：in vivo study using killer DC-DC hybrids and CD4(+) T cells from DO11.10 mice.Eur J Immunol，2002，32：1035-1043.