為觀察促肝細(xì)胞生長素(pHGF)對(duì)肝硬變大鼠部分肝切除后肝再生的影響，應(yīng)用IBASⅡ全自動(dòng)圖像分析儀檢測肝硬變大鼠術(shù)后肝細(xì)胞DNA倍體率及肝臟組化染色后各組酶灰度值(OPTDM)的變化，并通過檢測血清谷丙轉(zhuǎn)氨酶(SGPT)及吲哚青綠15分鐘滯留率(ICG15)以了解殘肝功能。結(jié)果：實(shí)驗(yàn)組(A組)3～4倍體肝細(xì)胞明顯減少，2倍體及多倍體肝細(xì)胞明顯增多；術(shù)后第1天各組酶灰度值無顯著差異，第2、5天琥珀酸脫氫酶(SDH)和乳酸脫氫酶(LDH)明顯增高，而堿性磷酸酶(AkP)和酸性磷酸酶(AcP)則明顯減少；A組術(shù)后第5天SGPT和ICG１５明顯降低。本實(shí)驗(yàn)結(jié)果提示：pHGF不僅能促使肝硬變大鼠肝切除后殘肝的再生，而且能夠促進(jìn)再生肝細(xì)胞功能成熟及殘肝功能的恢復(fù)。
引用本文： 張敬杰,艾中立,賈貴生,劉志蘇. 促肝細(xì)胞生長素對(duì)肝硬變大鼠部分肝切除后肝再生影響的實(shí)驗(yàn)研究. 中國普外基礎(chǔ)與臨床雜志, 1998, 5(4): 199-201. doi: 復(fù)制
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- 4. Michalopoulous GK, Zarnegar R. Hepatocyte growth factor. Hepatology, 1992; 15(1)∶149.
- 5. Kinoshita T, Hiraos, Matsumotok, et al. Possible endocrine control by hepatocyte growth factor of liver regeneration after partial hepatectomy. Biochem Biophys Res Commun, 1991; 177(1)∶330.
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