為觀察灌注壓力對肝移植物活性的影響，實驗選擇SD大鼠，經(jīng)門靜脈在不同壓力下灌注4℃的Collin’s液以保存肝臟，然后應(yīng)用高效液相色譜儀檢測各組的高能磷酸化合物，制備出肝細(xì)胞懸液后采用陽離子測定儀測定肝細(xì)胞內(nèi)游離鈣濃度，并同時觀察了肝臟的超微結(jié)構(gòu)變化及光鏡病理改變。結(jié)果發(fā)現(xiàn)：隨灌注壓力的升高，肝臟的能量代謝、肝細(xì)胞內(nèi)游離鈣濃度以及超微結(jié)構(gòu)均有明顯改變。本實驗結(jié)果提示：不適當(dāng)?shù)墓嘧毫蓪?dǎo)致肝臟移植物的能量代謝的變化及肝細(xì)胞的鈣超載，從而影響肝移植物的活力； 為了取得高質(zhì)量的供肝，應(yīng)采用低溫低壓灌注。
引用本文： 張浩,王學(xué)浩,張峰. 灌注壓力對肝移植物活性的影響. 中國普外基礎(chǔ)與臨床雜志, 1999, 6(3): 140-142. doi: 復(fù)制
版權(quán)信息： ?四川大學(xué)華西醫(yī)院華西期刊社《中國普外基礎(chǔ)與臨床雜志》版權(quán)所有，未經(jīng)授權(quán)不得轉(zhuǎn)載、改編
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