目的 觀察血管內(nèi)皮生長因子（VEGF）及β3整合素在肉芽組織血管生成中表達的動態(tài)變化。方法 RT-PCR法及免疫組織化學(xué)法觀察人正常皮下組織、增殖期肉芽組織（傷后7~12天）和成熟期肉芽組織（傷后30~35天）VEGF及β3整合素mRNA及蛋白的表達。 結(jié)果 VEGF及β3整合素在正常皮下組織表達很低，在增殖期肉芽組織中表達明顯升高，而肉芽組織成熟后其表達又降低。結(jié)論 VEGF及β3整合素是血管生成過程中重要的調(diào)節(jié)因子。
引用本文： 張聚良,王嶺,晉援朝,王映梅. 肉芽組織血管生成過程中血管內(nèi)皮生長因子及β3整合素的表達. 中國普外基礎(chǔ)與臨床雜志, 2001, 8(6): 378-380. doi: 復(fù)制
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- 1. Folkman J. Angiogenesis in cancer, vascular, rheumatoid and other disease 〔Ｊ〕. Nature Med, 1995; 1(1)∶27.
- 2. Tischer E, Mitchell R, Hartman T, et al. The human gene for vascular endothelial growth factor. Multiple protein forms are encoded through alternative exon splicing 〔Ｊ〕. J Biol Chem, 1991; 266(18)∶11947.
- 3. Fitzgerald LA, Sleiner B, Rall SC, et al. Protein sequence of endothelial glycoprotein derived from a cDNA clone: identify with platelet glycoprotein and similariti to “integrin” 〔Ｊ〕. J Biol Chem, 1987; 262(9)∶3936.
- 4. Chomczynski P, Sacchi N. Singlestep method of RNA isolation by acid guanidinium thiocyanatephenolchloroform extraction 〔Ｊ〕. Anal Biochem, 1987; 162(1)∶ 156.
- 5. Dvorak HF, Brown LF, Detmar M, et al. Vascular permeability factor/vascular endothelial growth factor, microvascular hyperpermeability, and angiogenesis 〔Ｊ〕. Am J Pathol, 1995; 146(5)∶1029.
- 6. Horak ER, Leek R, Klenk N, et al. Angiogenesis, assessed by platelet/endothelial cell adhesion molecule antibodies, as indicator of node metastasis and survival in breast cancer 〔Ｊ〕. Lancet, 1992; 340(8828)∶1120.
- 7. Brooks PC, Clark RA, Cheresh DA. Requirement of vascular integrin alpha v beta 3 for angiogenesis 〔Ｊ〕. Science, 1994; 264(5158)∶569.
- 8. Goede V, Schmidt T, Kimmina S, et al. Analysis of blood vessel maturation processes during cyclic ovarian angiogenesis 〔Ｊ〕. Lab Invest, 1998; 78(11)∶1385.
- 9. Isner JM, Baumgartner I, Rauh G, et al. Treatment of thromboangiitis obliterans (Buergers disease) by intramuscular gene transfer of vascular endothelial growth factor: preliminary clinical results 〔Ｊ〕. J Vasc Surg, 1998; 28(6)∶964.