目的 探討大鼠實驗性急性胰腺炎(AP)模型中胰蛋白酶原激活肽(TAP)的產(chǎn)生及意義。 方法 將90只SD大鼠隨機(jī)分為5組: EP組(3%牛磺膽酸鈉逆行膽胰管注射組); NP組(5%?;悄懰徕c逆行膽胰管注射組); TP組(3%?;悄懰徕c逆行膽胰管注射后半小時經(jīng)股靜脈注入烏司他丁組); CP組(0.9%生理鹽水逆行膽胰管注射組); OP組(假手術(shù)組)。分別于制模后3、6及24小時后處死動物,取血測定血淀粉酶和TAP水平,同時取胰腺標(biāo)本觀察其組織病理學(xué)改變并評分。結(jié)果 NP組胰腺病變明顯重于EP組。制模后3小時和6小時血漿TAP水平NP組分別為(4.798±0.169)nmol/L和(3.999±0.299)nmol/L,明顯高于EP組的(2.416±0.148)nmol/L和(3.356±0.211)nmol/L; 在制模6小時后TP組血漿TAP水平為(1.611±0.113)nmol/L,比EP組的(3.356±0.211)nmol/L明顯降低。EP組與NP組血漿TAP水平差異的出現(xiàn)早于兩組間胰腺組織病理學(xué)改變差異的出現(xiàn)。結(jié)論 血漿TAP水平與大鼠實驗性AP的嚴(yán)重程度有關(guān)。血漿TAP水平可以作為早期預(yù)測實驗性AP嚴(yán)重程度的指標(biāo)。
引用本文: 高軍,張淑文,李非. 胰蛋白酶原激活肽在大鼠實驗性急性胰腺炎中的產(chǎn)生及意義. 中國普外基礎(chǔ)與臨床雜志, 2001, 8(5): 301-303下轉(zhuǎn)306. doi: 復(fù)制
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- 8. Yasuyuki Nakae, Satoru Naruse, Motoji Kitagawa, et al. Activation of trypsinogen in experimental model of acute pancreatitis in rats 〔J〕. Pancreas, 1995; 10(3)∶306.
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