• 1.第三軍醫(yī)大學(xué)西南醫(yī)院普外科(重慶400038);;
  • 2.第三軍醫(yī)大學(xué)西南醫(yī)院病理科;

目的  探討cyclin D1和p53 蛋白在實(shí)驗(yàn)性大鼠同時(shí)性乳腺癌與纖維肉瘤中的表達(dá)及其意義。 方法  通過改良給藥的方法復(fù)制大鼠同時(shí)性乳腺癌與纖維肉瘤動(dòng)物模型,應(yīng)用SP免疫組化技術(shù)檢測(cè)cyclin D1和p53蛋白在乳腺癌、纖維肉瘤及瘤旁組織中的表達(dá)。結(jié)果  在正常乳腺組織中無cyclin D1和p53蛋白表達(dá)，在不典型增生乳腺組織中7/14有cyclin D1表達(dá)，8/18的乳腺癌與9/14的纖維肉瘤中有cyclin D1的表達(dá),7/18的乳腺癌與5/14的纖維肉瘤中有p53蛋白的過度表達(dá); 肉瘤旁組織中無cyclin D1和p53蛋白表達(dá)。cyclin D1和p53蛋白表達(dá)與乳腺癌和纖維肉瘤組織學(xué)分級(jí)有關(guān)，同時(shí)二者的表達(dá)呈負(fù)相關(guān)。結(jié)論  在大鼠同時(shí)性乳腺癌與纖維肉瘤中有cyclin D1和p53蛋白過度表達(dá)，cyclin D1蛋白可能參與腫瘤發(fā)生過程，而p53蛋白表達(dá)與腫瘤的惡性程度相關(guān)。

引用本文： 張毅,姜軍,韓曉蓉,劉麗梅. DMBA誘導(dǎo)大鼠乳腺癌與纖維肉瘤中cyclin D1及p53蛋白的表達(dá)及其意義. 中國(guó)普外基礎(chǔ)與臨床雜志, 2001, 8(5): 285-287. doi: 復(fù)制

1. 華長(zhǎng)江,殷廣福,許正昌.胃腸道多原發(fā)癌的及時(shí)診斷和治療 〔Ｊ〕.實(shí)用癌癥雜志,1994; 9(2)∶115.
2. 姜軍,詹新恩,陳意生等.多原發(fā)性惡性腫瘤模型的建立 〔Ｊ〕. 第三軍醫(yī)大學(xué)學(xué)報(bào),1996; 18(4)∶323.
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4. Russell WO, Cohen J, Enzinger F, et al. A clinical and pathological staging system for soft tissue sarcomas 〔Ｊ〕. Cancer, 1977; 40(7)∶1562.
5. Bito T,Ueda M,Ahmed NU,et al.Cyclin D and retinoblastoma gene product expression in actinic deratosis and cutanious squamous cell carcinoma in relation to p53 expression 〔Ｊ〕. J Cutan Pathol,1998; 22(3)∶427.
6. Lee CC,Yananoto S,Morimura K,et al. Significance of cyclin D1 overexpression in transitional cell carcinomas of the urinary bladder and its correlation with histopathologic features 〔Ｊ〕. Cancer, 1997; 79(4)∶789.
7. Zhang SY,Caamano J,Cooper F,et al.Immunohistochemistry of cyclin D1 in human breast cancer 〔Ｊ〕. Am J Clin Pathol,1994; 102(5)∶695.
8. Naitoh H,Shibata J,Kawaguchi A,et al.Overexpression and localization of cyclin D1 mRNA and antigen in esophageal cancer 〔Ｊ〕. Am J Pathol, 1999; 146(1)∶161.
9. Nishida N,Fukuda Y,Komeda T,et al.Amplification and overexpression of the cyclin D1 gene in aggressive human hepatocellular carcinoma 〔Ｊ〕. Cancer Res,1994; 54(14)∶3107.
10. Betticher DC, Heighway J, Hasleton PS,et al. Prognostic significance of CCND1(cyclin D1) overexpression in primary resected nonsmallcell lung cancer 〔Ｊ〕. Br J Cancer, 1996; 73(4)∶294.
11. Giannino DS,Murphy M,Ruaro EM,et al. Cyclin D1 and p21/war1 are both involved in p53 growth suppression 〔Ｊ〕 . Oncogene 1998; 12(2)∶177.
12. Mansukhani MM,Osborne BM,Zhong JZ,et al.The pattern of p53 and p21waf1/cip1 immunoreactivity in nonHodgkins lymphomas predicts p53 gene status 〔Ｊ〕. Diag Mol Patho, 1997; 6(4)∶222.
13. Worsley SD, Jenmngs BA, Khalil, KH, et al. Cyclin D1 amplification and expression in human breast carcinoma: correlation with histological prognostic markers and oestrogen receptor expression 〔Ｊ〕. J Clin Pathol, 1996; 49(1)∶46.
  1. 1. 華長(zhǎng)江,殷廣福,許正昌.胃腸道多原發(fā)癌的及時(shí)診斷和治療 〔Ｊ〕.實(shí)用癌癥雜志,1994; 9(2)∶115.
  2. 2. 姜軍,詹新恩,陳意生等.多原發(fā)性惡性腫瘤模型的建立 〔Ｊ〕. 第三軍醫(yī)大學(xué)學(xué)報(bào),1996; 18(4)∶323.
  3. 3. 高進(jìn)主編.腫瘤學(xué)基礎(chǔ)與實(shí)驗(yàn) 〔M〕. 第1版. 北京: 人民衛(wèi)生出版社,1999∶410~411.
  4. 4. Russell WO, Cohen J, Enzinger F, et al. A clinical and pathological staging system for soft tissue sarcomas 〔Ｊ〕. Cancer, 1977; 40(7)∶1562.
  5. 5. Bito T,Ueda M,Ahmed NU,et al.Cyclin D and retinoblastoma gene product expression in actinic deratosis and cutanious squamous cell carcinoma in relation to p53 expression 〔Ｊ〕. J Cutan Pathol,1998; 22(3)∶427.
  6. 6. Lee CC,Yananoto S,Morimura K,et al. Significance of cyclin D1 overexpression in transitional cell carcinomas of the urinary bladder and its correlation with histopathologic features 〔Ｊ〕. Cancer, 1997; 79(4)∶789.
  7. 7. Zhang SY,Caamano J,Cooper F,et al.Immunohistochemistry of cyclin D1 in human breast cancer 〔Ｊ〕. Am J Clin Pathol,1994; 102(5)∶695.
  8. 8. Naitoh H,Shibata J,Kawaguchi A,et al.Overexpression and localization of cyclin D1 mRNA and antigen in esophageal cancer 〔Ｊ〕. Am J Pathol, 1999; 146(1)∶161.
  9. 9. Nishida N,Fukuda Y,Komeda T,et al.Amplification and overexpression of the cyclin D1 gene in aggressive human hepatocellular carcinoma 〔Ｊ〕. Cancer Res,1994; 54(14)∶3107.
  10. 10. Betticher DC, Heighway J, Hasleton PS,et al. Prognostic significance of CCND1(cyclin D1) overexpression in primary resected nonsmallcell lung cancer 〔Ｊ〕. Br J Cancer, 1996; 73(4)∶294.
  11. 11. Giannino DS,Murphy M,Ruaro EM,et al. Cyclin D1 and p21/war1 are both involved in p53 growth suppression 〔Ｊ〕 . Oncogene 1998; 12(2)∶177.
  12. 12. Mansukhani MM,Osborne BM,Zhong JZ,et al.The pattern of p53 and p21waf1/cip1 immunoreactivity in nonHodgkins lymphomas predicts p53 gene status 〔Ｊ〕. Diag Mol Patho, 1997; 6(4)∶222.
  13. 13. Worsley SD, Jenmngs BA, Khalil, KH, et al. Cyclin D1 amplification and expression in human breast carcinoma: correlation with histological prognostic markers and oestrogen receptor expression 〔Ｊ〕. J Clin Pathol, 1996; 49(1)∶46.