• 昆明醫(yī)學(xué)院第二附屬醫(yī)院肝膽外科(昆明 650101);

目的  探討一氧化氮（nitric oxide,NO）對(duì)實(shí)驗(yàn)性急性膽道感染大鼠腎功能的影響作用。
方法  Wistar大鼠35只，隨機(jī)分為急性膽道感染組（AC組）、急性膽道感染加左旋精氨酸組（L組）、左旋硝基精氨酸甲基酯組（N組）、單純膽道梗阻對(duì)照組（O組）和假手術(shù)組（SO組）5組，分別測(cè)定血漿NO、BUN、Cr值和腎組織一氧化氮合成酶（NOS）活性，并行肝、腎組織病理變化觀察。
結(jié)果  L組NO、NOS高于其余各組（P＜0.05），BUN、Cr低于AC組和N組（P＜0.05），而與O組比較差異無顯著性意義（P＞0.05），腎組織病理變化較AC組輕； N組NO、NOS低于其余各組，而BUN、Cr則高于其余各組（P＜0.05）。
結(jié)論  NO對(duì)急性膽道感染72小時(shí)大鼠的腎功能有保護(hù)作用，其作用機(jī)理可能與其舒張腎血管，增加血液灌流量等作用有關(guān)。

引用本文： 李智宇,張炳彥,王炳煌,郭永章,張捷. 一氧化氮對(duì)急性膽道感染大鼠腎功能影響作用研究. 中國(guó)普外基礎(chǔ)與臨床雜志, 2001, 8(2): 69-71. doi: 復(fù)制

1. 朱錫光，韓本立.一氧化氮在大鼠急性膽道感染后肝損傷中的作用及其機(jī)理研究 [J].第三軍醫(yī)大學(xué)學(xué)報(bào)，1997； 19（4）∶732.
2. Knowles RG, Merrett M, Salter M, et al. Differential induction of brain, liver and lung nitric oxide synthase by endotoxin in the rat [J]. Biochem J, 1990; 270(3)∶833.
3. 韓本立，龔建平，羅丁.大鼠急性膽道感染動(dòng)物模型的建立 [J].中華實(shí)驗(yàn)外科雜志，1992； 9（1）∶10.
4. Moncade S, Palmer RMJ, Higgs EA. Nitric oxide: physiology, pathophysiology, and pharmacology [J]. Pharmacol Rev, 1991; 43(2)∶109.
5. Inan M, Sayek I, Tel BC, et al. Role of endotoxin and nitric oxide in the pathogenesis of renal failure in obstructive jaundice [J]. Br J Surg, 1997; 84(7)∶943.
6. Haddad IY, Pataki G, Hu P, et al. Quantitation of nitrotyrosine levels in lung sections of patients and animals with acute lung injury [J]. J Clin Chest, 1994; 94(6)∶2407.
7. 王根生，劉耕陶.一氧化氮與肝細(xì)胞損傷 [J].中華醫(yī)學(xué)雜志，1995；75（1）∶57.
8. Endlich K, Muller C, Barthelmebs M, et al. Role of shear stress in nitric oxidedependent modulation of renal angiotensin II vasoconstriction [J]. Br J Pharmacol, 1999; 127(8)∶1929.
9. Olszanecki R, Chlopicki S. Endotoxaemia in rats: role of NO, PAF and TXA2 in pulmonary neutrophil sequestration and hyperlactataemia [J]. J Physiol Pharmacol, 1999; 50(3)∶443.
10. Fierro IM, NascimentoDaSilva V, Arruda MA, et al. Induction of NOS in rat blood PMN in vivo and in vitro： modulation by tyrosine kinase and involvement in bactericidal activity [J]. J Leukoc Biol, 1999; 65(4)∶508.
  1. 1. 朱錫光，韓本立.一氧化氮在大鼠急性膽道感染后肝損傷中的作用及其機(jī)理研究 [J].第三軍醫(yī)大學(xué)學(xué)報(bào)，1997； 19（4）∶732.
  2. 2. Knowles RG, Merrett M, Salter M, et al. Differential induction of brain, liver and lung nitric oxide synthase by endotoxin in the rat [J]. Biochem J, 1990; 270(3)∶833.
  3. 3. 韓本立，龔建平，羅丁.大鼠急性膽道感染動(dòng)物模型的建立 [J].中華實(shí)驗(yàn)外科雜志，1992； 9（1）∶10.
  4. 4. Moncade S, Palmer RMJ, Higgs EA. Nitric oxide: physiology, pathophysiology, and pharmacology [J]. Pharmacol Rev, 1991; 43(2)∶109.
  5. 5. Inan M, Sayek I, Tel BC, et al. Role of endotoxin and nitric oxide in the pathogenesis of renal failure in obstructive jaundice [J]. Br J Surg, 1997; 84(7)∶943.
  6. 6. Haddad IY, Pataki G, Hu P, et al. Quantitation of nitrotyrosine levels in lung sections of patients and animals with acute lung injury [J]. J Clin Chest, 1994; 94(6)∶2407.
  7. 7. 王根生，劉耕陶.一氧化氮與肝細(xì)胞損傷 [J].中華醫(yī)學(xué)雜志，1995；75（1）∶57.
  8. 8. Endlich K, Muller C, Barthelmebs M, et al. Role of shear stress in nitric oxidedependent modulation of renal angiotensin II vasoconstriction [J]. Br J Pharmacol, 1999; 127(8)∶1929.
  9. 9. Olszanecki R, Chlopicki S. Endotoxaemia in rats: role of NO, PAF and TXA2 in pulmonary neutrophil sequestration and hyperlactataemia [J]. J Physiol Pharmacol, 1999; 50(3)∶443.
  10. 10. Fierro IM, NascimentoDaSilva V, Arruda MA, et al. Induction of NOS in rat blood PMN in vivo and in vitro： modulation by tyrosine kinase and involvement in bactericidal activity [J]. J Leukoc Biol, 1999; 65(4)∶508.