目的 探討端粒酶作為大腸癌診斷、治療和預(yù)后參數(shù)的價(jià)值。
方法 應(yīng)用PCR-TRAP-ELISA方法，檢測(cè)大腸癌、癌旁和正常大腸粘膜組織的端粒酶活性表達(dá)。
結(jié)果 端粒酶在大腸癌、癌旁和正常大腸粘膜組織中表達(dá)的陽(yáng)性率分別為84.8%(39/46)、20.0%(6/30)和0(0/20)，大腸癌組織中端粒酶表達(dá)陽(yáng)性率明顯高于癌旁和正常大腸粘膜(P＜0.001)。早期大腸癌(Dukes A期)即有66.7%者存在端粒酶活化。腫瘤組織學(xué)分級(jí)越低，端粒酶表達(dá)陽(yáng)性率越高(P＜0.05)。
結(jié)論 端粒酶可能是大腸癌惡性浸潤(rùn)的早期事件，可作為診斷大腸癌的一個(gè)有用的輔助指標(biāo)，并有助于預(yù)測(cè)預(yù)后和指導(dǎo)治療方案的選擇。
引用本文： 駱成玉,趙丹寧,李世擁,祝學(xué)光. 檢測(cè)大腸癌組織中端粒酶活性的臨床意義. 中國(guó)普外基礎(chǔ)與臨床雜志, 2001, 8(1): 18-20. doi: 復(fù)制
| 1. | Hoos A, Heep HH, Kaul S, et al. Telomerase activity correlates with tumour aggressiveness and reflects therapy effect in breast cancer [J]. Int J Cancer, 1998; 79(1)∶8. |
| 2. | Fang DC, Yong J, Luo YH, et al. Detection of temolerase activity in biopsy samples of colorectal cancer [J]. J Gastroenterol Hepatol, 1999; 14(4)∶328. |
| 3. | Kim NW, Pieatyszek MA, Prowse KR, et al. Specific association of human telomerase activity with immortal cells and cancer [J]. Science, 1994; 266(5232)∶2011. |
| 4. | Fujita M, Tomita S, Fukui H, et al. Semiquantitative procedure for telomeric repeat amplification protocol(TRAP) assay in colorectal carcinomas [J]. J Exp Clin Cancer Res, 1999; 18(1)∶119. |
| 5. | Shay JW, Wright WE. The reactivation of telomerase activity in cancer progression [J]. Trends Genet, 1996; 12(1)∶129. |
| 6. | Albanell J, Han W, Mellado B, et al. Telomerase activity is repressed during differentiation of maturationsensitive but not resistant human tumor cell lines [J]. Cancer Res, 1996; 56(7)∶1503. |
- 1. Hoos A, Heep HH, Kaul S, et al. Telomerase activity correlates with tumour aggressiveness and reflects therapy effect in breast cancer [J]. Int J Cancer, 1998; 79(1)∶8.
- 2. Fang DC, Yong J, Luo YH, et al. Detection of temolerase activity in biopsy samples of colorectal cancer [J]. J Gastroenterol Hepatol, 1999; 14(4)∶328.
- 3. Kim NW, Pieatyszek MA, Prowse KR, et al. Specific association of human telomerase activity with immortal cells and cancer [J]. Science, 1994; 266(5232)∶2011.
- 4. Fujita M, Tomita S, Fukui H, et al. Semiquantitative procedure for telomeric repeat amplification protocol(TRAP) assay in colorectal carcinomas [J]. J Exp Clin Cancer Res, 1999; 18(1)∶119.
- 5. Shay JW, Wright WE. The reactivation of telomerase activity in cancer progression [J]. Trends Genet, 1996; 12(1)∶129.
- 6. Albanell J, Han W, Mellado B, et al. Telomerase activity is repressed during differentiation of maturationsensitive but not resistant human tumor cell lines [J]. Cancer Res, 1996; 56(7)∶1503.