• 成都軍區(qū)總醫(yī)院普通外科中心（成都610083）;

目的 探討新城疫病毒（newcastle disease virus，NDV）對腫瘤浸潤淋巴細胞（tumor infiltrating lymphocyte，TIL）的作用。方法將6例原發(fā)性肝癌癌塊進行處理，分離出TIL，每例等分成3份，分別加入HA效價為1∶160 NDV液20 μl（NDV組）、終濃度為1000 u/ml的rIL2（rIL2組）和0.9%的生理鹽水20 μl（對照組），置37℃、5% CO2恒溫箱中培養(yǎng)。以流式細胞儀計數(shù)CD3、CD4和CD8細胞； 用ELISA法檢測NDV組和對照組上清液IL2、IFNγ和TNFβ含量。結果配對資料顯示，NDV組CD3、CD4明顯高于對照組(P＜0.01)和rIL2組（P＜0.01，P＜0.05），3組CD8無明顯差異； NDV組IL2、IFNγ和TNFβ明顯高于對照組（分別為P＜0.01,P＜0.01，P＜0.05）。結論NDV對TIL有直接激活作用。

引用本文： 趙鐵軍,陳濤,田伏洲,李小軍,尹志良. 新城疫病毒對腫瘤浸潤淋巴細胞的作用. 中國普外基礎與臨床雜志, 2002, 9(5): 329-331. doi: 復制

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  2. 2. Schirrmacher V, Haas C, Bonifer R, et al. Viros potentiation of tumor vaccine Tcell stimulatory capacity requires cell surface binding but not infection [J]. Clin Cancer Res, 1997; 3(7)∶1135.
  3. 3. Haas C, Ertel C, Gerhards R, et al. Introduction of adhesive and costimulatory immune functions into tumor cells by infection with Newcastle Disease Virus [J]. Int J Oncol,1998; 13(6)∶1105.
  4. 4. Kenney S，Pagano JS. Viruses as oncolytic agents: a new age for “therapeutic” viruses？ [J]. J Natl Cancer Inst, 1994; 86(16)∶1185.
  5. 5. Yeow WS, Beilharz MW，Lai CM. The in vitro expression patterns of individual type I interferon genes in Newcastle disease virus infected murine splenocytes and fibroblasts [J]. Int J Biochem Cell Biol,1997; 29(3)∶513.
  6. 6. Weidmann E, Trucco M， Whiteside TL. Relevance of the T cell receptor for immunotherapy of cancer [J]. Cancer Immunol Immunother, 1994; 39(1)∶1.
  7. 7. Kagi D, Seiler P, Pavlovic J, et al. The roles of perforin and Fasdependent cytotoxicity in protection against cytopathic and noncytopathic viruses [J]. Eur J Immunol, 1995; 25(10)∶3256.
  8. 8. Bradley LM, Yoshimoto K，Swain SL. The cytokines IL4, IFNgamma, and IL12 regulate the development of subsets of memory effector helper T cells in vitro [J]. J Immunol, 1995; 155(4)∶1713.
  9. 9. Noble A, Macary PA，Kemeny DM. IFNgamma and IL4 regulate the growth and differentiation of CD8+ T cells into subpopulations with distinct cytokine profiles [J]. J Immunol, 1995; 155(6)∶2928.