• 1.吉林大學(xué)第一醫(yī)院普通外科（長(zhǎng)春130021）;;
  • 2.內(nèi)蒙古民族大學(xué)附屬醫(yī)院（通遼028000）;;
  • 3.吉林大學(xué)第二醫(yī)院（長(zhǎng)春130021）;

目的研究抑凋亡基因bcl2和促凋亡基因bax在大腸癌及癌旁組織中的表達(dá)及意義。方法應(yīng)用免疫組織化學(xué)SP法對(duì)31例大腸癌患者的癌組織及距癌組織外緣3 cm內(nèi)的癌旁組織和3 cm以遠(yuǎn)的正常大腸組織中bcl2和bax基因的表達(dá)進(jìn)行檢測(cè)。結(jié)果大腸癌及其癌旁組織中bcl2蛋白表達(dá)陽性率分別為64.5%和60.0%，顯著高于正常組織（35.0%），P＜0.05； bax蛋白表達(dá)陽性率分別為45.2%和45.0%，顯著低于正常組織（60.0%），P＜0.05； 大腸癌組織與癌旁組織中bcl2和bax蛋白表達(dá)陽性率的差異無顯著性意義（P gt;0.05）。bcl2和bax蛋白在大腸癌組織中的表達(dá)與其病理類型和臨床分期無明顯關(guān)系（P gt;0.05）。結(jié)論大腸癌及其癌旁組織中有不同水平的bcl2蛋白過度表達(dá)和bax蛋白低表達(dá)，兩者通過參與調(diào)節(jié)細(xì)胞凋亡而與大腸癌的發(fā)生有關(guān)，但與大腸癌的病理類型、臨床分期無關(guān)。距大腸癌組織外緣3 cm以內(nèi)的癌旁組織應(yīng)視為癌前期組織，手術(shù)時(shí)應(yīng)切除。

引用本文： 王舉,竇忠霞,韓喜春,程雷,黃大鶴. 大腸癌及癌旁組織中抑凋亡基因bcl2和促凋亡基因bax的表達(dá)及意義. 中國(guó)普外基礎(chǔ)與臨床雜志, 2002, 9(4): 252-254. doi: 復(fù)制

版權(quán)信息： ?四川大學(xué)華西醫(yī)院華西期刊社《中國(guó)普外基礎(chǔ)與臨床雜志》版權(quán)所有，未經(jīng)授權(quán)不得轉(zhuǎn)載、改編

1. Valentini AM, Caruso ML, Armentano R, et al. Programmed cell death in colorectal carcinogenesis [J]. Anticancer Res, 1999; 19(4B)∶3019.
2. Feuerhake F, Unterberger P, Hofter EA. Cell turnover in apocrine metaplasia of the human mammary gland epithelium: apoptosis, proliferation, and immunohistochemical detection of bcl2, bax, EGFR, and cerb B2 gene products [J]. Acta Histochem, 2001; 103(1)∶53.
3. Sinicrope FA, Ruan SB, Cleary KR, et al. bcl2 and p53 oncoprotein expression during colorectal tumorigenesis [J]. Cancer Res, 1995; 55(2)∶237.
4. Jansson DS, Radosevich JA, Carney WP, et al. An immunohistochemical analysis of ras oncogene expression in epithelial noeplasms of the colon [J]. Cancer, 1990; 65(6)∶1329.
5. Wang Q, Gao H, Chen Y, et al. Biopathologic characteristics of DNA content in crypt cells of transitional mucosa adjacent to carcinomas of the rectum and rectosigmoid [J]. Dis Colon Rectum, 1992; 35(7)∶670.
  1. 1. Valentini AM, Caruso ML, Armentano R, et al. Programmed cell death in colorectal carcinogenesis [J]. Anticancer Res, 1999; 19(4B)∶3019.
  2. 2. Feuerhake F, Unterberger P, Hofter EA. Cell turnover in apocrine metaplasia of the human mammary gland epithelium: apoptosis, proliferation, and immunohistochemical detection of bcl2, bax, EGFR, and cerb B2 gene products [J]. Acta Histochem, 2001; 103(1)∶53.
  3. 3. Sinicrope FA, Ruan SB, Cleary KR, et al. bcl2 and p53 oncoprotein expression during colorectal tumorigenesis [J]. Cancer Res, 1995; 55(2)∶237.
  4. 4. Jansson DS, Radosevich JA, Carney WP, et al. An immunohistochemical analysis of ras oncogene expression in epithelial noeplasms of the colon [J]. Cancer, 1990; 65(6)∶1329.
  5. 5. Wang Q, Gao H, Chen Y, et al. Biopathologic characteristics of DNA content in crypt cells of transitional mucosa adjacent to carcinomas of the rectum and rectosigmoid [J]. Dis Colon Rectum, 1992; 35(7)∶670.