目的探討自主神經(jīng)控制對(duì)人肝細(xì)胞增殖的影響及其機(jī)理，并對(duì)增殖相關(guān)受體定位。方法用去甲腎上腺素（NE）及其拮抗劑及激動(dòng)劑和乙酰膽堿（Ach）及其阻滯劑作用于人傳代正常肝L02細(xì)胞及肝癌Bel7402細(xì)胞，分別于4 h、24 h、48 h及72 h用改良MTT法檢測(cè)上述藥物對(duì)細(xì)胞增殖的影響； 免疫組織化學(xué)染色法對(duì)肝L02細(xì)胞的腎上腺素受體（α1BAR及β2AR）和表皮生長(zhǎng)因子受體（EGFR）定位。結(jié)果NE對(duì)人正常肝細(xì)胞及肝癌細(xì)胞具有促增殖作用，且隨濃度增加其促增殖作用也增加，4 h的增殖率較其它時(shí)間點(diǎn)顯著增高（P＜0.05）； NE+心得安組的作用與NE組間差異無顯著性意義； 二羥苯基異丙氨基乙醇的作用不明顯; Ach對(duì)人正常肝細(xì)胞的增殖具有抑制作用，且隨濃度增加其抑制作用也增加，加用阿托品在24 h及48 h與Ach比抑制率顯著降低（P＜0.05）； 琥珀膽堿組在24 h、48 h及72 h對(duì)細(xì)胞的增殖也具有抑制作用（P＜0.05，P＜0.01）。所有人肝L02細(xì)胞都呈α1BAR、β2AR及EGFR免疫反應(yīng)陽性，主要定位于胞膜及胞漿。結(jié)論血清存在下，交感神經(jīng)遞質(zhì)NE早期對(duì)人正常肝細(xì)胞L02及人肝癌細(xì)胞Bel7402具有促增殖作用，其作用是通過激動(dòng)αAR實(shí)現(xiàn)的, βAR對(duì)人肝細(xì)胞增殖無影響。副交感神經(jīng)遞質(zhì)Ach對(duì)人正常肝細(xì)胞L02的增殖具有抑制作用，其作用是通過激動(dòng)M及N型膽堿受體實(shí)現(xiàn)的。
引用本文： 李立,陳剛,郭永章. 神經(jīng)遞質(zhì)對(duì)肝細(xì)胞增殖的影響△. 中國(guó)普外基礎(chǔ)與臨床雜志, 2002, 9(4): 238-241. doi: 復(fù)制
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- 3. Kajiyama Y, Ui M. Differential mitogenic actions of alpha 1 and betaadrenergic agonists on rat hepatocyte [Ｊ]. Cell Signal,1998; 10(4)∶241.
- 4. Kimura M, Ogihara M. Proliferation of adult rat hepatocytes by hepatocyte growth factor is potentiated by both phenylephrine and metaproterenol [Ｊ]. J Pharmacol Exp Ther,1997; 282(3)∶ 1146.
- 5. Elaeva NL, Stroikov IuN. Mechanism of action of eserine on DNA synthesis in rat liver [Ｊ]. Biull Eksp Biol Med, 1979; 87(1)∶24.
- 6. Kiba T, Saito S, Numata K,et al. Expresson of apoptosis on rat liver by hepatic vagus hyperactivity after ventromedial hypothalamic lesioning [Ｊ]. Am J Physiol Gastrointest Liver Physiol，2001; 280(5)∶G958.
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