• 1.福建省立醫(yī)院腫瘤科（福州350001）;;
  • 2.上海第二醫(yī)科大學(xué)附屬瑞金醫(yī)院（上海200025）;

目的  探討腫瘤新生血管形成在大腸癌進展中的作用。
方法  采用鼠抗人第八因子相關(guān)抗原單克隆抗體的免疫組織化學(xué)ABC法，對102例大腸癌手術(shù)切除標(biāo)本中微血管進行定量檢測。計數(shù)200倍視野下3個最高血管密度區(qū)的微血管數(shù)（MVC），取其均數(shù)，分析MVC與大腸癌臨床病理因素及預(yù)后之間的關(guān)系。
結(jié)果  MVC與大腸癌Dukes分期、淋巴結(jié)轉(zhuǎn)移及肝轉(zhuǎn)移有關(guān)（P＜0.01）； 高MVC組（MVC≥14個）大腸癌術(shù)后復(fù)發(fā)率（60.4%）顯著高于低MVC組（MVC＜14個）的復(fù)發(fā)率（26.5%），P＜0.01，高MVC患者預(yù)后比低MVC患者差（P＜0.01）。
結(jié)論  大腸癌組織內(nèi)的MVC是預(yù)測腫瘤惡性生物學(xué)行為的一個有用指標(biāo)，可用于識別有高危復(fù)發(fā)和不良預(yù)后的病例。

引用本文： 雷雯,郁寶銘,周錫庚. 大腸癌組織中微血管定量檢測的臨床價值. 中國普外基礎(chǔ)與臨床雜志, 2002, 9(1): 40-42. doi: 復(fù)制

1. Norton JA. Tumor angiogenesis [Ｊ]. Ann Surg, 1995; 222（6）∶693.
2. Mooteri H, Rubin D, Leurgans S, et al. Tumor angiogenesis in primary and metastatic colorectal cancers [Ｊ]. Dis Colon Rectum, 1996; 39（10）∶1073.
3. Weidner N, Folkman J, Pozza F, et al. Tumor angiogenesis: a new significant and independent prognostic indicator in earlystage breast carcinoma [Ｊ]. J Natl Cancer Inst,1992; 84（8）∶1875.
4. Folkman J. Tumor angiogenesis and tissue factor [Ｊ]. Nature Medicine, 1996; 2（1）∶167.
5. Banner BF, Whitehouse R, Baker SP, et al. Tumor angiogenesis in stage II colorectal carcinoma association with survival [Ｊ]. Am J Clin Pathol, 1998; 109（6）∶733.
6. Maeda K ,Chung YS, Ogawa Y, et al. Prognostic value of vascular endothelial growth factor expression in gastric carcinoma [Ｊ]. Cancer, 1996; 77（5）∶858.
7. Weidner N, Carroll PR, Flax J, et al. Tumor angiogenesis correlates with metastasis in invasive prostate carcinoma [Ｊ]. Am J Pathol, 1993; 143（2）∶401.
8. Detmar M. Molecular regulation of angiogenesis in skin [Ｊ]. J Invest Dermatol, 1996; 106（1）∶207.
9. Abdulrauf SI, Edvardsen K, Ho KL, et al. Vascular endothelial growth factor expression and vascular density as prognostic markers of survival in patients with lowgrade astrocytoma [Ｊ]. J Neurosurg,1998; 88（3）∶513.
10. Volm M, Koomagi R, Kaufmann M, et al. Microvessel density, expression of protooncogenes, resistancerelated proteins and incidence of metastasis in primary ovarian carcinomas [Ｊ]. Clin Exp Metastasis, 1996; 14（2）∶209.
11. Tomisaki S, Ohno S, Ichiyoshi Y, et al. Microvessel quantification and its possible relation with liver metastasis in colorectal cancer [Ｊ]. Cancer, 1996; 77（8 Suppl)∶1722.
12. Bossi P,Viale G,Lee AK,et al.Angiogenesis in colorectal tumors: microvessel quantitation in adenomas and carcinomas with clinicopathological correlations [Ｊ]. Cancer Res, 1995; 55（21）∶5049.
13. Frank RE, Saclarides TJ, Leurgans S, et al. Tumor angiogenesis as a predictor of recurrence and survival in patients with nodenegative colon cancer [Ｊ]. Ann Surg, 1995; 222（6）∶695.
14. Engel CJ, Bennett ST, Chambers AF, et al. Tumor angiogenesis predicts recurrence in invasive colorectal cancer when controlled for dukes staging [Ｊ]. Am J Surg Pathol, 1996; 20（10）∶1260.
15. Saclarides TJ, Speziale NJ, Drab E, et al. Tumor angiogenesis and rectal carcinoma [Ｊ]. Dis Colon Rectum,1994; 37（5）∶921.
16. Takebayashi Y, Akiyama S, Yamada K, et al. Angiogenesis as an unfavorable prognostic factor in human colorectal carcinoma [Ｊ]. Cancer, 1996; 78（2）∶226.
17. Tanigawa N, Amaya H, Matsumura M, et al. Tumor angiogenesis and mode of metastasis in patients with colorectal cancer [Ｊ]. Cancer Res,1997; 57（6）∶1043.
18. Nanashima A, Ito M, Sekine I, et al. Significance of angiogenic factors in liver metastatic tumors originating from colorectal cancers [Ｊ]. Dig Dis Sci, 1998; 43（12）∶2634.
  1. 1. Norton JA. Tumor angiogenesis [Ｊ]. Ann Surg, 1995; 222（6）∶693.
  2. 2. Mooteri H, Rubin D, Leurgans S, et al. Tumor angiogenesis in primary and metastatic colorectal cancers [Ｊ]. Dis Colon Rectum, 1996; 39（10）∶1073.
  3. 3. Weidner N, Folkman J, Pozza F, et al. Tumor angiogenesis: a new significant and independent prognostic indicator in earlystage breast carcinoma [Ｊ]. J Natl Cancer Inst,1992; 84（8）∶1875.
  4. 4. Folkman J. Tumor angiogenesis and tissue factor [Ｊ]. Nature Medicine, 1996; 2（1）∶167.
  5. 5. Banner BF, Whitehouse R, Baker SP, et al. Tumor angiogenesis in stage II colorectal carcinoma association with survival [Ｊ]. Am J Clin Pathol, 1998; 109（6）∶733.
  6. 6. Maeda K ,Chung YS, Ogawa Y, et al. Prognostic value of vascular endothelial growth factor expression in gastric carcinoma [Ｊ]. Cancer, 1996; 77（5）∶858.
  7. 7. Weidner N, Carroll PR, Flax J, et al. Tumor angiogenesis correlates with metastasis in invasive prostate carcinoma [Ｊ]. Am J Pathol, 1993; 143（2）∶401.
  8. 8. Detmar M. Molecular regulation of angiogenesis in skin [Ｊ]. J Invest Dermatol, 1996; 106（1）∶207.
  9. 9. Abdulrauf SI, Edvardsen K, Ho KL, et al. Vascular endothelial growth factor expression and vascular density as prognostic markers of survival in patients with lowgrade astrocytoma [Ｊ]. J Neurosurg,1998; 88（3）∶513.
  10. 10. Volm M, Koomagi R, Kaufmann M, et al. Microvessel density, expression of protooncogenes, resistancerelated proteins and incidence of metastasis in primary ovarian carcinomas [Ｊ]. Clin Exp Metastasis, 1996; 14（2）∶209.
  11. 11. Tomisaki S, Ohno S, Ichiyoshi Y, et al. Microvessel quantification and its possible relation with liver metastasis in colorectal cancer [Ｊ]. Cancer, 1996; 77（8 Suppl)∶1722.
  12. 12. Bossi P,Viale G,Lee AK,et al.Angiogenesis in colorectal tumors: microvessel quantitation in adenomas and carcinomas with clinicopathological correlations [Ｊ]. Cancer Res, 1995; 55（21）∶5049.
  13. 13. Frank RE, Saclarides TJ, Leurgans S, et al. Tumor angiogenesis as a predictor of recurrence and survival in patients with nodenegative colon cancer [Ｊ]. Ann Surg, 1995; 222（6）∶695.
  14. 14. Engel CJ, Bennett ST, Chambers AF, et al. Tumor angiogenesis predicts recurrence in invasive colorectal cancer when controlled for dukes staging [Ｊ]. Am J Surg Pathol, 1996; 20（10）∶1260.
  15. 15. Saclarides TJ, Speziale NJ, Drab E, et al. Tumor angiogenesis and rectal carcinoma [Ｊ]. Dis Colon Rectum,1994; 37（5）∶921.
  16. 16. Takebayashi Y, Akiyama S, Yamada K, et al. Angiogenesis as an unfavorable prognostic factor in human colorectal carcinoma [Ｊ]. Cancer, 1996; 78（2）∶226.
  17. 17. Tanigawa N, Amaya H, Matsumura M, et al. Tumor angiogenesis and mode of metastasis in patients with colorectal cancer [Ｊ]. Cancer Res,1997; 57（6）∶1043.
  18. 18. Nanashima A, Ito M, Sekine I, et al. Significance of angiogenic factors in liver metastatic tumors originating from colorectal cancers [Ｊ]. Dig Dis Sci, 1998; 43（12）∶2634.