目的探討鼠肝大部切除術(shù)后空、回腸粘膜固有層細(xì)胞免疫功能的變化及其與腸道細(xì)菌移位的關(guān)系。方法將48只SD大鼠隨機(jī)分為實(shí)驗(yàn)組和假手術(shù)組，每組24只。實(shí)驗(yàn)組切除70%肝臟，假手術(shù)組除不切除肝臟外，其余手術(shù)步驟同實(shí)驗(yàn)組。分別于術(shù)后6、12、24和72 h取兩組大鼠（n=6）空、回腸粘膜冰凍切片，而后行免疫組化染色，觀察不同時(shí)相腸粘膜固有層CD3+、CD4+、CD8+ T淋巴細(xì)胞的數(shù)量及肝臟功能的變化。結(jié)果實(shí)驗(yàn)組術(shù)后24 h和72 h，其腸粘膜固有層CD3+、CD4+和CD8+T淋巴細(xì)胞的數(shù)量較假手術(shù)組明顯下降（P＜0.05），而兩組大鼠術(shù)后不同時(shí)相的ALT及AST變化，實(shí)驗(yàn)組明顯高于假手術(shù)組（P lt;0.05）。結(jié)論鼠肝大部切除（70%）術(shù)后24 h，腸粘膜固有層CD3+、CD4+和CD8+ T淋巴細(xì)胞數(shù)量明顯降低。這種細(xì)胞免疫功能降低所導(dǎo)致的腸粘膜屏障功能受損，可能是造成腸道細(xì)菌移位的原因之一。
引用本文： 楊之斌,張炳彥,朱洪,王炳煌,郭永章. 鼠肝大部切除術(shù)后腸粘膜細(xì)胞免疫功能的變化及其與腸道細(xì)菌移位的關(guān)系. 中國(guó)普外基礎(chǔ)與臨床雜志, 2003, 10(5): 469-470. doi: 復(fù)制
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