目的 探討5-氟尿嘧啶（5-fluorouracil,5-FU）體外誘導(dǎo)直腸癌HR8348細(xì)胞凋亡的作用及細(xì)胞凋亡與bcl-2、bcl-xl、bax及p53表達(dá)的關(guān)系。方法 經(jīng)5-FU處理HR8348細(xì)胞24 h后，用甲基綠-派若寧染色法和TUNEL法檢測(cè)細(xì)胞凋亡情況，并用SP免疫組織化學(xué)法檢測(cè)HR8348細(xì)胞中bcl-2、bcl-xl、bax和p53的表達(dá)。結(jié)果 HR8348細(xì)胞經(jīng)5-FU作用24 h后，細(xì)胞凋亡指數(shù)（AI）明顯高于對(duì)照組，差異有顯著性意義（P＜0.01）。5-FU作用不同時(shí)相bcl-2的表達(dá)評(píng)分結(jié)果與對(duì)照組比較差異均無(wú)顯著性意義； 不同時(shí)相bcl-xl的表達(dá)評(píng)分結(jié)果與對(duì)照組比較稍降低； 而bax的表達(dá)評(píng)分結(jié)果隨時(shí)間延長(zhǎng)明顯增加，24 h、36 h的表達(dá)評(píng)分結(jié)果明顯高于對(duì)照組（P＜0.01），而p53在5-FU組和對(duì)照組各時(shí)相均未見表達(dá)。結(jié)論 5-FU具有誘導(dǎo)HR8348細(xì)胞凋亡的作用； 5-FU可能通過(guò)上調(diào)bax的表達(dá)并改變bax/bcl-xl的比值而誘導(dǎo)HR8348細(xì)胞凋亡。
引用本文： 季風(fēng)存,程若川,康俊升. 5-氟尿嘧啶誘導(dǎo)直腸癌HR8348細(xì)胞凋亡作用的研究. 中國(guó)普外基礎(chǔ)與臨床雜志, 2003, 10(6): 564-567. doi: 復(fù)制
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- 2. Sinicrope FA,Ruan SB,Cleary KR,et al. bcl2 and p53 oncoprotein expression during colorectal tumorigenesis [Ｊ].Cancer Res, 1995; 55(2)∶237.
- 3. 郁寶銘， 鄭民華， 王灝， 等. p53基因在5氟尿嘧啶致大腸腫瘤細(xì)胞凋亡的影響 [Ｊ]. 中國(guó)癌癥雜志, 1998; 8（4）∶243.
- 4. Koshiji M,Adachi Y,Taketani S,et al.Mechanisms underlying apoptosis induced by combination of 5fluorouracil and interferongamma [Ｊ].Biochem Biophys Res Commun, 1997; 240(2)∶376.
- 5. 駱成玉， 李世擁. 大腸癌bcl2基因表達(dá)和重排的研究 [Ｊ]. 中國(guó)普外基礎(chǔ)與臨床雜志， 1999； 6（1）∶4.
- 6. Yang E,Korsmeyer SJ.Molecular thanatopsis: a discourse on the bcl2 family and cell death [Ｊ].Blood, 1996; 88(2)∶386.
- 7. Huang DC,Cory S, Strasser A. bcl2,bclxl and adenovirus protein EIB19kD are functionally equivalent in their ability to inhibit cell death [Ｊ].Oncogene, 1997; 14(4)∶405.
- 8. Sedlak TW,Oltvai ZN,Yang E,et al.Multiple bcl2 family members demonstrate selective dimerization with bax [Ｊ].Proc Natl Acad Sci USA， 1995； 92(17)∶7834.
- 9. Nita ME,Nagawa H, Tominaga O,et al.5Fluorouracil induces apoptosis in human colon cancer cell lines with modulation of bcl2 family proteins [Ｊ]. Br J Cancer， 1998; 78(8)∶986.
- 10. Schneider HJ,Sampson SA,Cunningham D,et al. bcl2 expression and response to chemotherapy in colorectal adenocarcimomas [Ｊ].Br J Cancer, 1997; 75(3)∶427.
- 11. Waston AJ,Merritt AJ,Jones LS, et al.Evidence for reciprocity of bcl2 and p53 expression in human colorectal adenomas and carcinomas [Ｊ].Br J Cancer, 1996; 73(8)∶889.
- 12. Soussi T.The p53 tumor suppressor gene: from molecular biology to clinical investigation [Ｊ]. Ann N Y Acad Sci, 2000; 910∶121.
- 13. Silvestrini R,Veneroni S,Daidone MG,et al.The bcl2 protein:a prognostic indicator strongly related to p53 protein in lymph nodenegative breast cancer patients [Ｊ].J Natl Cancer Inst, 1994; 86(7)∶499.