• 四川大學(xué)華西醫(yī)院普外科（成都610041）;

目的  研究誘導(dǎo)型一氧化氮合酶（iNOS）和p53蛋白在肝細胞癌中的表達及其與腫瘤血管形成的關(guān)系。
方法  采用免疫組化和圖像分析技術(shù)檢測59例肝細胞癌患者腫瘤組織中iNOS和p53蛋白的表達，CD34單克隆抗體免疫組化染色檢測腫瘤組織微血管密度（MVD）。
結(jié)果  ①iNOS和p53蛋白在肝細胞癌組織中表達陽性率分別為81.4%（48/59）和64.4%（38/59），表達強度（IOD值）分別為5 635±1 287和3 352±873。②MVD為32.5±2.73，以腫瘤邊緣和癌旁組織微血管較密集。③iNOS的表達與p53蛋白表達呈顯著正相關(guān)（r=0.65, P＜0.05）； iNOS的表達與MVD呈顯著正相關(guān)（r=0.75, P＜0.05）； p53蛋白的表達與MVD呈顯著正相關(guān)（r=0.72, P＜0.05）。
結(jié)論  肝細胞癌組織中存在iNOS和p53蛋白的高表達； iNOS和p53可促進肝癌血管形成。

引用本文： 張劍,何生,李茂德. 肝細胞癌中iNOS和p53蛋白的表達及其與腫瘤血管形成的關(guān)系. 中國普外基礎(chǔ)與臨床雜志, 2004, 11(5): 396-398. doi: 復(fù)制

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  1. 1. Papapetropoulos A, GarciaCardena G, Madri JA, et al. Nitric oxide production contributes to the angiogenic properties of vascular endothelial growth factor in human endothelial cells [Ｊ]. J Clin Invest, 1997; 100(12)∶3131.
  2. 2. Mukhopadhyay D, Tsiokas L, Sukhatme VP. Wildtype p53 and vSrc exert opposing influences on human vascular endothelial growth factor gene expression [Ｊ]. Cancer Res, 1995; 55(24)∶6161.
  3. 3. Mattern J, Koomagi R, Volm M. Biological characterization of subgroups of squamous cell lung carcinomas [Ｊ]. Clin Cancer Res, 1999; 5(6)∶1459.
  4. 4. Weidner N, Folkman J, Pozza F, et al. Tumor angiogenesis: a new significant and independent prognostic indicator in earlystage breast carcinoma [Ｊ]. J Natl Cancer Inst, 1992; 84(24)∶1875.
  5. 5. Rosbe KW, Prazma J, Petrusz P, et al. Immunohistochemical characterization of nitric oxide synthase activity in squamous cell carcinoma of the head and neck [Ｊ]. Otolaryngol Head Neck Surg, 1995; 113(5)∶541.
  6. 6. Thomsen LL, Lawton FG, Knowles RG, et al. Nitric oxide synthase activity in human gynecological cancer [Ｊ]. Cancer Res, 1994; 54(5)∶1352.
  7. 7. Fujimoto H, Ando Y, Yamashita T, et al. Nitric oxide synthase activity in human lung cancer [Ｊ]. Jpn J Cancer Res, 1997; 88(12)∶1190.
  8. 8. Parenti A, Morbidelli L, Cui XL, et al. Nitric oxide is an upstream signal of vascular endothelial growth factorinduced extracellular signalregulated kinase1/2 activation in postcapillary endothelium [Ｊ]. J Biol Chem, 1998; 273(7)∶4220.
  9. 9. Chin K, Kurashima Y, Ogura T, et al. Induction of vascular endothelial growth factor by nitric oxide in human glioblastoma and hepatocellular carcinoma cells [Ｊ]. Oncogene, 1997; 15(4)∶437.