目的建立一種穩(wěn)定的急性壞死性胰腺炎模型。方法36只雄性ICR小鼠用查隨機(jī)數(shù)字表的方法隨機(jī)分為對照組（n=6）和實(shí)驗(yàn)組（n=30）。實(shí)驗(yàn)組予以雨蛙素腹腔注射（50 μg/kg），每小時(shí)注射1次，共7次，并分別于首次注射后9 h、18 h、24 h、48 h和72 h各處死6只； 對照組予以腹腔注射等量的生理鹽水，于注射后18 h處死。比較兩組動(dòng)物的血淀粉酶值、胰腺重量及胰腺組織學(xué)評(píng)分分值。結(jié)果腹腔注射雨蛙素后9 h，小鼠血淀粉酶值升高，胰腺重量增加，18 h達(dá)峰值，24 h后逐漸下降。二者與胰腺病理損害程度相一致。18 h胰腺間質(zhì)炎性細(xì)胞浸潤和腺泡細(xì)胞壞死明顯，胰腺組織學(xué)評(píng)分值達(dá)到最大值（P＜0.05）。結(jié)論大劑量雨蛙素腹腔注射能誘導(dǎo)ICR小鼠產(chǎn)生急性壞死性胰腺炎。該模型制備方法簡單，無創(chuàng)傷性，模型穩(wěn)定，重復(fù)性好。
引用本文： 李全生,陳曉理,周總光,劉續(xù)寶,張肇達(dá). 雨蛙素腹腔注射法建立小鼠急性壞死性胰腺炎模型的實(shí)驗(yàn)研究. 中國普外基礎(chǔ)與臨床雜志, 2004, 11(4): 335-337. doi: 復(fù)制
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- 1. Niederau C, Ferrell LD, Grendell JH. Caeruleininduced acute necrotizing pancreatitis in mice: protective effects of proglumide, benzotript, and secretin [Ｊ]. Gastroenterology， 1985; 88(5 Pt 1)∶1192.
- 2. van Laethem JL, Eskinazi R, Louis H, et al. Multisystemic production of interleukin 10 limits the severity of acute pancreatitis in mice [Ｊ]. Gut, 1998; 43(3)∶408.
- 3. 劉興，陳懷仁，楊得同，等. 蛙皮素致小鼠急性壞死性胰腺炎模型 [Ｊ]. 南京鐵道醫(yī)學(xué)院學(xué)報(bào)，1996； 15（4）∶286.
- 4. 薛建國，王宇，許元弟. 建立大鼠急性出血壞死性胰腺炎模型方法的改進(jìn) [Ｊ]. 中華實(shí)驗(yàn)外科雜志， 1994； 11（5）∶313.
- 5. 趙慧業(yè)，肖國衡，熊啟達(dá)，等. 胰實(shí)質(zhì)內(nèi)注射牛黃膽酸鈉引起實(shí)驗(yàn)性大鼠急性出血性胰腺炎模型及其早期損害的研究 [Ｊ]. 中國病理生理雜志， 1986； 2（2）∶112.
- 6. Lombardi B, Estes LW, Longnecker DS. Acute hemorrhagic pancreatitis (massive necrosis) with fat necrosis induced in mice by DLethionine fed with a cholinedeficient diet [Ｊ]. Am J Pathol, 1975; 79(3)∶465.
- 7. Tani S, Itoh H, Okabayashi Y, et al. New model of acute necrotizing pancreatitis induced by excessive doses of arginine in rats[Ｊ]. Dig Dis Sci, 1990; 35(3)∶367.
- 8. Vasilescu C, Herlea V, Buttenschoen K, et al. Endotoxin translocation in two models of experimental acute pancreatitis [Ｊ]. J Cell Mol Med, 2003; 7(4)∶417.
- 9. Watanabe O, Baccino FM, Steer ML, et al. Supramaximal caerulein stimulation and ultrastructure of rat pancreatic acinar cell: early morphological changes during development of experimental pancreatitis [Ｊ]. Am J Physiol, 1984; 246(4 Pt 1)∶G457.
- 10. Tani S, Otsuki M, Itoh H,et al. Histologic and biochemical alterations in experimental acute pancreatitis induced by supramaximal caerulein stimulation [Ｊ]. Int J Pancreatol, 1987; 2(5-6)∶337.