目的構(gòu)建肝癌特異性HSVTK/GCV重組腺病毒相關(guān)病毒質(zhì)粒并了解其在細(xì)胞內(nèi)的表達(dá)。方法以腺病毒相關(guān)病毒的質(zhì)粒WAV2作為載體，將TK基因插在甲胎蛋白（AFP）增強子/白蛋白啟動子(AFP增強子/ALB啟動子)的調(diào)控基因的下游，重組成pWAV2/AFPALB/HYTK質(zhì)粒載體。同時構(gòu)建質(zhì)粒載體pEGFP1/AFPALB。然后將上述兩種不同載體分別轉(zhuǎn)入AFP陽性表達(dá)的HepG2細(xì)胞株以及陰性表達(dá)的7721、SPC 和7901細(xì)胞株。結(jié)果綠色熒光蛋白只在AFP陽性表達(dá)的HepG2細(xì)胞株表達(dá)，采用PCR技術(shù)，以HSVTK的引物擴增所抽提的總DNA中，只有AFP陽性表達(dá)的HepG2細(xì)胞株擴增出了710 bp的DNA片段。結(jié)論pWAV2/AFPALB/HYTK質(zhì)粒載體在體外實驗中具有很好的靶向性。
引用本文： 李志輝,嚴(yán)律南,趙永恒,劉智敏,劉自明,李玉. 人肝癌特異性HSVTK/GCV重組腺病毒相關(guān)病毒質(zhì)粒的構(gòu)建與研究. 中國普外基礎(chǔ)與臨床雜志, 2004, 11(3): 234-235. doi: 復(fù)制
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