目的 研究幾種膽囊粘膜化學(xué)滅活劑在動物活體的滅活效果。
方法 取家兔28只，按編號法隨機分為： 生理鹽水組（對照組），95%酒精組、5%四環(huán)素組和復(fù)方苯酚組（滅活組）。行膽囊管結(jié)扎，膽囊造瘺置管并分別注入上述藥物留置15 min。2周后處死動物，對膽囊、膽管及膽囊鄰近肝臟行病理組織學(xué)檢查。
結(jié)果 所有實驗動物均存活且活動良好。肉眼觀察，對照組和5%四環(huán)素組7只鼠膽囊腔均無明顯變化； 95%酒精組中2只鼠膽囊腔閉塞，2只縮小，3只無變化； 復(fù)方苯酚組中，4只鼠膽囊腔閉塞，3只膽囊腔縮小。組織學(xué)觀察，95%酒精組及復(fù)方苯酚組中7只鼠的膽囊粘膜全部破壞（7/7），與對照組（1/7）比較差異有顯著性意義（P＜0.01）； 而5%四環(huán)素組有一定效果（5/7），但與對照組比較差異無顯著性意義（P gt;0.05）。
結(jié)論 95%酒精可以作為安全、有效的膽囊粘膜化學(xué)滅活劑應(yīng)用于臨床。
引用本文： 徐智,彭宣芙,王立新,張能維,鄧紹慶,周孝思. 幾種膽囊粘膜化學(xué)滅活劑的滅活效果評價. 中國普外基礎(chǔ)與臨床雜志, 2004, 11(2): 136-138. doi: 復(fù)制
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| 7. | Becker CD, Fache JS, Malone DE, et al. Ablation of the cystic duct and gallbladder, clinical observations [Ｊ]. Radiology, 1990; 176(3)∶687. |
| 8. | Ji ZL, Li H, Chen HR, et al. Endoscopic chemical ablation of the gallbladder [Ｊ]. Br J Surg, 1994; 81(8)∶1193. |
| 9. | Getrajdman GI, O’Toole K, Laffey KJ, et al. Cystic duct occlusion and transcatheter sclerosis of the gallbladder in the rabbit [Ｊ]. Invest Radiol, 1986; 21(5)∶400. |
- 1. Majeed AW, Reed MW, Stephenson TJ, et al. Chemical ablation of the gallbladder [Ｊ]. Br J Surg, 1997; 84(5)∶638.
- 2. Soulen MC, Sokol MC, Sullivan KL. Chemical ablation of the gallbladder: evaluation of multiple agents in vitro [Ｊ]. J Vasc Interv Radiol, 1994; 5(5)∶765.
- 3. Uchiyama N, Stridbeck H, Stenram U. Chemical sclerosis of the gallbladder. An experimental study in pigs of the effect of absolute ethanol and polidocanol on gallbladder epithelium [Ｊ]. Acta Radiol, 1989; 30(4)∶427.
- 4. Lindberg CG, Lundstedt C, Jeppsson B, et al. Sclerotherapy for ablation of the gallbladder after gallstone lithotripsy with a mechanical lithotriptor. An experimental study in pigs on the effect of absolute ethanol on edematous gallbladder wall [Ｊ]. Acta Radiol, 1991; 32(6)∶521.
- 5. Soulen MC, Sullivan KL. Chemical ablation of the gallbladder: is it feasible ? [Ｊ]. J Vasc Interv Radiol, 1995; 6(4)∶553.
- 6. Becker CD, Quenville NF, Burhenne HJ. Gallbladder ablation through radiologic intervention: an experimental alternative to cholecystectomy [Ｊ]. Radiology, 1989; 171(1)∶235.
- 7. Becker CD, Fache JS, Malone DE, et al. Ablation of the cystic duct and gallbladder, clinical observations [Ｊ]. Radiology, 1990; 176(3)∶687.
- 8. Ji ZL, Li H, Chen HR, et al. Endoscopic chemical ablation of the gallbladder [Ｊ]. Br J Surg, 1994; 81(8)∶1193.
- 9. Getrajdman GI, O’Toole K, Laffey KJ, et al. Cystic duct occlusion and transcatheter sclerosis of the gallbladder in the rabbit [Ｊ]. Invest Radiol, 1986; 21(5)∶400.