• 1.蘭州大學(xué)第二醫(yī)院普外科（蘭州730030）;;
  • 2.蘭州大學(xué)第二醫(yī)院急診外科;

【摘要】目的探討一氧化氮（NO）在大鼠梗阻性黃疸腎功能損害發(fā)生中的作用，以及褪黑素（melatonin,Mel)的保護(hù)作用。
方法64只雄性SD大鼠隨機(jī)均分為4組： 正常對(duì)照組（CN組）、假手術(shù)組（SO組）、膽總管結(jié)扎組（BLD組）和褪黑素治療組（BDL+Mel組）。應(yīng)用膽總管結(jié)扎法建立梗阻性黃疸模型，分別于手術(shù)后第4天和第8天兩個(gè)時(shí)間點(diǎn)檢測4組中血漿NO、TB、DB、ALT、AST、BUN、Cr水平以及腎組織中NO水平和iNOS活性； 光鏡下觀察腎組織病理形態(tài)改變。
結(jié)果BDL組大鼠血漿NO、TB、DB、ALT、AST、BUN、Cr水平和腎組織NO水平及iNOS活性明顯升高（P＜0.01），褪黑素治療可使血漿NO、ALT、AST、BUN及Cr水平和腎組織中NO水平及iNOS活性顯著降低，與BDL組比較差異有顯著性意義（P＜0.01）。
結(jié)論梗阻性黃疸時(shí)，腎組織iNOS活性增強(qiáng)，NO大量產(chǎn)生參與了腎功能損害的發(fā)生、發(fā)展； 褪黑素對(duì)大鼠梗阻性黃疸腎損害的保護(hù)作用至少部分是通過干擾NO通路實(shí)現(xiàn)的。

引用本文： 張小明,寇治民,康二虎,張有成. 褪黑素對(duì)大鼠梗阻性黃疸腎損害的保護(hù)作用. 中國普外基礎(chǔ)與臨床雜志, 2005, 12(1): 55-58. doi: 復(fù)制

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2. 劉祥德， 李衛(wèi)，何振平，等. NO與梗阻性黃疸時(shí)腎損害的關(guān)系 [Ｊ]. 第二軍醫(yī)大學(xué)學(xué)報(bào)， 2001； 23（7）∶795.
3. Gilad E, Wong HR, Zingarelli B, et al. Melatonin inhibits expression of the inducible isoform of nitric oxide synthase in murine macrophages: role of inhibition of NFkappaB activation [Ｊ]. FASEB J, 1998; 12(9)∶685.
4. Crespo E, Macias M, Pozo D, et al. Melatonin inhibits expression of the inducible NO synthase II in liver and lung and prevents endotoxemia in lipopolysaccharideinduced multiple organ dysfunction syndrome in rats [Ｊ]. FASEB J, 1999; 13(12)∶1537.
5. Dugo L, Serraino I, Fulia F, et al. Effect of melatonin on cellular energy depletion mediated by peroxynitrite and poly (ADPribose) synthetase activation in an acute model of inflammation [Ｊ]. J Pineal Res, 2001; 31(1)∶76.
6. Nath KA, Norby SM. Reactive oxygen species and acute renal failure [Ｊ].Am J Med， 2000; 109(8)∶ 665.
7. Kannan K, Jain SK. Oxidative stress and apoptosis [Ｊ].Pathophysiology， 2000; 7(3)∶153.
8. Jaeschke H. Reactive oxygen and mechanisms of inflammatory liver injury [Ｊ].J Gastroenterol Hepatol， 2000; 15(7)∶718.
9. Ohta Y, Kongo M, Kishikawa T. Melatonin exerts a therapeutic effect on cholestatic liver injury in rats with bile duct ligation [Ｊ]. J Pineal Res, 2003; 34(2)∶119.
10. Cruz A, Padillo FJ, Tunez I, et al. Melatonin protects against renal oxidative stress after obstructive jaundice in rats [Ｊ]. Eur J Pharmacol, 2001; 425(2)∶135.
11. Chen CY, Shiesh SC, Tsao HC, et al. Protective effect of melatonin on renal injury of rats induced by bile duct ligation [Ｊ].Dig Dis Sci, 2001; 46(4)∶927.
  1. 1. GonzalezCorrea JA, De La Cruz JP, MartinAurioles E, et al. Effects of SadenosylLmethionine on hepatic and renal oxidative stress in an experimental model of acute biliary obstruction in rats [Ｊ]. Hepatology, 1997; 26(1)∶121.
  2. 2. 劉祥德， 李衛(wèi)，何振平，等. NO與梗阻性黃疸時(shí)腎損害的關(guān)系 [Ｊ]. 第二軍醫(yī)大學(xué)學(xué)報(bào)， 2001； 23（7）∶795.
  3. 3. Gilad E, Wong HR, Zingarelli B, et al. Melatonin inhibits expression of the inducible isoform of nitric oxide synthase in murine macrophages: role of inhibition of NFkappaB activation [Ｊ]. FASEB J, 1998; 12(9)∶685.
  4. 4. Crespo E, Macias M, Pozo D, et al. Melatonin inhibits expression of the inducible NO synthase II in liver and lung and prevents endotoxemia in lipopolysaccharideinduced multiple organ dysfunction syndrome in rats [Ｊ]. FASEB J, 1999; 13(12)∶1537.
  5. 5. Dugo L, Serraino I, Fulia F, et al. Effect of melatonin on cellular energy depletion mediated by peroxynitrite and poly (ADPribose) synthetase activation in an acute model of inflammation [Ｊ]. J Pineal Res, 2001; 31(1)∶76.
  6. 6. Nath KA, Norby SM. Reactive oxygen species and acute renal failure [Ｊ].Am J Med， 2000; 109(8)∶ 665.
  7. 7. Kannan K, Jain SK. Oxidative stress and apoptosis [Ｊ].Pathophysiology， 2000; 7(3)∶153.
  8. 8. Jaeschke H. Reactive oxygen and mechanisms of inflammatory liver injury [Ｊ].J Gastroenterol Hepatol， 2000; 15(7)∶718.
  9. 9. Ohta Y, Kongo M, Kishikawa T. Melatonin exerts a therapeutic effect on cholestatic liver injury in rats with bile duct ligation [Ｊ]. J Pineal Res, 2003; 34(2)∶119.
  10. 10. Cruz A, Padillo FJ, Tunez I, et al. Melatonin protects against renal oxidative stress after obstructive jaundice in rats [Ｊ]. Eur J Pharmacol, 2001; 425(2)∶135.
  11. 11. Chen CY, Shiesh SC, Tsao HC, et al. Protective effect of melatonin on renal injury of rats induced by bile duct ligation [Ｊ].Dig Dis Sci, 2001; 46(4)∶927.