• 吉林大學(xué)第三臨床醫(yī)學(xué)院（中日聯(lián)誼醫(yī)院）普通外科（長春130033）;

【摘要】目的以人甲狀腺未分化癌細(xì)胞系TAK建立的人甲狀腺未分化癌裸鼠皮下移植模型為對象，研究裸鼠肌肉內(nèi)電轉(zhuǎn)染對移植瘤的作用。
方法將皮下移植腫瘤的裸鼠分為5組： 空白對照組、pcDNA-3質(zhì)粒肌肉電轉(zhuǎn)染組、TIMP-3質(zhì)粒肌肉注射組、TIMP-3質(zhì)粒肌肉電轉(zhuǎn)染組及TIMP-3質(zhì)粒腫瘤電轉(zhuǎn)染組。肌肉電轉(zhuǎn)染采用的參數(shù)是電場強(qiáng)度為200 V/cm，脈沖時(shí)值為20 ms，8次，1 Hz； 腫瘤內(nèi)電轉(zhuǎn)染采用的參數(shù)是電場強(qiáng)度為600 V/cm，脈沖時(shí)值為20 ms，1次，1 Hz。
結(jié)果TIMP-3質(zhì)粒肌肉內(nèi)電轉(zhuǎn)染組和TIMP-3質(zhì)粒腫瘤內(nèi)電轉(zhuǎn)染組腫瘤生長受到抑制，與另外3組相比腫瘤生長速度明顯減緩(P＜0.05)，且兩組腫瘤組織中TIMP-3蛋白量過度表達(dá)。
結(jié)論抑癌基因可通過肌肉內(nèi)DNA電轉(zhuǎn)染起到抗腫瘤效果。

引用本文： 孫輝,王金國,付言濤,欒杉,鄭澤霖. 荷瘤裸鼠肌肉內(nèi)基因電轉(zhuǎn)染對移植瘤的作用. 中國普外基礎(chǔ)與臨床雜志, 2005, 12(1): 51-54. doi: 復(fù)制

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8. Curran S, Murray GI. Matrix metalloproteinases: molecular aspects of their roles in tumour invasion and metastasis [Ｊ]. Eur J Cancer, 2000; 36(13)∶1621.
9. Baker AH, George SJ, Zaltsman AB, et al. Inhibition of invasion and induction of apoptotic cell death of cancer cell lines by overexpression of TIMP3 [Ｊ]. Br J Cancer, 1999; 79(9-10)∶1347.
10. Ahonen M, Baker AH, Kahari VM. Adenovirusmediated gene delivery of tissue inhibitor of metalloproteinases3 inhibits invasion and induces apoptosis in melanoma cells [Ｊ]. Cancer Res, 1998; 58(11)∶2310.
11. AnandApte B, Bao L, Smith R, et al. A review of tissue inhibitor of metalloproteinases3 (TIMP3) and experimental analysis of its effect on primary tumor growth [Ｊ]. Biochem Cell Biol, 1996; 74(6)∶853.
12. Bian J, Wang Y, Smith MR, et al. Suppression of in vivo tumor growth and induction of suspension cell death by tissue inhibitor of metalloproteinases (TIMP)3 [Ｊ]. Carcinogenesis , 1996; 17(9)∶1805.
13. Spurbeck WW, Ng CY, Strom TS, et al. Enforced expression of tissue inhibitor of matrix metalloproteinase3 affects functional capillary morphogenesis and inhibits tumor growth in a murine tumor model [Ｊ]. Blood, 2002; 100(9)∶3361.
14. Smith LC, Nordstrom JL. Advances in plasmid gene delivery and expression in skeletal muscle [Ｊ]. Curr Opin Mol Ther, 2000; 2(2)∶150.
15. Muramatsu T, Nakamura A, Park HM. In vivo electroporation: a powerful and convenient means of nonviral gene transfer to tissues of living animals (Review) [Ｊ]. Int J Mol Med, 1998; 1(1)∶55.
16. Somiari S, GlasspoolMalone J, Drabick JJ, et al. Theory and in vivo application of electroporative gene delivery [Ｊ]. Mol Ther, 2000; 2(3)∶178.
17. Vitadello M, Schiaffino MV, Picard A, et al. Gene transfer in regenerating muscle [Ｊ]. Hum Gene Ther, 1994; 5(1)∶11.
  1. 1. Goto T, Nishi T, Tamura T, et al. Highly efficient electrogene therapy of solid tumor by using an expression plasmid for the herpes simplex virus thymidine kinase gene [Ｊ]. Proc Natl Acad Sci USA, 2000; 97(1)∶354.
  2. 2. Folkman J. The role of angiogenesis in tumor growth [Ｊ]. Semin Cancer Biol, 1992; 3(2)∶65.
  3. 3. Bicknell R, Harris AL. Mechanisms and therapeutic implications of angiogenesis [Ｊ]. Curr Opin Oncol, 1996; 8(1)∶60.
  4. 4. Pluda JM. Tumorassociated angiogenesis: mechanisms, clinical implications, and therapeutic strategies [Ｊ]. Semin Oncol, 1997; 24(2)∶203.
  5. 5. Folkman J, Ingber D. Inhibition of angiogenesis [Ｊ]. Semin Cancer Biol, 1992; 3(2)∶89.
  6. 6. Bicknell R, Harris AL. Anticancer strategies involving the vasculature: vascular targeting and the inhibition of angiogenesis [Ｊ]. Semin Cancer Biol, 1992; 3(6)∶399.
  7. 7. Scott PA, Harris AL. Current approaches to targeting cancer using antiangiogenesis therapies [Ｊ]. Cancer Treat Rev, 1994; 20(4)∶393.
  8. 8. Curran S, Murray GI. Matrix metalloproteinases: molecular aspects of their roles in tumour invasion and metastasis [Ｊ]. Eur J Cancer, 2000; 36(13)∶1621.
  9. 9. Baker AH, George SJ, Zaltsman AB, et al. Inhibition of invasion and induction of apoptotic cell death of cancer cell lines by overexpression of TIMP3 [Ｊ]. Br J Cancer, 1999; 79(9-10)∶1347.
  10. 10. Ahonen M, Baker AH, Kahari VM. Adenovirusmediated gene delivery of tissue inhibitor of metalloproteinases3 inhibits invasion and induces apoptosis in melanoma cells [Ｊ]. Cancer Res, 1998; 58(11)∶2310.
  11. 11. AnandApte B, Bao L, Smith R, et al. A review of tissue inhibitor of metalloproteinases3 (TIMP3) and experimental analysis of its effect on primary tumor growth [Ｊ]. Biochem Cell Biol, 1996; 74(6)∶853.
  12. 12. Bian J, Wang Y, Smith MR, et al. Suppression of in vivo tumor growth and induction of suspension cell death by tissue inhibitor of metalloproteinases (TIMP)3 [Ｊ]. Carcinogenesis , 1996; 17(9)∶1805.
  13. 13. Spurbeck WW, Ng CY, Strom TS, et al. Enforced expression of tissue inhibitor of matrix metalloproteinase3 affects functional capillary morphogenesis and inhibits tumor growth in a murine tumor model [Ｊ]. Blood, 2002; 100(9)∶3361.
  14. 14. Smith LC, Nordstrom JL. Advances in plasmid gene delivery and expression in skeletal muscle [Ｊ]. Curr Opin Mol Ther, 2000; 2(2)∶150.
  15. 15. Muramatsu T, Nakamura A, Park HM. In vivo electroporation: a powerful and convenient means of nonviral gene transfer to tissues of living animals (Review) [Ｊ]. Int J Mol Med, 1998; 1(1)∶55.
  16. 16. Somiari S, GlasspoolMalone J, Drabick JJ, et al. Theory and in vivo application of electroporative gene delivery [Ｊ]. Mol Ther, 2000; 2(3)∶178.
  17. 17. Vitadello M, Schiaffino MV, Picard A, et al. Gene transfer in regenerating muscle [Ｊ]. Hum Gene Ther, 1994; 5(1)∶11.