• 中國(guó)協(xié)和醫(yī)科大學(xué)，北京協(xié)和醫(yī)院基本外科（北京100730）;

【摘要】目的 初步分析BAG3在胰腺癌抗凋亡及產(chǎn)生耐藥特性中的作用。 方法 通過免疫組化方法檢測(cè)術(shù)前化療與未化療患者手術(shù)切除的胰腺癌組織中BAG3的表達(dá)水平； 通過蛋白質(zhì)印跡方法檢測(cè)MIACaPa2、 PANC1及SW1990 3株細(xì)胞在化療藥物（5FU 50 μg/ml、 MMC 0.5 μg/ml及EADM 1.5 μg/ml）作用18 h后BAG3蛋白表達(dá)的差異。 結(jié)果 術(shù)前化療患者的胰腺癌組織中BAG3呈中強(qiáng)度陽(yáng)性表達(dá)者的比率與術(shù)前未化療患者相比有升高趨勢(shì)，但差異無(wú)統(tǒng)計(jì)學(xué)意義（P gt;0.05）。化療藥物處理后18 h，3株胰腺癌細(xì)胞中BAG3的表達(dá)水平與未經(jīng)化療藥物處理的對(duì)照組相比明顯升高（P＜0.05）。 結(jié)論 化療可使胰腺癌細(xì)胞中BAG3的表達(dá)升高，BAG3的上調(diào)可能與胰腺癌對(duì)化療產(chǎn)生耐藥相關(guān)。

引用本文： 廖泉,趙玉沛,孔令燕,吳巍巍,陳革,吳元德. 化療對(duì)胰腺癌組織及細(xì)胞中BAG-3表達(dá)的影響. 中國(guó)普外基礎(chǔ)與臨床雜志, 2005, 12(6): 591-594. doi: 復(fù)制

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  1. 1. 1 Brammer R, Buckels J, Bramhall S. Advances in non operative therapy in pancreatic cancer [Ｊ]. Int J Clin Pract， 2000; 54(6)3732 Graber HU, Friess H, Zimmermann A, et al. Bak expression and cell death occur in peritumorous tissue but not in pancreatic cancer cells [Ｊ]. J Gastrointest Surg, 1999; 3(1)743 Korc M. Role of growth factors in pancreatic cancer [Ｊ]. Surg Oncol Clin N Am, 1998; 7(1)254 Liao Q, Ozawa F, Friess H, et al. The antiapoptotic protein BAG3 is overexpressed in pancreatic cancer and induced by heat stress in pancreatic cancer cell lines [Ｊ]. FEBS Lett, 2001; 503(2-3)1515 Makinen K, Hakala T, Lipponen P, et al. Clinical contribution of bcl2, p53 and Ki67 proteins in pancreatic ductal adenocarcinoma [Ｊ]. Anticancer Res, 1998; 18(1B)6156 Bold RJ, Hess KR, Pearson AS, et al. Prognostic factors in resectable pancreatic cancer: p53 and bcl2 [Ｊ]. J Gastrointest Surg, 1999; 3(3)2637 Ariapart P, BergstedtLindqvist S, van Harmelen V, et al. Resection of pancreatic cancer normalizes the preoperative increase of tumor necrosis factor alpha gene expression [Ｊ]. Pancreatology, 2002; 2(5)4918 Trauzold A, Wermann H, Arlt A, et al. CD95 and TRAIL receptormediated activation of protein kinase C and NFkappaB contributes to apoptosis resistance in ductal pancreatic adenocarcinoma cells [Ｊ]. Oncogene, 2001; 20(31)42589 Takayama S, Xie Z, Reed JC. An evolutionarily conserved family of Hsp70/Hsc70 molecular chaperone regulators [Ｊ]. J Biol Chem, 1999; 274(2)78110 廖泉，趙玉沛，蔡力行，等. 胰腺癌細(xì)胞對(duì)化療藥物敏感性的測(cè)定 [Ｊ]. 中華實(shí)驗(yàn)外科雜志， 1998； 15（5）44411 Lee JH, Takahashi T, Yasuhara N, et al. Bis, a Bcl2binding protein that synergizes with Bcl2 in preventing cell death [Ｊ]. Oncogene, 1999; 18(46)618312 Doong H, Price J, Kim YS, et al. CAIR1/BAG3 forms an EGFregulated ternary complex with phospholipase Cgamma and Hsp70/Hsc70 [Ｊ]. Oncogene， 2000; 19(38)4385.