• 1. 第四軍醫(yī)大學西京醫(yī)院胃腸外科(西安710032) ;;
  • 2. 第四軍醫(yī)大學免疫教研室(西安710032);

目的 探討TLSFJM(JM急性T淋巴細胞白血病細胞分泌的抑制因子)作為抑制因子對小腸移植急性排斥反應的抑制效應及其機理,并與FK506的抑制效應特點和副作用進行比較分析.方法 雄性BN和LEW大鼠分別作為供、受體行小腸移植,共分5組: 小腸移植組(SBT組)、大劑量FK506 [0.5 mg/(kg·d)]組、小劑量FK506 [0.25 mg/(kg·d)]組、TLSFJM [10 U/(kg·d)]組和FK506 [0.25 mg/(kg·d)]+TLSFJM [10 U/(kg·d)]組.FK506和TLSFJM分別以肌肉或腹腔注射方式給藥.在不同時間段分別觀察各組動物體重、生存時間及肝、腎功能,組織病理學檢查排斥反應發(fā)生情況.結(jié)果 TLSFJM應用7 d,對移植大鼠肝、腎功能無損害.TLSFJM+小劑量FK506不但避免了大劑量FK506對肝功能的損害,而且顯著延長了受體的生存時間.但單獨應用TLSFJM仍有一定程度排斥反應出現(xiàn),不能明顯延長受體的生存時間.結(jié)論 TLSFJM作為一種有效的移植免疫抑制因子,聯(lián)合小劑量FK506應用能延長宿主和移植腸的生存時間,延緩排斥反應的發(fā)生,減輕排斥反應的強度,避免大劑量FK506治療帶來的并發(fā)癥.TLSFJM有望成為一種新型、高效、低毒的免疫抑制劑.

引用本文： 董光龍,李開宗,王為忠,張章,陳麗華,金伯泉. TLSFJM聯(lián)合小劑量FK506 促進大鼠小腸移植耐受. 中國普外基礎與臨床雜志, 2008, 15(1): 41-45. doi: 復制

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2. Fung JJ,Starzl TE.FK506 in solid organ transplantation[J].Transplant Proc,1994; 26(5)∶3017.
3. Guo WH,Tian L,Dallmann MJ,et al.Comparative study of allograft survival of heterotopic and orthotopic small bowel transplantation in rat[J].Transplantation,2003; 75(11)∶1895.
4. Higuchi K,Kimura O,Furukawa T,et al.FK506 induces the atrophy of enteric ganglia in small bowel transplantation,which can be prevented by the neuropeptide bombesin[J].Transplant Proc,2006; 38(6)∶1823.
5. 董光龍,李開宗,王為忠.雙Cuff雙造口大鼠異位小腸移植模型的建立[J].第四軍醫(yī)大學學報,2003; 24(2)∶189.
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7. 魏曉平,李立.他克莫司與環(huán)孢素A在器官移植中的應用比較[J].中國普外基礎與臨床雜志,2003; 10(4)∶415.
8. Jiang H,Kobayashi M.Differences between cyclosporin A and tacrolimus in organ transplantation[J].Transplant Proc,1999; 31(5)∶1978.
9. Miyauchi T,Ishikawa M,Tashiro S,et al.Effect of donor-specific splenocytes via portal vein and FK506 in rat small bowel transplantation[J].Transplantation,1998; 65(1)∶27.
10. 金伯泉.一株人急性淋巴細胞白血病細胞所分泌抑制因子的免疫調(diào)節(jié)作用[J].中華血液學雜志,1990; 11(5)∶247.
11. 申立中,金伯泉,夏海濱,等.TLSFJM對DNFB誘導的小鼠接觸性皮炎和同種異體皮片移植排斥反應的抑制作用[J].細胞與分子免疫學雜志,1996; 12(4)∶27.
12. 韓衛(wèi)寧,李德敏,蘇麗,等.TLSFJM對同種異體抗原誘導的小鼠殺傷性T細胞分化及效應功能的影響[J].細胞與分子免疫學雜志,1999; 15(2)∶118.
13. Guo WH,Tian L,Chan KL,et al.Role of CD4+ and CD8+ T cells in early and late acute rejection of small bowel allograft[J].J Pediatr Surg,2001; 36(2)∶352.
14. Ma H,Wang J,Wang J,et al.Features of chronic allograft rejection on rat small intestine transplantation[J].Pediatr Transplant,2007; 11(2)∶165.
15. Song J,Ito T,Matsuda C,et al.Regulation of donor T cells in the tolerant rats to graft-versus-host disease by FTY720 following small bowel transplantation[J].Transplant Proc,2006; 38(10)∶3181.
16. Gasser M,Otto C,Timmermann W,et al.Putative regulatory function of alloreactive Th2 cells in tolerant recipients after small bowel transplantation[J].Transplant Proc,2001; 33(1-2)∶161.
17. 智星,嚴律南,楊培,等.CTLA4-Ig抗大鼠肝移植排斥反應及誘導免疫耐受的實驗研究[J].中國普外基礎與臨床雜志,2005; 12(6)∶600.
18. 董光龍,李開宗,王為忠.TLSFJM對小腸移植大鼠外周血及移植腸浸潤淋巴細胞的影響[J].免疫學雜志,2003; 19(1)∶36.
  1. 1. <>[1]Tzakis AG,Nery JR,Thompson J,et al.New immunosuppressive regimens in clinical intestinal transplantation[J].Transplant Proc,1997; 29(1-2)∶683.
  2. 2. Fung JJ,Starzl TE.FK506 in solid organ transplantation[J].Transplant Proc,1994; 26(5)∶3017.
  3. 3. Guo WH,Tian L,Dallmann MJ,et al.Comparative study of allograft survival of heterotopic and orthotopic small bowel transplantation in rat[J].Transplantation,2003; 75(11)∶1895.
  4. 4. Higuchi K,Kimura O,Furukawa T,et al.FK506 induces the atrophy of enteric ganglia in small bowel transplantation,which can be prevented by the neuropeptide bombesin[J].Transplant Proc,2006; 38(6)∶1823.
  5. 5. 董光龍,李開宗,王為忠.雙Cuff雙造口大鼠異位小腸移植模型的建立[J].第四軍醫(yī)大學學報,2003; 24(2)∶189.
  6. 6. Grant D.Current results of intestinal transplantation.The International Intestinal Transplant Registry[J].Lancet,1996; 347(9018)∶1801.
  7. 7. 魏曉平,李立.他克莫司與環(huán)孢素A在器官移植中的應用比較[J].中國普外基礎與臨床雜志,2003; 10(4)∶415.
  8. 8. Jiang H,Kobayashi M.Differences between cyclosporin A and tacrolimus in organ transplantation[J].Transplant Proc,1999; 31(5)∶1978.
  9. 9. Miyauchi T,Ishikawa M,Tashiro S,et al.Effect of donor-specific splenocytes via portal vein and FK506 in rat small bowel transplantation[J].Transplantation,1998; 65(1)∶27.
  10. 10. 金伯泉.一株人急性淋巴細胞白血病細胞所分泌抑制因子的免疫調(diào)節(jié)作用[J].中華血液學雜志,1990; 11(5)∶247.
  11. 11. 申立中,金伯泉,夏海濱,等.TLSFJM對DNFB誘導的小鼠接觸性皮炎和同種異體皮片移植排斥反應的抑制作用[J].細胞與分子免疫學雜志,1996; 12(4)∶27.
  12. 12. 韓衛(wèi)寧,李德敏,蘇麗,等.TLSFJM對同種異體抗原誘導的小鼠殺傷性T細胞分化及效應功能的影響[J].細胞與分子免疫學雜志,1999; 15(2)∶118.
  13. 13. Guo WH,Tian L,Chan KL,et al.Role of CD4+ and CD8+ T cells in early and late acute rejection of small bowel allograft[J].J Pediatr Surg,2001; 36(2)∶352.
  14. 14. Ma H,Wang J,Wang J,et al.Features of chronic allograft rejection on rat small intestine transplantation[J].Pediatr Transplant,2007; 11(2)∶165.
  15. 15. Song J,Ito T,Matsuda C,et al.Regulation of donor T cells in the tolerant rats to graft-versus-host disease by FTY720 following small bowel transplantation[J].Transplant Proc,2006; 38(10)∶3181.
  16. 16. Gasser M,Otto C,Timmermann W,et al.Putative regulatory function of alloreactive Th2 cells in tolerant recipients after small bowel transplantation[J].Transplant Proc,2001; 33(1-2)∶161.
  17. 17. 智星,嚴律南,楊培,等.CTLA4-Ig抗大鼠肝移植排斥反應及誘導免疫耐受的實驗研究[J].中國普外基礎與臨床雜志,2005; 12(6)∶600.
  18. 18. 董光龍,李開宗,王為忠.TLSFJM對小腸移植大鼠外周血及移植腸浸潤淋巴細胞的影響[J].免疫學雜志,2003; 19(1)∶36.