• 1. 海南醫(yī)學(xué)院附屬醫(yī)院(海口570102) ;;
  • 2. 重慶醫(yī)科大學(xué)附屬第一醫(yī)院普外科(重慶400016) ;;
  • 3. 重慶醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院病理生理教研室(重慶400016) ;;
  • 4. 四川省廣安市人民醫(yī)院普外科(廣安638000);

目的　研究金雀異黃素( genistein , Gen) 與52氟尿嘧啶(52fluorouracil ,52FU) 對人胃癌細(xì)胞SGC-7901 的抑制作用。方法　采用MTT 法檢測Gen 和5-FU 對SGC-7901 細(xì)胞的抑制作用,流式細(xì)胞術(shù)檢測細(xì)胞周期分布,并在透射電鏡下觀察細(xì)胞超微結(jié)構(gòu)改變。結(jié)果　Gen 和5-FU 單用或聯(lián)合應(yīng)用對胃癌SGC27901 細(xì)胞的生長均有顯著的增殖抑制作用,且呈劑量和時間依賴性。兩藥聯(lián)合應(yīng)用時,當(dāng)藥物效應(yīng)在0. 2～0. 8 時,具有協(xié)同或相加作用(CI ≤1) ; 18. 8μmol/ L 的Gen 作用后,62. 97 %的SGC-7901 細(xì)胞阻滯在G2 / M 期; 8. 84μmol/ L 的52FU作用后,63. 76 %的SGC-7901 細(xì)胞阻滯在S 期; 3. 06 μmol/ L 的Gen 與7. 96 μmol/ L 的5-FU 聯(lián)合應(yīng)用后,67. 46 %的SGC27901 細(xì)胞阻滯在G0 / G1 期。Gen 和52FU 均可誘導(dǎo)SGC-7901 細(xì)胞凋亡。結(jié)論　Gen 與5-FU 通過阻滯細(xì)胞周期和誘導(dǎo)細(xì)胞凋亡而抑制胃癌細(xì)胞的生長,對胃癌細(xì)胞的增殖抑制具有協(xié)同作用。

引用本文： 王正文,朱明才,湯為學(xué),龔軍,范維珂,魏錦來,崔海寧. 金雀異黃素與5-氟尿嘧啶對SGC-7901 細(xì)胞的抑制作用. 中國普外基礎(chǔ)與臨床雜志, 2008, 15(1): 17-22. doi: 復(fù)制

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2. 龔軍,王正文,朱明才,等.金雀異黃素和5-FU對人結(jié)腸癌細(xì)胞株(SW480)的相互作用的體外觀察[J].中國現(xiàn)代醫(yī)學(xué)雜志,2006; 16(6)∶873.
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6. Yu Z,Li W,Liu F.Inhibition of proliferation and induction of apoptosis by genistein in colon cancer HT-29 cells[J].Cancer Lett,2004; 215(2)∶159.
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8. Chou TC,Talalay P.Quantitative analysis of dose-effect relationships:the combined effects of multiple drugs or enzyme inhibitors[J].Adv Enzyme Regul,1984; 22∶27.
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11. Davis JN,Singh B,Bhuiyan M,et al.Genistein-induced upregulation of p21WAF1,downregulation of cyclin B,and induction of apoptosis in prostate cancer cells[J].Nutr Cancer,1998; 32(3)∶123.
12. Yan C,Han R.Genistein suppresses adhesion-induced protein tyrosine phosphorylation and invasion of B16-BL6 melanoma cells[J].Cancer Lett,1998; 129(1)∶117.
13. Arai N,Strom A,Rafter JJ,et al.Estrogen receptor beta mRNA in colon cancer cells:growth effects of estrogen and genistein[J].Biochem Biophys Res Commun,2000; 270(2)∶425.
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19. Hwang JT,Ha J,Park OJ.Combination of 5-fluorouracil and genistein induces apoptosis synergistically in chemo-resistant cancer cells through the modulation of AMPK and COX-2 signaling pathways[J].Biochem Biophys Res Commun,2005; 332(2)∶433.
20. Banerjee S,Zhang Y,Wang Z,et al.In vitro and in vivo molecular evidence of genistein action in augmenting the efficacy of cisplatin in pancreatic cancer[J].Int J Cancer,2007; 120(4)∶906.
21. Siwinska A,Opolski A,Chrobak A,et al.Potentiation of the antiproliferative effect in vitro of doxorubicin,cisplatin and genistein by new analogues of vitamin D[J].Anticancer Res,2001; 21(3B)∶1925.
22. McDonnell CO,Holden G,Sheridan ME,et al.Improvement in efficacy of chemoradiotherapy by addition of an antiangiogenic agent in a murine tumor model[J].J Surg Res,2004; 116(1)∶19.
23. Tamura S,Bito T,Ichihashi M,et al.Genistein enhances the cisplatin-induced inhibition of cell growth and apoptosis in human malignant melanoma cells[J].Pigment Cell Res,2003; 16(5)∶470.
24. Evan GI,Vousden KH.Proliferation,cell cycle and apoptosis in cancer[J].Nature,2001; 411(6835)∶342.
25. 葉正寶,馬韜.胃腸道癌瘤的化學(xué)治療[A].見:朱正綱主編.胃腸道腫瘤外科綜合治療新技術(shù)[M].北京:人民軍醫(yī)出版社,2002∶154～180.
  1. 1. <><> [1]Barnes S.Effect of genistein on in vitro and in vivo models of cancer[J].J Nutr,1995; 125(3 Suppl)∶777S.
  2. 2. 龔軍,王正文,朱明才,等.金雀異黃素和5-FU對人結(jié)腸癌細(xì)胞株(SW480)的相互作用的體外觀察[J].中國現(xiàn)代醫(yī)學(xué)雜志,2006; 16(6)∶873.
  3. 3. 龔軍,王正文,朱明才,等.金雀異黃素和5-FU對人結(jié)腸癌細(xì)胞株(Colo320)的相互作用的體外觀察[J].中國醫(yī)師雜志,2006; 8(12)∶1601.
  4. 4. 張艷,田慶偉,李亞義.金雀異黃素對人乳癌細(xì)胞MCF-7增殖和凋亡的影響[J].天津醫(yī)科大學(xué)學(xué)報,2005; 11(1)∶42.
  5. 5. Shon YH,Park SD,Nam KS.Effective chemopreventive activity of genistein against human breast cancer cells[J].J Biochem Mol Biol,2006; 39(4)∶448.
  6. 6. Yu Z,Li W,Liu F.Inhibition of proliferation and induction of apoptosis by genistein in colon cancer HT-29 cells[J].Cancer Lett,2004; 215(2)∶159.
  7. 7. Bektic J,Guggenberger R,Eder IE,et al.Molecular effects of the isoflavonoid genistein in prostate cancer[J].Clin Prostate Cancer,2005; 4(2)∶124.
  8. 8. Chou TC,Talalay P.Quantitative analysis of dose-effect relationships:the combined effects of multiple drugs or enzyme inhibitors[J].Adv Enzyme Regul,1984; 22∶27.
  9. 9. 韓銳主編.抗癌藥物研究與實(shí)驗(yàn)技術(shù)[M].北京:北京醫(yī)科大學(xué)中國協(xié)和醫(yī)科大學(xué)聯(lián)合出版社,1997∶399～400.
  10. 10. Shao ZM,Wu J,Shen ZZ,et al.Genistein exerts multiple suppressive effects on human breast carcinoma cells[J].Cancer Res,1998; 58(21)∶4851.
  11. 11. Davis JN,Singh B,Bhuiyan M,et al.Genistein-induced upregulation of p21WAF1,downregulation of cyclin B,and induction of apoptosis in prostate cancer cells[J].Nutr Cancer,1998; 32(3)∶123.
  12. 12. Yan C,Han R.Genistein suppresses adhesion-induced protein tyrosine phosphorylation and invasion of B16-BL6 melanoma cells[J].Cancer Lett,1998; 129(1)∶117.
  13. 13. Arai N,Strom A,Rafter JJ,et al.Estrogen receptor beta mRNA in colon cancer cells:growth effects of estrogen and genistein[J].Biochem Biophys Res Commun,2000; 270(2)∶425.
  14. 14. Ravindranath MH,Muthugounder S,Presser N,et al.Anticancer therapeutic potential of soy isoflavone,genistein[J].Adv Exp Med Biol,2004; 546∶121.
  15. 15. Sarkar FH,Adsule S,Padhye S,et al.The role of genistein and synthetic derivatives of isoflavone in cancer prevention and therapy[J].Mini Rev Med Chem,2006; 6(4)∶401.
  16. 16. 金懋林.進(jìn)展期胃癌全身化學(xué)治療新進(jìn)展[J].外科理論與實(shí)踐,2003; 8(1)∶18.
  17. 17. 劉福坤,姚學(xué)權(quán).胃癌的新輔助化療[J].中國普外基礎(chǔ)與臨床雜志,2006; 13(1)∶17.
  18. 18. 黃斌,陳志新.胃癌淋巴靶向化療的研究進(jìn)展[J].中國普外基礎(chǔ)與臨床雜志,2006; 13(5)∶ 617.
  19. 19. Hwang JT,Ha J,Park OJ.Combination of 5-fluorouracil and genistein induces apoptosis synergistically in chemo-resistant cancer cells through the modulation of AMPK and COX-2 signaling pathways[J].Biochem Biophys Res Commun,2005; 332(2)∶433.
  20. 20. Banerjee S,Zhang Y,Wang Z,et al.In vitro and in vivo molecular evidence of genistein action in augmenting the efficacy of cisplatin in pancreatic cancer[J].Int J Cancer,2007; 120(4)∶906.
  21. 21. Siwinska A,Opolski A,Chrobak A,et al.Potentiation of the antiproliferative effect in vitro of doxorubicin,cisplatin and genistein by new analogues of vitamin D[J].Anticancer Res,2001; 21(3B)∶1925.
  22. 22. McDonnell CO,Holden G,Sheridan ME,et al.Improvement in efficacy of chemoradiotherapy by addition of an antiangiogenic agent in a murine tumor model[J].J Surg Res,2004; 116(1)∶19.
  23. 23. Tamura S,Bito T,Ichihashi M,et al.Genistein enhances the cisplatin-induced inhibition of cell growth and apoptosis in human malignant melanoma cells[J].Pigment Cell Res,2003; 16(5)∶470.
  24. 24. Evan GI,Vousden KH.Proliferation,cell cycle and apoptosis in cancer[J].Nature,2001; 411(6835)∶342.
  25. 25. 葉正寶,馬韜.胃腸道癌瘤的化學(xué)治療[A].見:朱正綱主編.胃腸道腫瘤外科綜合治療新技術(shù)[M].北京:人民軍醫(yī)出版社,2002∶154～180.