• 1.綿陽(yáng)市中心醫(yī)院肝膽外科(四川綿陽(yáng)　621000);;
  • ２.綿陽(yáng)市中心醫(yī)院檢驗(yàn)科（四川綿陽(yáng)　621000）;

目的 探討HBsAg陽(yáng)性肝細(xì)胞肝癌（HCC）患者乙型肝炎病毒（HBV）DNA基因型與其患HCC之間的關(guān)系。方法 運(yùn)用PCR條帶分析與基因測(cè)序相結(jié)合的方法對(duì)我院500例HBsAg陽(yáng)性患者（其中HCC患者150例）的HBV DNA進(jìn)行分型，并對(duì)分型結(jié)果進(jìn)行分析。結(jié)果 HBV DNA B型和C型基因型是HBsAg陽(yáng)性HCC患者和非HCC患者的共同優(yōu)勢(shì)基因型，但HCC患者中C型基因型所占比例為65.33%（98/150），明顯高于非HCC患者的25.14%（88/350），而B(niǎo)型則相反，分別為28.67%（43/150）與68.86%（241/350），χ2=75.45，P lt;0.05。HCC患者中，HBV的B型與C型基因型分布在不同性別及不同年齡段之間的差異無(wú)統(tǒng)計(jì)學(xué)意義（P gt;0.05）。結(jié)論 HBV DNA C型基因型在HCC患者中多見(jiàn)，可能與HCC的發(fā)生有關(guān)。

引用本文： 胡朝輝,羅華,楊培,鄧文斌. 乙型肝炎病毒基因型與肝細(xì)胞肝癌關(guān)系的研究△. 中國(guó)普外基礎(chǔ)與臨床雜志, 2010, 17(12): 1261-1264. doi: 復(fù)制

1. Thakur V, Guptan RC, Kazim SN, et al. Profile, spectrum and significance of HBV genotypes in chronic liver disease patients in the Indian subcontinent [J]. J Gastroenterol Hepatol, 2002; 17(2): 165-170.
2. 胡朝輝， 羅華， 鄧文斌, 等. 四川地區(qū)乙型肝炎患者HBV基因型分布特點(diǎn)研究 [J]. 西部醫(yī)學(xué), 2009; 21（12）: 2114-2115.
3. 中國(guó)抗癌協(xié)會(huì)肝癌專業(yè)委員會(huì). 原發(fā)性肝癌診斷標(biāo)準(zhǔn) [J]. 中華肝臟病雜志, 2000; 8(3): 135.
4. Naito H, Hayashi S, Abe K. Rapid and specific Rapid and specific genotyping system for hepatitis B virus corresponding to six major genotypes by PCR using type-specific primers [J]. J Clin Microbiol, 2001; 39(1): 362-364.
5. 黃志強(qiáng)主譯. 希夫肝臟病學(xué) [M]. 北京: 化學(xué)工業(yè)出版社, 2006: 689, 1230.
6. 吳孟超. 肝癌外科治療的近期進(jìn)展 [J]. 中國(guó)普外基礎(chǔ)與臨床雜志, 2006; 13（2）: 125-128.
7. 肖開(kāi)銀， 彭民浩. 原發(fā)性肝癌流行病學(xué)研究進(jìn)展 [J]. 中國(guó)普外基礎(chǔ)與臨床雜志, 2000; 7（4）: 272-274.
8. Lin CL, Chen JD, Liu CJ, et al. Clinicopathological differences between hepatitis B viral genotype B- and C-related resectable hepatocellular carcinoma [J]. J Viral Hepat, 2007; 14(1): 64-69.
9. Gao ZY, Li T, Wang J, et al. Mutations in preS genes of genotype C hepatitis B virus in patients with chronic hepatitis B and hepatocellular carcinoma [J]. J Gastroenterol, 2007; 42(9): 761-768.
10. Wang Z, Tanaka Y, Huang Y, et al. Clinical and virological characteristics of hepatitis B virus subgenotypes Ba, C1, and C2 in China [J]. J Clin Microbiol, 2007; 45(5): 1491-1496.
11. 林旭， 徐曉， 鄭大利, 等. 肝癌患者乙型肝炎病毒全基因組結(jié)構(gòu)分析 [J]. 中華腫瘤雜志, 2004; 26（4）： 213-216.
12. Livingston SE, Simonetti JP, McMahon BJ, et al. Hepatitis B virus genotypes in Alaska Native people with hepatocellular carcinoma: preponderance of genotype F [J]. J Infect Dis, 2007; 195(1): 5-11.
13. Elkady A, Tanaka Y, Kurbanov F, et al. Virological and clinical implication of core promoter C1752/V1753 and T1764/G1766 mutations in hepatitis B virus genotype D infection in Mongolia [J]. J Gastroenterol Hepatol, 2008; 23(3): 474-481.
14. Shrestha SM, Shrestha S, Shrestha A, et al. High prevalence of hepatitis B virus infection and inferior vena cava obstruction among patients with liver cirrhosis or hepatocellular carcinoma in Nepal [J]. Journal J Gastroenterol Hepatol, 2007; 22(11): 1921-1928.
15. Kew MC, Kramvis A, Yu MC, et al. Increased hepatocarcinogenic potential of hepatitis B virus genotype A in Bantu-speaking sub-saharan Africans [J]. J Med Virol, 2005; 75(4): 513-521.
16. Tangkijvanich P, Mahachai V, Komolmit P, et al. Hepatitis B virus genotypes and hepatocellular carcinoma in Thailand [J]. World J Gastroenterol, 2005; 11(15): 2238-2243.
17. Yu MW, Yeh SH, Chen PJ, et al. Hepatitis B virus genotype and DNA level and hepatocellular carcinoma: a prospective study in men [J]. J Natl Cancer Inst, 2005; 97(4): 265-272.
18. Ito K, Tanaka Y, Kato M, et al. Comparison of complete sequences of hepatitis B virus genotype C between inactive carriers and hepatocellular carcinoma patients before and after seroconversion [J]. J Gastroenterol, 2007; 42(10): 837-844.
19. Choi MS, Kim DY, Lee DH, et al. Clinical significance of pre-S mutations in patients with genotype C hepatitis B virus infection [J]. J Viral Hepat, 2007; 14(3): 161-168.
20. Lin CL, Kao JH. Hepatitis B viral factors and clinical outcomes of chronic hepatitis B [J]. J Biomed Sci, 2008; 15(2): 137-145.
21. Tong MJ, Blatt LM, Kao JH, et al. Basal core promoter T1762/A1764 and precore A1896 gene mutations in hepatitis B surface antigen-positive hepatocellular carcinoma: a comparison with chronic carriers [J]. Liver Int, 2007; 27(10): 1356-1363.
22. Shinkai N, Tanaka Y, Ito K, et al. Influence of hepatitis B virus X and core promoter mutations on hepatocellular carcinoma among patients infected with subgenotype C2 [J]. J Clin Microbiol, 2007; 45(10): 3191-3197.
23. Truong BX, Yano Y, Seo Y, et al. Variations in the core promoter/pre-core region in HBV genotype C in Japanese and Northern Vietnamese patients [J]. J Med Virol, 2007; 79(9): 1293-1304.
24. Ito K, Tanaka Y, Orito E, et al. T1653 mutation in the box alpha increases the risk of hepatocellular carcinoma in patients with chronic hepatitis B virus genotype C infection [J]. Clin Infect Dis, 2006; 42(1): 1-7.
  1. 1. Thakur V, Guptan RC, Kazim SN, et al. Profile, spectrum and significance of HBV genotypes in chronic liver disease patients in the Indian subcontinent [J]. J Gastroenterol Hepatol, 2002; 17(2): 165-170.
  2. 2. 胡朝輝， 羅華， 鄧文斌, 等. 四川地區(qū)乙型肝炎患者HBV基因型分布特點(diǎn)研究 [J]. 西部醫(yī)學(xué), 2009; 21（12）: 2114-2115.
  3. 3. 中國(guó)抗癌協(xié)會(huì)肝癌專業(yè)委員會(huì). 原發(fā)性肝癌診斷標(biāo)準(zhǔn) [J]. 中華肝臟病雜志, 2000; 8(3): 135.
  4. 4. Naito H, Hayashi S, Abe K. Rapid and specific Rapid and specific genotyping system for hepatitis B virus corresponding to six major genotypes by PCR using type-specific primers [J]. J Clin Microbiol, 2001; 39(1): 362-364.
  5. 5. 黃志強(qiáng)主譯. 希夫肝臟病學(xué) [M]. 北京: 化學(xué)工業(yè)出版社, 2006: 689, 1230.
  6. 6. 吳孟超. 肝癌外科治療的近期進(jìn)展 [J]. 中國(guó)普外基礎(chǔ)與臨床雜志, 2006; 13（2）: 125-128.
  7. 7. 肖開(kāi)銀， 彭民浩. 原發(fā)性肝癌流行病學(xué)研究進(jìn)展 [J]. 中國(guó)普外基礎(chǔ)與臨床雜志, 2000; 7（4）: 272-274.
  8. 8. Lin CL, Chen JD, Liu CJ, et al. Clinicopathological differences between hepatitis B viral genotype B- and C-related resectable hepatocellular carcinoma [J]. J Viral Hepat, 2007; 14(1): 64-69.
  9. 9. Gao ZY, Li T, Wang J, et al. Mutations in preS genes of genotype C hepatitis B virus in patients with chronic hepatitis B and hepatocellular carcinoma [J]. J Gastroenterol, 2007; 42(9): 761-768.
  10. 10. Wang Z, Tanaka Y, Huang Y, et al. Clinical and virological characteristics of hepatitis B virus subgenotypes Ba, C1, and C2 in China [J]. J Clin Microbiol, 2007; 45(5): 1491-1496.
  11. 11. 林旭， 徐曉， 鄭大利, 等. 肝癌患者乙型肝炎病毒全基因組結(jié)構(gòu)分析 [J]. 中華腫瘤雜志, 2004; 26（4）： 213-216.
  12. 12. Livingston SE, Simonetti JP, McMahon BJ, et al. Hepatitis B virus genotypes in Alaska Native people with hepatocellular carcinoma: preponderance of genotype F [J]. J Infect Dis, 2007; 195(1): 5-11.
  13. 13. Elkady A, Tanaka Y, Kurbanov F, et al. Virological and clinical implication of core promoter C1752/V1753 and T1764/G1766 mutations in hepatitis B virus genotype D infection in Mongolia [J]. J Gastroenterol Hepatol, 2008; 23(3): 474-481.
  14. 14. Shrestha SM, Shrestha S, Shrestha A, et al. High prevalence of hepatitis B virus infection and inferior vena cava obstruction among patients with liver cirrhosis or hepatocellular carcinoma in Nepal [J]. Journal J Gastroenterol Hepatol, 2007; 22(11): 1921-1928.
  15. 15. Kew MC, Kramvis A, Yu MC, et al. Increased hepatocarcinogenic potential of hepatitis B virus genotype A in Bantu-speaking sub-saharan Africans [J]. J Med Virol, 2005; 75(4): 513-521.
  16. 16. Tangkijvanich P, Mahachai V, Komolmit P, et al. Hepatitis B virus genotypes and hepatocellular carcinoma in Thailand [J]. World J Gastroenterol, 2005; 11(15): 2238-2243.
  17. 17. Yu MW, Yeh SH, Chen PJ, et al. Hepatitis B virus genotype and DNA level and hepatocellular carcinoma: a prospective study in men [J]. J Natl Cancer Inst, 2005; 97(4): 265-272.
  18. 18. Ito K, Tanaka Y, Kato M, et al. Comparison of complete sequences of hepatitis B virus genotype C between inactive carriers and hepatocellular carcinoma patients before and after seroconversion [J]. J Gastroenterol, 2007; 42(10): 837-844.
  19. 19. Choi MS, Kim DY, Lee DH, et al. Clinical significance of pre-S mutations in patients with genotype C hepatitis B virus infection [J]. J Viral Hepat, 2007; 14(3): 161-168.
  20. 20. Lin CL, Kao JH. Hepatitis B viral factors and clinical outcomes of chronic hepatitis B [J]. J Biomed Sci, 2008; 15(2): 137-145.
  21. 21. Tong MJ, Blatt LM, Kao JH, et al. Basal core promoter T1762/A1764 and precore A1896 gene mutations in hepatitis B surface antigen-positive hepatocellular carcinoma: a comparison with chronic carriers [J]. Liver Int, 2007; 27(10): 1356-1363.
  22. 22. Shinkai N, Tanaka Y, Ito K, et al. Influence of hepatitis B virus X and core promoter mutations on hepatocellular carcinoma among patients infected with subgenotype C2 [J]. J Clin Microbiol, 2007; 45(10): 3191-3197.
  23. 23. Truong BX, Yano Y, Seo Y, et al. Variations in the core promoter/pre-core region in HBV genotype C in Japanese and Northern Vietnamese patients [J]. J Med Virol, 2007; 79(9): 1293-1304.
  24. 24. Ito K, Tanaka Y, Orito E, et al. T1653 mutation in the box alpha increases the risk of hepatocellular carcinoma in patients with chronic hepatitis B virus genotype C infection [J]. Clin Infect Dis, 2006; 42(1): 1-7.