• 四川大學(xué)華西醫(yī)院 1呼吸內(nèi)科;2實(shí)驗(yàn)醫(yī)學(xué)科，四川成都 610041;

研究白介素6(IL-6)在過(guò)氧化氫誘導(dǎo)的肺泡上皮細(xì)胞凋亡中的作用及其機(jī)制。方法：以人Ⅱ型肺泡上皮細(xì)胞A549細(xì)胞株為實(shí)驗(yàn)對(duì)象，MTT法檢測(cè)IL-6對(duì)A549細(xì)胞增殖活性的影響，TUNEL檢測(cè)法檢測(cè)IL-6對(duì)A549細(xì)胞凋亡率的影響，Western blot觀察caspase-3和caspase-9蛋白量的變化。結(jié)果：IL-6能夠增加A549細(xì)胞的增殖活性，減少其凋亡。與模型組相比，IL-6干預(yù)組的caspase-3和caspase-9的蛋白表達(dá)量明顯降低,有統(tǒng)計(jì)學(xué)意義。結(jié)論：IL-6能夠抑制過(guò)氧化氫誘導(dǎo)的肺泡上皮細(xì)胞凋亡，并且與caspase-3和caspase-9蛋白的表達(dá)量下降有關(guān)。提示IL-6對(duì)肺泡上皮細(xì)胞的保護(hù)作用可能是通過(guò)抑制細(xì)胞凋亡相關(guān)蛋白來(lái)實(shí)現(xiàn)的。

引用本文： 楊冬梅,丁宇,應(yīng)斌武,蔣飛,美朗曲措,范紅. 白介素6對(duì)過(guò)氧化氫誘導(dǎo)的肺泡上皮細(xì)胞凋亡的影響. 華西醫(yī)學(xué), 2008, 23(1): 98-100. doi: 復(fù)制

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