• 四川大學(xué)華西醫(yī)院 1普外科;2生物治療國(guó)家重點(diǎn)實(shí)驗(yàn)室實(shí)驗(yàn)?zāi)[瘤研究室，四川成都 610041;

目的：探討Na+，K+-ATP1b1（ATP1b1）對(duì)肝癌細(xì)胞生長(zhǎng)及侵襲能力的影響。方法：將ATP1b1表達(dá)質(zhì)粒ATP1b1-CMV-FLAG轉(zhuǎn)染肝癌細(xì)胞SMMC-7721，以空質(zhì)粒轉(zhuǎn)染作對(duì)照，通過RT-PCR測(cè)定ATP1b1的mRNA表達(dá)，在光鏡下觀察細(xì)胞形態(tài)學(xué)變化，MTT法檢測(cè)細(xì)胞生長(zhǎng)增殖，Transwell細(xì)胞侵襲實(shí)驗(yàn)分析肝癌細(xì)胞的侵襲能力。結(jié)果：ATP1b1-CMV-FLAG轉(zhuǎn)染后，SMMC-7721的ATP1b1 mRNA表達(dá)水平明顯增高（P＜0.05）,轉(zhuǎn)染ATP1b1-CMV-FLAG組、pFLAG-CMV-1組和脂質(zhì)體組的ATP1b1 mRNA表達(dá)水平分別為1.159、0.182和0.093;轉(zhuǎn)染ATP1b1-CMV-FLAG的SMMC-7721細(xì)胞呈變性改變，其生長(zhǎng)受到抑制，轉(zhuǎn)染相同DNA濃度的ATP1b1-CMV-FLAG組與pFLAG-CMV-1組對(duì)細(xì)胞生長(zhǎng)的抑制效應(yīng)有顯著性差異（P＜0.05）；Transwell細(xì)胞侵襲實(shí)驗(yàn)顯示轉(zhuǎn)染ATP1b1-CMV-FLAG組的SMMC-7721細(xì)胞的體外侵襲力明顯受抑制（P＜0.01）。結(jié)論：實(shí)驗(yàn)結(jié)果說明了促進(jìn)ATP1b1表達(dá)能抑制肝癌細(xì)胞的生長(zhǎng)及侵襲行為。

引用本文： 劉戰(zhàn)培,熊竹娟,張潔,林蘋,王修杰,楊洪亮,任婧婧. Na+,K+-ATP1b1對(duì)肝癌細(xì)胞生長(zhǎng)侵襲能力影響的研究. 華西醫(yī)學(xué), 2008, 23(1): 94-96. doi: 復(fù)制

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  1. 1. secondary structure[Ｊ].J Mol Biol,1999,288:911-940.
  2. 2. chen Q,Watson JT,Marengo SR,et al.Gene expression in the LNCaP humanprostate cancer progression model: progression associated expression in vitrocorresponds to expression changes associated with prostate cancer progressionin vivo[Ｊ].Cancer Lett,2006,244(2):274-２88..
  3. 3. Le Page C,Ouellet V,Madore J,et al.Gene expression profiling of primary cultures of ovarian epithelial cells identifies novel molecular classifiers of ovarian cancer[Ｊ].Br J Cancer,2006,94(3):436-４45..
  4. 4. Lingrel JB,Kuntzweiler T.Na+,K(+)-ATPase.J Biol Chem,1994,269(31):19 659-19 662..
  5. 5. Shoshani L,Contreras RG,Roldan ML,et al.The polarized expression of Na+,K+-ATPase in epithelia depends on the association between beta-subunits located in neighboring cells[Ｊ].Mol Biol Cell,2005,16(3):1 071-81..
  6. 6. Mathews DH,Sabina J,Zuker M,et al.Expanded sequence dependence of thermodynamic parameters improves prediction of RNA.
  7. 7. Chow DC,Forte JG.Functional significance of the β-subunit for heterodimeric P-type ATPases[Ｊ].J Exp Biol,1995,198:1-17..
  8. 8. Fambrough DM,Lemas MV,Hamrick M,et al.Analysis of subunit assembly of the Na-K-ATPase[Ｊ].Am J Physiol,1994,266(3 Pt 1):C579-C589．.
  9. 9. Beguin P,Hasler U,Staub O,et al.Endoplasmic reticulum quality control of oligomeric membrane proteins:topogenic determinants involved in the degradation of the unassembled Na,K-ATPase alpha subunit and in its stabilization by beta subunit assembly[Ｊ].Mol Biol Cell,2000,11(5):1657-1672..
  10. 10. Pestov NB,Korneenko TV,Radkov R,et al.Identification of the β-subunit for nongastric H-K-ATPase in rat anterior prostate[Ｊ].Am J Physiol Cell Physiol,2004,286(6):C1229-C1237..
  11. 11. Rajasekaran SA,Gopal J,Willis D,et al.Na,K-ATPase beta1-subunit increases the translation efficiency of the alpha1-subunit in MSV-MDCK cells[Ｊ].Mol Biol Cell,2004,15(7):3224-3232.