• 四川大學(xué)華西醫(yī)院腎臟內(nèi)科，四川成都 610041;

目的：觀察青蒿琥酯對脂多糖誘導(dǎo)的大鼠腎小球系膜細(xì)胞(glomerular mesangial cells,GMC)凋亡的影響。方法：用不同濃度的青蒿琥酯刺激脂多糖誘導(dǎo)增殖的大鼠腎小球系膜細(xì)胞，HE染色觀察凋亡細(xì)胞形態(tài)，DAPI熒光染色觀察細(xì)胞核凋亡情況，用流式細(xì)胞儀檢測細(xì)胞凋亡率。結(jié)果：青蒿琥酯對系膜細(xì)胞凋亡有明顯誘導(dǎo)作用，呈一定劑量依賴性。結(jié)論：青蒿琥酯能夠劑量依賴性地誘導(dǎo)大鼠系膜細(xì)胞凋亡。

引用本文： 馬行一,楊珉,米緒華,舒貝,馬云霞,吳蔚樺,曾艷,胡耀. 青蒿琥酯對脂多糖誘導(dǎo)的大鼠腎小球系膜細(xì)胞凋亡的影響. 華西醫(yī)學(xué), 2008, 23(1): 92-93. doi: 復(fù)制

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  1. 1. Harrison DJ.Cell death in the diseased glomerulus[J].Histopathology，1988，12(6)：679-683..
  2. 2. Baker AJ,Mooney A,Hughe-s J,et al.Mesangial cell apoptosis:the major mechanism for resolution of glomerular hypercellularity inexperimental mesangial proliferative nephritis[J].J Clin Invest,1994,94(5):2015-2116..
  3. 3. 謝彤，吳平,唐德炎，等.環(huán)磷酰胺在體外對大鼠腎小球系膜細(xì)胞增殖及凋亡的影響[J].中國病理生理雜志,2003,19(3):391-393..
  4. 4. Amore A,Emancipator SN,Cirina P,et al.Nitric oxide mediates cyclosporine-induced apoptosis in cultured renal cell[J].Kidney Int,2000,57(4):1549-1559..
  5. 5. 孫田美，梅長林，葛守一，等.洛伐他汀對系膜細(xì)胞凋亡及Bcl-2和Bax基因表達(dá)的影響[J].中華腎臟病雜志，2003,19(1):38-42..
  6. 6. Chen HH,Zhou HJ,Fang X.Inhibition of human cancer cell line growth and human umbilical vein endothelial cell angiogenesis by artemisinin derivatives in vitro[J].Pharmacol Res,2003,48(3):231-236..
  7. 7. Posner GH,Northrop J,Paik IH,et al.New chemical and biological aspects of artemisinin－derived trioxanedimers[J].Bioorg Med Chem,2002,10(1)：227-232..
  8. 8. Efferth T,Olbrich A,Bauer R，et al.mRNA expression profiles for the response of human tumor cell lines to the antimalarial drugs artesunate,arteether,and artemether[J].Biochem Pharmacol,2002,64(4):617-623..
  9. 9. Singh NP,Lai H.Selective toxicity of dihydroartemisinin and holotransferrin toward human breast cancer cells[J].Life Sci,2001,70(1)：49-56..
  10. 10. 方步武,來麗娜,林雅軍,等.青蒿琥酯抗四氯化碳致大鼠肝纖維化的作用[J].中國藥理學(xué)通報，2005，21（6）：762-763.