• 1南華大學(xué)附二院眼科，湖南衡陽(yáng) 421000;2四川大學(xué)華西醫(yī)院眼科,眼科學(xué)研究室，四川成都 610041;3首都醫(yī)科大學(xué)同仁醫(yī)院眼科;

目的：本研究通過(guò)觀察經(jīng)地塞米松處理前后培養(yǎng)的人小梁細(xì)胞的變化,探討激素性青光眼房水排出阻力增加的機(jī)制。方法：將人類小梁細(xì)胞培養(yǎng)至接近體內(nèi)的高分化狀態(tài)，用地塞米松處理后,觀察細(xì)胞形態(tài)的變化，并檢測(cè)以下蛋白的分布和表達(dá)：(1)myocilin/TIGR蛋白；(2)纖連蛋白；(3)肌動(dòng)蛋白交聯(lián)網(wǎng)的形成；(4)血清淀粉樣物質(zhì)A蛋白。結(jié)果：人小梁細(xì)胞經(jīng)地塞米松處理后(1)胞體變大，排列不規(guī)則，邊界模糊，呈“融合”狀；(2)細(xì)胞內(nèi)外myocilin/TIGR蛋白表達(dá)均明顯增加，其胞外表達(dá)與纖連蛋白位置一致,提示相互作用；(3)纖連蛋白表達(dá)增加；(4)CLANs形成，細(xì)胞間連接增強(qiáng)；(5)血清淀粉樣物質(zhì)A蛋白表達(dá)增加。結(jié)論：人類小梁細(xì)胞經(jīng)地塞米松處理后形態(tài)發(fā)生變化，可能與應(yīng)力纖維變化及myocilin蛋白在細(xì)胞內(nèi)積聚有關(guān)。細(xì)胞間邊界不清，與細(xì)胞外基質(zhì)蛋白過(guò)度表達(dá)、沉積有關(guān)。小梁細(xì)胞外基質(zhì)沉積以及異常的細(xì)胞間連接等改變與房水排出阻力增加的病理過(guò)程有關(guān)。

引用本文： 譚娟,曹桂群,郭琳,余曼,崔敏,閆乃紅,王寧利,陳曉明,劉旭陽(yáng). 激素性青光眼房水排出阻力增加的機(jī)制探討. 華西醫(yī)學(xué), 2008, 23(1): 67-69. doi: 復(fù)制

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1.
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4. [14]Clark AF,Brotchie D,Read AT,Hellberg P,English-Wright S,Pang IH,Ethier CR,Grierson I.Dexamethasone alters F-actin architecture and promotes cross-linkedactin network formation in human trabecular meshwork tissue[J].Cell Motil Cytoskeleton,2005,60(2):83-95.
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6. [16]Clark AF,Lane D,Wilson K,et al.Inhibition of dexamethasone-induced cytoskeletal changes in cultured human trabecular meshwork cells by tetrahydrocortisol[J].Invest Ophthalmol Vis Sci,1996,37(5):805-813.
7. [17]Matsumoto Y,Johnson DH.Dexamethasone decreases phagocytosis by human trabecular meshwork cells in situ[J].Invest Ophthalmol Vis Sci,1997,38(9):1902-1907.
8. [18]El Kebie,Jozsef L,Khreiss T,et al.Aspirin-triggered lipoxins override the apoptosis-delaying action of serum amyloid A in human neutrophils:a novel mechanism for resolution of inflammation[J].J Immunol,2007,179(1):616-22.
9. Bill A.Mechanisms and routes of aqueous humor drainage.In:Albert DM,Jakobiec FA,eds.Principles and practice of ophthalmology:basic sciences[M].Philadelphia:Saunders,1994：206-226.
10. Fine BS,Yanoff M,Stone RA.A clinicopathologic study of four cases of primary open-angle glaucoma compared to normal eyes[J].Am J Ophthalmol,1981：91:88-105.
11. Rohen JW.Why is intraocular pressure elevated in chronic simple glaucoma[J]?Ophthalmology,1983,90:758-765.
12. Alvarado J,Murphy C,Polansky J,et al.Age-related changes in trabecular meshwork cellularity[J].Invest Ophthalmol Vis Sci,1981,21:714-727.
13. Polansky JR,Alvarado JA.Cellular mechanisms influencing the aqueous humor outflow pathway.In:Albert DM,Jakobiec FA,eds.Principles and practice of ophthalmology:basic sciences[M].Philadelphia:Saunders,1994：226-251.
14. Zhou Z，Vollrath D．A cellular assay distinguishes normal and mutant TIGR／myocilin protein[J].Hum Mol Genet，1999，8(12):2221-2228.
15. Rozsa FW，Shimlzu S，Lichter PR，Johnson AT.Othman MI.Scott K，et a1.GLCIA mutations point to regions of potential functional importance on the TIGR/MYOC protein[J].Mol Vis,1998,4:20.
16. Steely HT，Browder SL,Julian MB,et al.The effects of dexamethasone on fibronectin expression in cultured human trabecular meshwork cells[J].Invest Ophthalmol Vis Sci,1992,33:2242-2250.
17. Dickerson JE Jr,Steely HT Jr,English-Wright SL,et al.The effect of dexamethasone on integrin and laminin expression in cultured human trabecular meshwork cells[J].Exp Eye Res,1998,66:731-738.
18. Kaufman PL,Erickson KA.Cytochalasin B and D dose-outflow facility response relationships in the cynomolgus monkey[J].Invest Ophthalmol Vis Sci,1982,23:646-650.
19. Liu X,Cai S,Glasser A,Volberg T,Polansky JR,Fauss DJ,Geiger B,Kaufman PL.Effect of H-7 on cultured human trabecular meshwork cells[J].Molecular Vision,2001,7:145-153.
20. Clark AF,Wilson K,McCartney MD,Miggans ST,Kunkle M,Howe W.Glucocorticoid-induced formation of cross-linked actin networks in cultured human trabecular m.
  1. 1.
  2. 2. eshwork cells[J].Invest Ophthalmol Vis Sci,1994,35(1):281-94.
  3. 3. [13]Gotlieb AI.The endothelial cytoskeleton:organization in normal and regenerating endothelium[J].Toxicol Pathol,1990,18(4 Pt 1):603-17.
  4. 4. [14]Clark AF,Brotchie D,Read AT,Hellberg P,English-Wright S,Pang IH,Ethier CR,Grierson I.Dexamethasone alters F-actin architecture and promotes cross-linkedactin network formation in human trabecular meshwork tissue[J].Cell Motil Cytoskeleton,2005,60(2):83-95.
  5. 5. [15]Clark AF,Wilson K,McCartney MD,et al.Glucocorticoid-induced formation of cross-linked actin networks in cultured human trabecular meshwork cells[J].Invest Ophthalmol Vis Sci,1994,35:281-294.
  6. 6. [16]Clark AF,Lane D,Wilson K,et al.Inhibition of dexamethasone-induced cytoskeletal changes in cultured human trabecular meshwork cells by tetrahydrocortisol[J].Invest Ophthalmol Vis Sci,1996,37(5):805-813.
  7. 7. [17]Matsumoto Y,Johnson DH.Dexamethasone decreases phagocytosis by human trabecular meshwork cells in situ[J].Invest Ophthalmol Vis Sci,1997,38(9):1902-1907.
  8. 8. [18]El Kebie,Jozsef L,Khreiss T,et al.Aspirin-triggered lipoxins override the apoptosis-delaying action of serum amyloid A in human neutrophils:a novel mechanism for resolution of inflammation[J].J Immunol,2007,179(1):616-22.
  9. 9. Bill A.Mechanisms and routes of aqueous humor drainage.In:Albert DM,Jakobiec FA,eds.Principles and practice of ophthalmology:basic sciences[M].Philadelphia:Saunders,1994：206-226.
  10. 10. Fine BS,Yanoff M,Stone RA.A clinicopathologic study of four cases of primary open-angle glaucoma compared to normal eyes[J].Am J Ophthalmol,1981：91:88-105.
  11. 11. Rohen JW.Why is intraocular pressure elevated in chronic simple glaucoma[J]?Ophthalmology,1983,90:758-765.
  12. 12. Alvarado J,Murphy C,Polansky J,et al.Age-related changes in trabecular meshwork cellularity[J].Invest Ophthalmol Vis Sci,1981,21:714-727.
  13. 13. Polansky JR,Alvarado JA.Cellular mechanisms influencing the aqueous humor outflow pathway.In:Albert DM,Jakobiec FA,eds.Principles and practice of ophthalmology:basic sciences[M].Philadelphia:Saunders,1994：226-251.
  14. 14. Zhou Z，Vollrath D．A cellular assay distinguishes normal and mutant TIGR／myocilin protein[J].Hum Mol Genet，1999，8(12):2221-2228.
  15. 15. Rozsa FW，Shimlzu S，Lichter PR，Johnson AT.Othman MI.Scott K，et a1.GLCIA mutations point to regions of potential functional importance on the TIGR/MYOC protein[J].Mol Vis,1998,4:20.
  16. 16. Steely HT，Browder SL,Julian MB,et al.The effects of dexamethasone on fibronectin expression in cultured human trabecular meshwork cells[J].Invest Ophthalmol Vis Sci,1992,33:2242-2250.
  17. 17. Dickerson JE Jr,Steely HT Jr,English-Wright SL,et al.The effect of dexamethasone on integrin and laminin expression in cultured human trabecular meshwork cells[J].Exp Eye Res,1998,66:731-738.
  18. 18. Kaufman PL,Erickson KA.Cytochalasin B and D dose-outflow facility response relationships in the cynomolgus monkey[J].Invest Ophthalmol Vis Sci,1982,23:646-650.
  19. 19. Liu X,Cai S,Glasser A,Volberg T,Polansky JR,Fauss DJ,Geiger B,Kaufman PL.Effect of H-7 on cultured human trabecular meshwork cells[J].Molecular Vision,2001,7:145-153.
  20. 20. Clark AF,Wilson K,McCartney MD,Miggans ST,Kunkle M,Howe W.Glucocorticoid-induced formation of cross-linked actin networks in cultured human trabecular m.