• 重慶醫(yī)科大學(xué)（重慶，400016） 1附屬第一醫(yī)院骨科，2 附屬永川醫(yī)院;

目的 　觀察鹽酸氨基葡萄糖對兔關(guān)節(jié)腔積血造成的軟骨損傷的保護作用。 方法　成年新西蘭大白兔50只，隨機分為A、B、C、D、E共5組，每組10只。A、B、C、D組采用自體靜脈血4 mL右側(cè)關(guān)節(jié)腔內(nèi)注射建立關(guān)節(jié)腔積血模型。建模后，A、B、C組分別給予25、82.5、500 mg/kg的鹽酸氨基葡萄糖灌胃，1次/d；D組為陽性對照組，以6 mL/kg蒸餾水灌胃，1次/d；E組為陰性對照組，以6 mL/kg蒸餾水灌胃，1次/d。灌胃8周后取材。肉眼大體觀察，光鏡下進行Mankin評分及沙紅O染色觀察，評估軟骨損傷程度；采用免疫組織化學(xué)法測定Ⅱ型膠原蛋白（COL Ⅱ）表達情況。 結(jié)果 　軟骨大體觀察和沙紅O染色組織學(xué)觀察顯示，A、B、C組與D組、E組之間差異均有統(tǒng)計學(xué)意義（P＜0.05）。免疫組織化學(xué)提示，A、B、C組COL Ⅱ表達明顯高于D組（P＜0.05）。 結(jié)論 　鹽酸氨基葡萄糖對兔關(guān)節(jié)腔積血導(dǎo)致的軟骨損傷有一定的保護治療作用，在防治關(guān)節(jié)腔積血導(dǎo)致的軟骨損傷的發(fā)生發(fā)展方面，具有一定的作用。

引用本文： 張施洋,張銘華,蔣電明,趙增輝,李維朝,譙波. 鹽酸氨基葡萄糖在兔關(guān)節(jié)腔積血中對關(guān)節(jié)軟骨保護作用的實驗研究. 華西醫(yī)學(xué), 2012, 27(12): 1788-1791. doi: 復(fù)制

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1. 　Roosendaal G, Vianen ME, Marx JJ, et al. Blood-induced joint damage: a human in vitro study[J]. Arthritis Rheum, 1999, 42(5): 1025-1032.
2. 　Lohmander LS, injury RL. Surgery and osteoarthrosis: truth or Consequences[J]. Acta Orthop Scand, 1994, 65(2): 605-609.
3. 　Da CC, Dowless GV. Glucosamine sulfate for osteoarthritis[J]. Ann Pharmaeother, 1998, 32(5): 580-587.
4. 　Roosendaal G, Tekoppele JM, Vianen ME, et al. Blood-induced joint damage: a canine in vitro study[J]. Arthritis Rheum, 1999, 42(5): 1033-1039.
5. 　Lipowitz AJ, Wong PL, Stevens JB. Synovial membrane changes after experimental transection of the cranial cruciate ligament in dogs[J]. Am J Vet Res, 1985, 46(5): 1166-1170.
6. 　Mankin HJ, Dorfman H, Lippiello L, et al. Biochemical and metabolic abnormalities in articular cartilage from osteo-arthritic human hips. II. Correlation of morphology with biochemical and metabolic data[J]. J Bone Joint Surg Am, 1971, 53(3): 523-537.
7. 　Michel JJ, Goris R, Marieke E, et al. Immature articular cartilage is more susceptible to blood-induced damage than mature articular cartilage:An in vivo animal study[J]. Arthritis Rheum, 2010, 48(2): 396-403.
8. 　Valentino LA. Blood-induced joint disease: the pathophysiology of hemophilic arthropathy[J]. J Thromb Haemost, 2010, 8(9): 1895-1902.
9. 　張繼春, 陳金棟, 陳百成, 等. 兔關(guān)節(jié)內(nèi)積血對關(guān)節(jié)軟骨影響的實驗研究[J]. 中國康復(fù)醫(yī)學(xué)雜志, 2007, 22(1): 31-33.
10. 　Hakobyan N. Kazarian T’valentino LA.Synovitis in a murine model of human factor Ⅷdeficiency[J]. Haemophilia, 2005, 11(3): 227-232.
11. 　Tan AH, Mitra AK, Chang PC, et al. Assessment of blood-induced cartilage damage in rabbit knees using scanning electron microscopy[J]. J Orthop Surg (Hong Kong), 2004, 12(2): 199-204.
12. 　Hakobyan N, Kazarian T, Valentino LA. Synovitis in a murine model of human factor Ⅷ deficiency[J]. Haemophilia, 2005, 11(3): 227-232.
13. 　傅欣, 林霖, 魏學(xué)磊, 等. 氨基葡萄糖和硫酸軟骨素聯(lián)合用藥對兔骨關(guān)節(jié)炎治療作用實驗研究[J]. 中國運動醫(yī)學(xué)雜志, 2008, 22(5): 588-592.
14. 　Piperno M, Reboul P, Hellio Le Graverand MP, et al. Glucosamine sulfate modulates dysregulated activities of human osteoarthritic chondrocytes in vitro[J]. Osteoarthritis Cartilage, 2000, 8(3): 207-212.
15. 　Nakamura H, Shibakawa A, Tanaka M, et al. Effects of glucosamine hydrochloride on the production of prostaglandin E2, nitric oxide and metalloproteases by chondrocytes and synoviocytes in osteoarthritis[J]. Clin Exp Rheumatol, 2004, 22(3): 293-299.
16. 　楊南萍, 李江濤, 陳永濤, 等. 多中心隨機雙盲雙模擬陽性藥平行對照臨床研究:評價國產(chǎn)鹽酸氨基葡萄糖片在治療骨關(guān)節(jié)炎中的有效性和安全性[J]. 華西醫(yī)學(xué), 2005, 20(4): 54-56.
17. 　Bassleer C, Rovati L, Franchimont P. Stimulation of proteoglycan production by glucosamine sulfate in chondrocytes isolated from human osteoarthritic articular cartilage in vitro[J]. Osteoarthritis Cartilage, 1998, 6(6): 427-434.
18. 　Qiu GX, Gao SN, Giacovelli G, et al. Efficacy and safety of glucosamine sulfate versus ibuprofen in patients with knee osteoarthritis[J]. Arzneimittelforschung, 1998, 48(5): 469-474.
19. 　王曉濱, 黃公怡, 薛慶云. 硫酸氨基葡萄糖抑制白介素1β誘導(dǎo)的人骨關(guān)節(jié)炎軟骨細胞合成一氧化氮研究[J]. 中華老年醫(yī)學(xué)雜志, 2012, 31(2): 140-143.
20. 　Largo R, Alvarez-Soria MA, Díez-Ortego I, et al. Glucosamine inhibits IL-1beta-induced NFkappaB activation in human osteoarthritic chondrocytes[J]. Osteoarthritis Cartilage, 2003, 11(4): 290-298.
21. 　Huser CA, Davies ME. Effect of a glucosamine derivative on impact-induced chondrocyte apoptosis in vitro. A preliminary report[J]. Osteoarthritis Cartilage, 2008, 16(1): 125-128.
  1. 1. 　Roosendaal G, Vianen ME, Marx JJ, et al. Blood-induced joint damage: a human in vitro study[J]. Arthritis Rheum, 1999, 42(5): 1025-1032.
  2. 2. 　Lohmander LS, injury RL. Surgery and osteoarthrosis: truth or Consequences[J]. Acta Orthop Scand, 1994, 65(2): 605-609.
  3. 3. 　Da CC, Dowless GV. Glucosamine sulfate for osteoarthritis[J]. Ann Pharmaeother, 1998, 32(5): 580-587.
  4. 4. 　Roosendaal G, Tekoppele JM, Vianen ME, et al. Blood-induced joint damage: a canine in vitro study[J]. Arthritis Rheum, 1999, 42(5): 1033-1039.
  5. 5. 　Lipowitz AJ, Wong PL, Stevens JB. Synovial membrane changes after experimental transection of the cranial cruciate ligament in dogs[J]. Am J Vet Res, 1985, 46(5): 1166-1170.
  6. 6. 　Mankin HJ, Dorfman H, Lippiello L, et al. Biochemical and metabolic abnormalities in articular cartilage from osteo-arthritic human hips. II. Correlation of morphology with biochemical and metabolic data[J]. J Bone Joint Surg Am, 1971, 53(3): 523-537.
  7. 7. 　Michel JJ, Goris R, Marieke E, et al. Immature articular cartilage is more susceptible to blood-induced damage than mature articular cartilage:An in vivo animal study[J]. Arthritis Rheum, 2010, 48(2): 396-403.
  8. 8. 　Valentino LA. Blood-induced joint disease: the pathophysiology of hemophilic arthropathy[J]. J Thromb Haemost, 2010, 8(9): 1895-1902.
  9. 9. 　張繼春, 陳金棟, 陳百成, 等. 兔關(guān)節(jié)內(nèi)積血對關(guān)節(jié)軟骨影響的實驗研究[J]. 中國康復(fù)醫(yī)學(xué)雜志, 2007, 22(1): 31-33.
  10. 10. 　Hakobyan N. Kazarian T’valentino LA.Synovitis in a murine model of human factor Ⅷdeficiency[J]. Haemophilia, 2005, 11(3): 227-232.
  11. 11. 　Tan AH, Mitra AK, Chang PC, et al. Assessment of blood-induced cartilage damage in rabbit knees using scanning electron microscopy[J]. J Orthop Surg (Hong Kong), 2004, 12(2): 199-204.
  12. 12. 　Hakobyan N, Kazarian T, Valentino LA. Synovitis in a murine model of human factor Ⅷ deficiency[J]. Haemophilia, 2005, 11(3): 227-232.
  13. 13. 　傅欣, 林霖, 魏學(xué)磊, 等. 氨基葡萄糖和硫酸軟骨素聯(lián)合用藥對兔骨關(guān)節(jié)炎治療作用實驗研究[J]. 中國運動醫(yī)學(xué)雜志, 2008, 22(5): 588-592.
  14. 14. 　Piperno M, Reboul P, Hellio Le Graverand MP, et al. Glucosamine sulfate modulates dysregulated activities of human osteoarthritic chondrocytes in vitro[J]. Osteoarthritis Cartilage, 2000, 8(3): 207-212.
  15. 15. 　Nakamura H, Shibakawa A, Tanaka M, et al. Effects of glucosamine hydrochloride on the production of prostaglandin E2, nitric oxide and metalloproteases by chondrocytes and synoviocytes in osteoarthritis[J]. Clin Exp Rheumatol, 2004, 22(3): 293-299.
  16. 16. 　楊南萍, 李江濤, 陳永濤, 等. 多中心隨機雙盲雙模擬陽性藥平行對照臨床研究:評價國產(chǎn)鹽酸氨基葡萄糖片在治療骨關(guān)節(jié)炎中的有效性和安全性[J]. 華西醫(yī)學(xué), 2005, 20(4): 54-56.
  17. 17. 　Bassleer C, Rovati L, Franchimont P. Stimulation of proteoglycan production by glucosamine sulfate in chondrocytes isolated from human osteoarthritic articular cartilage in vitro[J]. Osteoarthritis Cartilage, 1998, 6(6): 427-434.
  18. 18. 　Qiu GX, Gao SN, Giacovelli G, et al. Efficacy and safety of glucosamine sulfate versus ibuprofen in patients with knee osteoarthritis[J]. Arzneimittelforschung, 1998, 48(5): 469-474.
  19. 19. 　王曉濱, 黃公怡, 薛慶云. 硫酸氨基葡萄糖抑制白介素1β誘導(dǎo)的人骨關(guān)節(jié)炎軟骨細胞合成一氧化氮研究[J]. 中華老年醫(yī)學(xué)雜志, 2012, 31(2): 140-143.
  20. 20. 　Largo R, Alvarez-Soria MA, Díez-Ortego I, et al. Glucosamine inhibits IL-1beta-induced NFkappaB activation in human osteoarthritic chondrocytes[J]. Osteoarthritis Cartilage, 2003, 11(4): 290-298.
  21. 21. 　Huser CA, Davies ME. Effect of a glucosamine derivative on impact-induced chondrocyte apoptosis in vitro. A preliminary report[J]. Osteoarthritis Cartilage, 2008, 16(1): 125-128.